PMID- 32317514
OWN - NLM
STAT- MEDLINE
DCOM- 20210127
LR  - 20220414
IS  - 0974-5130 (Electronic)
IS  - 0377-4929 (Linking)
VI  - 63
IP  - 2
DP  - 2020 Apr-Jun
TI  - Dual-color dual-hapten in situ hybridization (D-DISH) - Comparison with 
      fluorescence in situ hybridization (FISH) for HER2/neu testing in breast cancer.
PG  - 194-199
LID - 10.4103/IJPM.IJPM_861_19 [doi]
AB  - CONTEXT: HER2/neu testing in breast cancer is a mandate due to availability of 
      trastuzumab, a monoclonal antibody targeted against this biomarker. Dual-color 
      dual-hapten in situ hybridization (D-DISH) is a new test for assessment of 
      HER2/neu gene overexpression on light microscopy. AIMS: This was a validation 
      study for D-DISH in our laboratory and was conducted to study the concordance 
      between fluorescence in situ hybridization (FISH) and D-DISH for HER2/neu testing 
      in breast cancer. MATERIALS AND METHODS: In all, 150 cases of invasive breast 
      carcinoma requested for FISH analysis were selected. Immunohistochemistry by 
      Ventana PATHWAY anti-HER2/neu (4B5) antibody, FISH by ZytoLight SPEC ERBB2/CEN17 
      Dual Color Probe, and D-DISH using the Ventana INFORM HER2 Dual ISH DNA Probe 
      Cocktail Assay was carried out. STATISTICAL ANALYSIS: Cohen's kappa coefficient 
      was used to calculate concordance between FISH and D-DISH assays. The ratios and 
      average number of signals were compared with Lin's concordance correlation 
      coefficient. RESULTS: About 93.1% of the cases showed concordance between FISH 
      and D-DISH results. Cohen's kappa correlation coefficient was 0.836, indicating 
      almost perfect level of agreement. Lin's concordance correlation coefficient (rhoc) 
      showed moderate strength of agreement for HER2/chromosome 17 ratios between FISH 
      and D-DISH assays (rhoc 0.9452). As per the American Society of Clinical 
      Oncology/College of American Pathologists (ASCO/CAP) 2018 updated guidelines, 
      four of the cases that were nonamplified on FISH showed low-level amplification 
      on D-DISH due to counting errors caused by faint signals or background dust. 
      Genomic heterogeneity and larger red chromosome 17 signals on D-DISH led to 
      discordance of the six cases amplified by FISH. D-DISH failure rate was 3.33%. 
      CONCLUSION: Overall, D-DISH showed good concordance with FISH but needs expertise 
      for reporting.
FAU - Gajaria, Pooja K
AU  - Gajaria PK
AD  - Department of Pathology, Tata Memorial Hospital, Mumbai, Maharashtra, India.
FAU - Tambe, Sonali
AU  - Tambe S
AD  - Department of Pathology, Tata Memorial Hospital, Mumbai, Maharashtra, India.
FAU - Pai, Trupti
AU  - Pai T
AD  - Department of Pathology, Tata Memorial Hospital, Mumbai, Maharashtra, India.
FAU - Patil, Asawari
AU  - Patil A
AD  - Department of Pathology, Tata Memorial Hospital, Mumbai, Maharashtra, India.
FAU - Desai, Sangeeta B
AU  - Desai SB
AD  - Department of Pathology, Tata Memorial Hospital, Mumbai, Maharashtra, India.
FAU - Shet, Tanuja M
AU  - Shet TM
AD  - Department of Pathology, Tata Memorial Hospital, Mumbai, Maharashtra, India.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Validation Study
PL  - India
TA  - Indian J Pathol Microbiol
JT  - Indian journal of pathology & microbiology
JID - 7605904
RN  - 0 (Haptens)
RN  - EC 2.7.10.1 (ERBB2 protein, human)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Breast Neoplasms/*diagnosis/pathology
MH  - *Color
MH  - Female
MH  - Haptens
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization/*methods
MH  - *In Situ Hybridization, Fluorescence
MH  - Paraffin Embedding
MH  - Receptor, ErbB-2/*genetics
OTO - NOTNLM
OT  - Concordance
OT  - HER2
OT  - dual-color dual-hapten in situ hybridization
OT  - fluorescence in situ hybridization
OT  - invasive breast carcinoma
OT  - validation
EDAT- 2020/04/23 06:00
MHDA- 2021/01/28 06:00
CRDT- 2020/04/23 06:00
PHST- 2020/04/23 06:00 [entrez]
PHST- 2020/04/23 06:00 [pubmed]
PHST- 2021/01/28 06:00 [medline]
AID - IndianJPatholMicrobiol_2020_63_2_194_282719 [pii]
AID - 10.4103/IJPM.IJPM_861_19 [doi]
PST - ppublish
SO  - Indian J Pathol Microbiol. 2020 Apr-Jun;63(2):194-199. doi: 
      10.4103/IJPM.IJPM_861_19.