PMID- 32317760
OWN - NLM
STAT- MEDLINE
DCOM- 20201207
LR  - 20210421
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 10
IP  - 1
DP  - 2020 Apr 21
TI  - Identification and molecular characterization of the first complete genome 
      sequence of Human Parechovirus type 15.
PG  - 6759
LID - 10.1038/s41598-020-63467-w [doi]
LID - 6759
AB  - Using a metagenomics approach, we have determined the first full-length genome 
      sequence of a human parechovirus type 15 (HPeV15) strain, isolated from a child 
      with acute flaccid paralysis and co-infected with EV-A71. HPeV15 is a rarely 
      reported type. To date, no full-length genome sequence of HPeV15 is available in 
      the GenBank database, where only limited VP1 sequences of this virus are 
      available. Pairwise comparisons of the complete VP1 nucleotide and deduced amino 
      acid sequences revealed that the study strain belongs to type 15 as it displayed 
      79.6% nucleotide and 93.4% amino acid identity with the HPeV15 prototype strain. 
      Comparative analysis of available genomic regions and phylogenetic analysis using 
      the P2 and P3 coding regions revealed low nucleotide identity to HPeV reference 
      genomes. Phylogenetic and similarity plot analyses showed that genomic 
      recombination events might have occurred in the UTRs and nonstructural region 
      during HPeV15 evolution. The study strain has high similarity features with 
      different variants of HPeV3 suggesting intertypic recombination. Our data 
      contributes to the scarce data available on HPeVs in Africa and provides valuable 
      information for future studies that aim to understand the evolutionary history, 
      molecular epidemiology or biological and pathogenic properties of HPeV15.
FAU - Fernandez-Garcia, Maria Dolores
AU  - Fernandez-Garcia MD
AUID- ORCID: 0000-0003-0336-6596
AD  - Institut Pasteur, Dakar, Senegal. dolores.fernandez@yahoo.es.
FAU - Simon-Loriere, Etienne
AU  - Simon-Loriere E
AUID- ORCID: 0000-0001-8420-7743
AD  - Institut Pasteur Paris, Paris, France.
FAU - Kebe, Ousmane
AU  - Kebe O
AD  - Institut Pasteur, Dakar, Senegal.
FAU - Sakuntabhai, A
AU  - Sakuntabhai A
AUID- ORCID: 0000-0001-9099-6937
AD  - Institut Pasteur Paris, Paris, France.
FAU - Ndiaye, Kader
AU  - Ndiaye K
AD  - Institut Pasteur, Dakar, Senegal.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200421
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
SB  - IM
MH  - Genome, Viral/*genetics
MH  - Genomics
MH  - Humans
MH  - Metagenomics/methods
MH  - Molecular Sequence Annotation
MH  - Open Reading Frames/genetics
MH  - Paralysis/*genetics/virology
MH  - Parechovirus/*genetics/isolation & purification/pathogenicity
MH  - Sequence Analysis, DNA
MH  - *Whole Genome Sequencing
PMC - PMC7174385
COIS- The authors declare no competing interests.
EDAT- 2020/04/23 06:00
MHDA- 2020/12/15 06:00
PMCR- 2020/04/21
CRDT- 2020/04/23 06:00
PHST- 2019/10/01 00:00 [received]
PHST- 2020/03/26 00:00 [accepted]
PHST- 2020/04/23 06:00 [entrez]
PHST- 2020/04/23 06:00 [pubmed]
PHST- 2020/12/15 06:00 [medline]
PHST- 2020/04/21 00:00 [pmc-release]
AID - 10.1038/s41598-020-63467-w [pii]
AID - 63467 [pii]
AID - 10.1038/s41598-020-63467-w [doi]
PST - epublish
SO  - Sci Rep. 2020 Apr 21;10(1):6759. doi: 10.1038/s41598-020-63467-w.