PMID- 32319429
OWN - NLM
STAT- MEDLINE
DCOM- 20210205
LR  - 20210205
IS  - 1643-3750 (Electronic)
IS  - 1234-1010 (Print)
IS  - 1234-1010 (Linking)
VI  - 26
DP  - 2020 Apr 22
TI  - Progress in Research on Sperm DNA Fragmentation.
PG  - e918746
LID - 10.12659/MSM.918746 [doi]
AB  - With the increasing incidence of male infertility, routine detection of semen is 
      insufficient to accurately assess male fertility. Infertile men, who have lower 
      odds of conceiving naturally, exhibit high levels of sperm DNA fragmentation 
      (SDF). The mechanisms driving SDF include abnormal spermatogenesis, oxidative 
      stress damage, and abnormal sperm apoptosis. As these factors can induce SDF and 
      subsequent radical changes leading to male infertility, detection of the extent 
      of SDF has become an efficient routine method for semen analysis. Although it is 
      still debated, SDF detection has become a research hotspot in the field of 
      reproductive medicine as a more accurate indicator for assessing sperm quality 
      and male fertility. SDF may be involved in male infertility, reproductive 
      assisted outcomes, and growth and development of offspring. The effective 
      detection methods of SDF are sperm chromatin structure analysis (SCSA), terminal 
      transferase-mediated dUTP end labeling (TUNEL) assay, single-cell gel 
      electrophoresis (SCGE) assay, and sperm chromatin dispersion (SCD) test, and all 
      of these methods are valuable for assisted reproductive techniques. Currently, 
      the preferred method for detecting sperm DNA integrity is SCSA. However, the 
      regulation network of SDF is very complex because the sperm DNA differs from the 
      somatic cell DNA with its unique structure. A multitude of molecular factors, 
      including coding genes, non-coding genes, or methylated DNA, participate in the 
      complex physiological regulation activities associated with SDF. Studying SDF 
      occurrence and the underlying mechanisms may effectively improve its clinical 
      treatments. This review aimed to outline the research status of SDF mechanism and 
      detection technology-related issues, as well as the effect of increased SDF rate, 
      aiming to provide a basis for clinical male infertility diagnosis and treatment.
FAU - Qiu, Ying
AU  - Qiu Y
AD  - The Reproductive Medical Center, Nanning Second People's Hospital, Nanning, 
      Guangxi, China (mainland).
FAU - Yang, Hua
AU  - Yang H
AD  - The Reproductive Medical Center, Nanning Second People's Hospital, Nanning, 
      Guangxi, China (mainland).
FAU - Li, Chunyuan
AU  - Li C
AD  - The Reproductive Medical Center, Nanning Second People's Hospital, Nanning, 
      Guangxi, China (mainland).
FAU - Xu, Changlong
AU  - Xu C
AD  - The Reproductive Medical Center, Nanning Second People's Hospital, Nanning, 
      Guangxi, China (mainland).
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20200422
PL  - United States
TA  - Med Sci Monit
JT  - Medical science monitor : international medical journal of experimental and 
      clinical research
JID - 9609063
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - DNA/genetics
MH  - DNA Fragmentation
MH  - Humans
MH  - Infertility, Male/*genetics/metabolism
MH  - Male
MH  - Reproductive Techniques, Assisted
MH  - Semen/cytology/metabolism
MH  - Semen Analysis/*methods
MH  - Spermatozoa/*cytology
PMC - PMC7191954
EDAT- 2020/04/23 06:00
MHDA- 2021/02/07 06:00
PMCR- 2020/04/22
CRDT- 2020/04/23 06:00
PHST- 2020/04/23 06:00 [entrez]
PHST- 2020/04/23 06:00 [pubmed]
PHST- 2021/02/07 06:00 [medline]
PHST- 2020/04/22 00:00 [pmc-release]
AID - 918746 [pii]
AID - 10.12659/MSM.918746 [doi]
PST - epublish
SO  - Med Sci Monit. 2020 Apr 22;26:e918746. doi: 10.12659/MSM.918746.