PMID- 32318029
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 11
DP  - 2020
TI  - A Novel wx2 Gene of Toxoplasma gondii Inhibits the Parasitic Invasion and 
      Proliferation in vitro and Attenuates Virulence in vivo via Immune Response 
      Modulation.
PG  - 399
LID - 10.3389/fmicb.2020.00399 [doi]
LID - 399
AB  - Toxoplasma gondii (T. gondii) is an obligate intracellular apicomplexan protozoan 
      that can parasitize most warm-blooded animals and cause severe diseases in 
      immunocompromised individuals or fetal abnormalities in pregnant woman. The 
      treatment of toxoplamosis has been limited by effective drugs. Our previous work 
      indicated that the novel gene wx2 of T. gondii may serve as a vaccine antigen 
      candidate. To further investigate the molecular functions of wx2 in highly 
      virulent T. gondii (RH strain), a wx2 gene deletion mutant RH strain (KO-wx2) was 
      established using CRISPR-Cas9. The phynotype of KO-wx2 was analyzed by plaque, 
      invasion, and replication assays in vitro as well as in vivo virulence assays. 
      The results indicated that the targeted deletion of the wx2 gene significantly 
      inhibited in vitro parasite growth and replication in the host cells as well as 
      attenuated parasite virulence in the mouse model. Notably, the percentage of 
      pro-inflammatory factors of interferon gamma (IFN-gamma) and interlukin-17A (IL-17A) 
      and anti-inflammatory factor of interlukin-10 (IL-10) in the lymph nodes were 
      upregulated in mice infected with the KO-wx2 strain. Our data suggested that the 
      wx2 gene plays an important role in the process of the parasite's life cycle and 
      virulence in mice. In addition, it also plays an important role in the host's 
      immunity reaction, mainly via Th1 and Th17 cellular immunity, not Th2.
CI  - Copyright (c) 2020 Ma, Yan, Jiang, Guan, Yang, Huang, Lu, Li, Zhang, Chang and Wu.
FAU - Ma, Zhenrong
AU  - Ma Z
AD  - Department of Parasitology, Xiangya School of Basic Medicine, Central South 
      University, Changsha, China.
FAU - Yan, Kang
AU  - Yan K
AD  - Department of Parasitology, Xiangya School of Basic Medicine, Central South 
      University, Changsha, China.
FAU - Jiang, Ruolan
AU  - Jiang R
AD  - Department of Parasitology, Xiangya School of Basic Medicine, Central South 
      University, Changsha, China.
FAU - Guan, Jie
AU  - Guan J
AD  - Department of Parasitology, Xiangya School of Basic Medicine, Central South 
      University, Changsha, China.
FAU - Yang, Linfei
AU  - Yang L
AD  - Department of Parasitology, Xiangya School of Basic Medicine, Central South 
      University, Changsha, China.
FAU - Huang, Yehong
AU  - Huang Y
AD  - Department of Parasitology, Xiangya School of Basic Medicine, Central South 
      University, Changsha, China.
FAU - Lu, Bin
AU  - Lu B
AD  - Department of Parasitology, Xiangya School of Basic Medicine, Central South 
      University, Changsha, China.
FAU - Li, Xuanwu
AU  - Li X
AD  - Department of Parasitology, Xiangya School of Basic Medicine, Central South 
      University, Changsha, China.
FAU - Zhang, Jie
AU  - Zhang J
AD  - Department of Parasitology, Xiangya School of Basic Medicine, Central South 
      University, Changsha, China.
FAU - Chang, Yunfeng
AU  - Chang Y
AD  - Department of Forensic Medicine Science, Central South University, Changsha, 
      China.
FAU - Wu, Xiang
AU  - Wu X
AD  - Department of Parasitology, Xiangya School of Basic Medicine, Central South 
      University, Changsha, China.
LA  - eng
PT  - Journal Article
DEP - 20200407
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
EIN - Front Microbiol. 2020 Aug 20;11:1882. PMID: 32973698
PMC - PMC7154108
OTO - NOTNLM
OT  - CRISPR-Cas9 system
OT  - Toxoplasma gondii
OT  - gene functions
OT  - gene knockout
OT  - immune response
OT  - virulence factors
OT  - wx2 gene
EDAT- 2020/04/23 06:00
MHDA- 2020/04/23 06:01
PMCR- 2020/04/07
CRDT- 2020/04/23 06:00
PHST- 2019/11/26 00:00 [received]
PHST- 2020/02/26 00:00 [accepted]
PHST- 2020/04/23 06:00 [entrez]
PHST- 2020/04/23 06:00 [pubmed]
PHST- 2020/04/23 06:01 [medline]
PHST- 2020/04/07 00:00 [pmc-release]
AID - 10.3389/fmicb.2020.00399 [doi]
PST - epublish
SO  - Front Microbiol. 2020 Apr 7;11:399. doi: 10.3389/fmicb.2020.00399. eCollection 
      2020.