PMID- 32319197 OWN - NLM STAT- MEDLINE DCOM- 20210512 LR - 20210512 IS - 1756-185X (Electronic) IS - 1756-1841 (Linking) VI - 23 IP - 6 DP - 2020 Jun TI - Intra-articular delivery of umbilical cord-derived mesenchymal stem cells temporarily retard the progression of osteoarthritis in a rat model. PG - 778-787 LID - 10.1111/1756-185X.13834 [doi] AB - AIM: Mesenchymal stem cell (MSC)-based therapy is being explored in treating osteoarthritis (OA). Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) are least reported. In this study, we investigated the effects of single intra-articular injections of hUC-MSCs on a rat OA model. METHOD: hUC-MSCs were isolated from the Wharton's jelly of the human umbilical cord and identified. Eighteen Sprague-Dawley rats were used for the OA model. All rats were divided into 3 groups: hyaluronic acid (HA)+MSCs (n = 6), HA (n = 6), and control group (n = 6). One by 10(6) hUC-MSCs in 100 muL HA, 100 muL HA or 100 muL saline were injected into the knee joint 4 weeks post-surgery as a single dose. Cartilage degeneration was evaluated at 6 and 12 weeks after treatment with macroscopic examination, micro-computed tomography analysis, behavioral analysis, and histology. RESULTS: At 6 weeks, the HA + MSCs group had a significantly better International Cartilage Repair Society score in the femoral condyle compared to the HA and control groups. Histological analysis also showed more proteoglycan and less cartilage loss, with lower modified Mankin score in the HA + MSCs group. However, at 12 weeks there were no significant differences between groups from macroscopic examination and histological analysis. Subchondral bone sclerosis of the medial femoral condyle and behavioral tests showed no significant differences between groups at 6 and 12 weeks. CONCLUSION: These findings indicate that single injection of hUC-MSCs can have temporary effects on decelerating the progression of cartilage degeneration in OA rats, but may not inhibit OA progression in the long-term. CI - (c) 2020 Asia Pacific League of Associations for Rheumatology and John Wiley & Sons Australia, Ltd. FAU - Xing, Dan AU - Xing D AUID- ORCID: 0000-0001-6966-3134 AD - Arthritis Clinic & Research Center, Peking University People's Hospital, Peking University, Beijing, China. AD - Arthritis Institute, Peking University, Beijing, China. FAU - Wu, Jun AU - Wu J AD - State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China. AD - Beijing Stem Cell Bank, Chinese Academy of Sciences, Beijing, China. AD - University of Chinese Academy of Sciences, Beijing, China. FAU - Wang, Bin AU - Wang B AUID- ORCID: 0000-0002-5474-1002 AD - Department of Sports Medicine and Adult Reconstruction Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, China. FAU - Liu, Wei AU - Liu W AD - Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, China. AD - Beijing CytoNiche Biotechnology Co. Ltd., Beijing, China. FAU - Liu, Wenjing AU - Liu W AD - State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China. AD - Beijing Stem Cell Bank, Chinese Academy of Sciences, Beijing, China. FAU - Zhao, Yu AU - Zhao Y AD - Arthritis Clinic & Research Center, Peking University People's Hospital, Peking University, Beijing, China. AD - Arthritis Institute, Peking University, Beijing, China. FAU - Wang, Liu AU - Wang L AD - State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China. AD - Beijing Stem Cell Bank, Chinese Academy of Sciences, Beijing, China. AD - University of Chinese Academy of Sciences, Beijing, China. AD - Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China. FAU - Li, Jiao Jiao AU - Li JJ AD - Kolling Institute, University of Sydney, Sydney, NSW, Australia. FAU - Liu, Aifeng AU - Liu A AD - Department of Orthopedics, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, China. FAU - Zhou, Qi AU - Zhou Q AD - State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China. AD - Beijing Stem Cell Bank, Chinese Academy of Sciences, Beijing, China. AD - University of Chinese Academy of Sciences, Beijing, China. AD - Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China. FAU - Hao, Jie AU - Hao J AD - State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China. AD - Beijing Stem Cell Bank, Chinese Academy of Sciences, Beijing, China. AD - University of Chinese Academy of Sciences, Beijing, China. FAU - Lin, Jianhao AU - Lin J AUID- ORCID: 0000-0003-1830-9244 AD - Arthritis Clinic & Research Center, Peking University People's Hospital, Peking University, Beijing, China. AD - Arthritis Institute, Peking University, Beijing, China. LA - eng GR - Z181100003818005/Beijing Municipal Science &Technology Commission Huairou Science City Project/ GR - Z181100001818008/Beijing Science and Technology Major Projects/ GR - RDY2019-11/Peking University People's Hospital Scientific Research Development Funds/ GR - 81973606/National Natural Science Foundation of China/ GR - 81873316/National Natural Science Foundation of China/ GR - 81673994/National Natural Science Foundation of China/ GR - 2017M620803/the China Postdoctoral Science Foundation/ GR - XDA16030601/Strategic Priority Research Program of the Chinese Academy of Sciences/ PT - Journal Article DEP - 20200421 PL - England TA - Int J Rheum Dis JT - International journal of rheumatic diseases JID - 101474930 SB - IM MH - Animals MH - Disease Models, Animal MH - Disease Progression MH - Injections, Intra-Articular MH - Knee Joint MH - Mesenchymal Stem Cell Transplantation/*methods MH - Osteoarthritis, Knee/diagnosis/*therapy MH - Rats MH - Rats, Sprague-Dawley MH - Treatment Outcome MH - Umbilical Cord/*cytology MH - X-Ray Microtomography OTO - NOTNLM OT - animal study OT - cartilage regeneration OT - intra-articular injection OT - mesenchymal stem cells OT - osteoarthritis EDAT- 2020/04/23 06:00 MHDA- 2021/05/13 06:00 CRDT- 2020/04/23 06:00 PHST- 2019/08/21 00:00 [received] PHST- 2020/02/27 00:00 [revised] PHST- 2020/03/05 00:00 [accepted] PHST- 2020/04/23 06:00 [pubmed] PHST- 2021/05/13 06:00 [medline] PHST- 2020/04/23 06:00 [entrez] AID - 10.1111/1756-185X.13834 [doi] PST - ppublish SO - Int J Rheum Dis. 2020 Jun;23(6):778-787. doi: 10.1111/1756-185X.13834. Epub 2020 Apr 21.