PMID- 32315974
OWN - NLM
STAT- MEDLINE
DCOM- 20210205
LR  - 20210205
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 1145
DP  - 2020 May 15
TI  - Determination of adenosine phosphates in mouse myocardium tissue by HPLC with UV 
      detection and using porous graphite carbon column.
PG  - 122110
LID - S1570-0232(20)30258-0 [pii]
LID - 10.1016/j.jchromb.2020.122110 [doi]
AB  - A high-performance liquid chromatography (HPLC) method with UV detection was 
      established and validated for the simultaneous determination of adenosine 
      triphosphate (ATP), adenosine diphosphate (ADP) and adenosine monophosphate (AMP) 
      in mouse myocardial tissues. After protein precipitation and compound extraction 
      with pre-cooled perchloric acid and the supernatant was centrifuged with the pH 
      value adjusted to 6.5-7.5, the analytes were separated on a porous graphitic 
      carbon LC column (4.6 mm x 100 mm, 5 mum) using gradient elution with a mobile 
      phase of 10 mmol/L borax solution, pH 9.18(A) and acetonitrile-tetrahydrofuran 
      (1:1, v/v) (B). The LC flow rate was 0.8 mL/min; the UV detection wavelength was 
      254 nm and the column temperature was maintained at 35  degrees C. ATP, ADP, and AMP were 
      separated and the intra-day relative standard deviations (RSDs) of peak area 
      repeatability were 1.3-2.5% (n = 6). The correlation coefficients of the 
      linearity between UV responses and adenosine phosphate concentrations were larger 
      than 0.9998 in all cases, within concentration ranges of 0.71-91.6 mug/mL for ATP, 
      1.3-81.5 mug/mL for ADP and 1.69-108.1 mug/mL for AMP. The limits of detection were 
      within 0.17-0.21 mug/mL. The average standard substance spiked-in recoveries were 
      93.6-104.7% (n = 3). The established HPLC method was successfully applied to 
      quantitate ATP, ADP, and AMP in mouse myocardial tissues.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Feng, Jia-Hua
AU  - Feng JH
AD  - School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 
      201203, China.
FAU - Wei, Ke-Zhao
AU  - Wei KZ
AD  - School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 
      201203, China.
FAU - Gao, Jian-Ping
AU  - Gao JP
AD  - School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 
      201203, China. Electronic address: zydgjp@aliyun.com.
FAU - Xu, Xu
AU  - Xu X
AD  - School of Chemical and Environmental Engineering, Shanghai Institute of 
      Technology, Shanghai 201418, China. Electronic address: xuxu@sit.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20200411
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 415SHH325A (Adenosine Monophosphate)
RN  - 61D2G4IYVH (Adenosine Diphosphate)
RN  - 7782-42-5 (Graphite)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
SB  - IM
MH  - Adenosine Diphosphate/*analysis/metabolism
MH  - Adenosine Monophosphate/*analysis/metabolism
MH  - Adenosine Triphosphate/*analysis/metabolism
MH  - Animals
MH  - Chromatography, High Pressure Liquid/instrumentation/*methods
MH  - Graphite/chemistry
MH  - Male
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Myocardium/*chemistry/metabolism
MH  - Porosity
OTO - NOTNLM
OT  - Adenosine phosphates
OT  - Energy metabolism
OT  - HPLC
OT  - Porous graphite carbon column
COIS- Declaration of Competing Interest The authors declare no conflicts of interest.
EDAT- 2020/04/22 06:00
MHDA- 2021/02/07 06:00
CRDT- 2020/04/22 06:00
PHST- 2020/02/17 00:00 [received]
PHST- 2020/04/06 00:00 [revised]
PHST- 2020/04/09 00:00 [accepted]
PHST- 2020/04/22 06:00 [pubmed]
PHST- 2021/02/07 06:00 [medline]
PHST- 2020/04/22 06:00 [entrez]
AID - S1570-0232(20)30258-0 [pii]
AID - 10.1016/j.jchromb.2020.122110 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2020 May 15;1145:122110. doi: 
      10.1016/j.jchromb.2020.122110. Epub 2020 Apr 11.