PMID- 32316353
OWN - NLM
STAT- MEDLINE
DCOM- 20210122
LR  - 20211204
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 21
IP  - 8
DP  - 2020 Apr 16
TI  - Chondrogenic Potential of Pellet Culture Compared to High-Density Culture on a 
      Bacterial Cellulose Hydrogel.
LID - 10.3390/ijms21082785 [doi]
LID - 2785
AB  - Cell-based approaches of cartilage lesions use different culture systems to 
      obtain optimal cell quality. Pellet cultures with high cellular density (HD) are 
      the gold standard to keep chondrocytes in a differentiated stage. Bacterial 
      cellulose (BC) hydrogel is discussed to prevent cellular aging and 
      dedifferentiation. The hypothesis of this study was that HD culture on BC 
      hydrogel (HD hydrogel) might reach the chondrogenic potential of pellet culture 
      (pellet). Human articular osteoarthritic (OA) and non-osteoarthritic (non-OA) 
      chondrocytes were cultured for seven days within pellets and compared to HD 
      hydrogel and HD polystyrene. Gene expression analysis and histological assessment 
      were performed. We observed no significant change of COL2A1 expression by the 
      culture system (pellet, HD hydrogel and HD polystyrene) but a significant change 
      of COL2A1/COL1A1-ratio, with the highest ratio in pellets. Chondrocytes on HD 
      hydrogel showed an elevated expression of MMP13 and on polystyrene an increased 
      expression of COL1A1 and MMP13. The patterns of gene expression changes observed 
      in OA and non-OA chondrocytes in reaction to the different culture systems were 
      similar in those two cell groups. Pellet cultures moreover formed a 
      histomorphologically superior neocartilage. Concluding, human chondrocytes kept 
      the potential to express COL2A1 in all HD culture systems. However, pellets 
      excelled in a higher COL2A1/COL1A1-ratio, a higher extracellular matrix deposit 
      and in not developing degeneration and dedifferentiation markers. This underlines 
      the superiority of pellet culture in maintaining the chondrogenic potential of 
      human chondrocytes in vitro.
FAU - Grigull, Nele Pascale
AU  - Grigull NP
AD  - Department of Orthopaedics, Physical Medicine and Rehabilitation, University 
      Hospital, LMU Munich, Marchioninistr. 15, 81377 Munich, Germany.
FAU - Redeker, Julia Isabelle
AU  - Redeker JI
AD  - Department of Orthopaedics, Physical Medicine and Rehabilitation, University 
      Hospital, LMU Munich, Marchioninistr. 15, 81377 Munich, Germany.
FAU - Schmitt, Barbel
AU  - Schmitt B
AD  - Department of Orthopaedics, Physical Medicine and Rehabilitation, University 
      Hospital, LMU Munich, Marchioninistr. 15, 81377 Munich, Germany.
FAU - Saller, Maximilian Michael
AU  - Saller MM
AUID- ORCID: 0000-0001-8774-7892
AD  - Experimental Surgery and Regenerative Medicine (ExperiMed), Department of 
      General, Trauma and Reconstructive Surgery, Ludwig-Maximilians-University (LMU), 
      Fraunhoferstrasse 20, 82152 Martinsried, Germany.
FAU - Schonitzer, Veronika
AU  - Schonitzer V
AD  - Experimental Surgery and Regenerative Medicine (ExperiMed), Department of 
      General, Trauma and Reconstructive Surgery, Ludwig-Maximilians-University (LMU), 
      Fraunhoferstrasse 20, 82152 Martinsried, Germany.
FAU - Mayer-Wagner, Susanne
AU  - Mayer-Wagner S
AUID- ORCID: 0000-0001-6391-4086
AD  - Department of Orthopaedics, Physical Medicine and Rehabilitation, University 
      Hospital, LMU Munich, Marchioninistr. 15, 81377 Munich, Germany.
LA  - eng
GR  - 4663,/Bayerisches Staatsministerium fur Wirtschaft, Infrastruktur, Verkehr und 
      Technologie/
GR  - 24-15/Promotionsstudium Forderung fur Forschung und Lehre (FoFoLe) LMU/
PT  - Journal Article
DEP - 20200416
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (ACAN protein, human)
RN  - 0 (Aggrecans)
RN  - 0 (COL2A1 protein, human)
RN  - 0 (Collagen Type I)
RN  - 0 (Collagen Type I, alpha 1 Chain)
RN  - 0 (Collagen Type II)
RN  - 0 (Hydrogels)
RN  - 0 (Polystyrenes)
RN  - 0 (SOX9 Transcription Factor)
RN  - 0 (SOX9 protein, human)
RN  - 9004-34-6 (Cellulose)
RN  - EC 3.4.24.- (Matrix Metalloproteinase 13)
SB  - IM
MH  - Aggrecans/genetics/metabolism
MH  - Cell Culture Techniques/*methods
MH  - Cells, Cultured
MH  - Cellulose/chemistry
MH  - Chondrocytes/cytology/metabolism/pathology
MH  - Collagen Type I/genetics/metabolism
MH  - Collagen Type I, alpha 1 Chain
MH  - Collagen Type II/genetics/metabolism
MH  - Gene Expression
MH  - Gluconacetobacter/metabolism
MH  - Humans
MH  - Hydrogels/*chemistry
MH  - Matrix Metalloproteinase 13/genetics/metabolism
MH  - Polystyrenes/chemistry
MH  - SOX9 Transcription Factor/genetics/metabolism
PMC - PMC7215943
OTO - NOTNLM
OT  - bacterial cellulose hydrogel
OT  - cartilage repair
OT  - chondrogenic potential
OT  - human cartilage
COIS- The authors declare no conflict of interest.
EDAT- 2020/04/23 06:00
MHDA- 2021/01/23 06:00
PMCR- 2020/04/01
CRDT- 2020/04/23 06:00
PHST- 2020/04/02 00:00 [received]
PHST- 2020/04/15 00:00 [accepted]
PHST- 2020/04/23 06:00 [entrez]
PHST- 2020/04/23 06:00 [pubmed]
PHST- 2021/01/23 06:00 [medline]
PHST- 2020/04/01 00:00 [pmc-release]
AID - ijms21082785 [pii]
AID - ijms-21-02785 [pii]
AID - 10.3390/ijms21082785 [doi]
PST - epublish
SO  - Int J Mol Sci. 2020 Apr 16;21(8):2785. doi: 10.3390/ijms21082785.