PMID- 32321154
OWN - NLM
STAT- MEDLINE
DCOM- 20200506
LR  - 20200506
IS  - 1678-8060 (Electronic)
IS  - 0074-0276 (Print)
IS  - 0074-0276 (Linking)
VI  - 115
DP  - 2020
TI  - DNA nanovaccines prepared using LemA antigen protect Golden Syrian hamsters 
      against Leptospira lethal infection.
PG  - e190396
LID - S0074-02762020000100311 [pii]
LID - 10.1590/0074-02760190396 [doi]
LID - e190396
AB  - BACKGROUND: Nanoparticles (NPs) are viable candidates as carriers of exogenous 
      materials into cells via transfection and can be used in the DNA vaccination 
      strategy against leptospirosis. OBJECTIVES: We evaluated the efficiency of 
      halloysite clay nanotubes (HNTs) and amine-functionalised multi-walled carbon 
      nanotubes (NH2-MWCNTs) in facilitating recombinant LemA antigen (rLemA) 
      expression and protecting Golden Syrian hamsters (Mesocricetus auratus) against 
      Leptospira interrogans lethal infection. METHODS: An indirect immunofluorescent 
      technique was used to investigate the potency of HNTs and NH2-MWCNTs in enhancing 
      the transfection and expression efficiency of the DNA vaccine in Chinese hamster 
      ovary (CHO) cells. Hamsters were immunised with two doses of vaccines 
      HNT-pTARGET/lemA, NH2-MWCNTs-pTARGET/lemA, pTARGET/lemA, and empty pTARGET 
      (control), and the efficacy was determined in terms of humoral immune response 
      and protection against a lethal challenge. FINDINGS: rLemA DNA vaccines carried 
      by NPs were able to transfect CHO cells effectively, inducing IgG immune response 
      in hamsters (p < 0.05), and did not exhibit cytotoxic effects. Furthermore, 83.3% 
      of the hamsters immunised with NH2-MWCNTs-pTARGET/lemA were protected against the 
      lethal challenge (p < 0.01), and 66.7% of hamsters immunised with 
      HNT-pTARGET/lemA survived (p < 0.05). MAIN CONCLUSIONS: NH2-MWCNTs and HNTs can 
      act as antigen carriers for mammalian cells and are suitable for DNA nanovaccine 
      delivery.
FAU - Oliveira, Thais Larre
AU  - Oliveira TL
AD  - Universidade Federal de Pelotas, Centro de Desenvolvimento Tecnologico, Programa 
      de Pos-Graduacao em Biotecnologia, Nucleo de Biotecnologia, Pelotas, RS, Brasil.
FAU - Bacelo, Katia Leston
AU  - Bacelo KL
AD  - Universidade Federal de Pelotas, Centro de Desenvolvimento Tecnologico, Programa 
      de Pos-Graduacao em Biotecnologia, Nucleo de Biotecnologia, Pelotas, RS, Brasil.
FAU - Forster, Karine Maciel
AU  - Forster KM
AD  - Universidade Federal de Pelotas, Centro de Desenvolvimento Tecnologico, Programa 
      de Pos-Graduacao em Biotecnologia, Nucleo de Biotecnologia, Pelotas, RS, Brasil.
FAU - Ilha, Vinicius
AU  - Ilha V
AD  - Universidade Federal de Santa Maria, Departamento de Quimica, Santa Maria, RS, 
      Brasil.
FAU - Rodrigues, Oscar Endrigo
AU  - Rodrigues OE
AD  - Universidade Federal de Santa Maria, Departamento de Quimica, Santa Maria, RS, 
      Brasil.
FAU - Hartwig, Daiane D
AU  - Hartwig DD
AUID- ORCID: 0000-0003-3604-0832
AD  - Universidade Federal de Pelotas, Centro de Desenvolvimento Tecnologico, Programa 
      de Pos-Graduacao em Biotecnologia, Nucleo de Biotecnologia, Pelotas, RS, Brasil.
AD  - Universidade Federal de Pelotas, Instituto de Biologia, Departamento de 
      Microbiologia e Parasitologia, Pelotas, RS, Brasil.
LA  - eng
PT  - Journal Article
DEP - 20200417
PL  - Brazil
TA  - Mem Inst Oswaldo Cruz
JT  - Memorias do Instituto Oswaldo Cruz
JID - 7502619
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Bacterial Vaccines)
RN  - 0 (Transcription Factors)
RN  - 0 (Vaccines, DNA)
RN  - EC 2.7.3.- (lemA protein, bacterial)
SB  - IM
MH  - Animals
MH  - Antibodies, Bacterial/immunology
MH  - Antigens, Bacterial/*administration & dosage/immunology
MH  - Bacterial Proteins/*administration & dosage/immunology
MH  - Bacterial Vaccines/*administration & dosage/immunology
MH  - Cricetinae
MH  - Disease Models, Animal
MH  - Female
MH  - Fluorescent Antibody Technique, Indirect
MH  - Leptospira interrogans/immunology
MH  - Leptospirosis/immunology/*prevention & control
MH  - Nanoparticles
MH  - Transcription Factors/*administration & dosage/immunology
MH  - Vaccines, DNA/*administration & dosage/immunology
PMC - PMC7164400
EDAT- 2020/04/23 06:00
MHDA- 2020/05/07 06:00
PMCR- 2020/04/17
CRDT- 2020/04/23 06:00
PHST- 2019/10/28 00:00 [received]
PHST- 2020/03/03 00:00 [accepted]
PHST- 2020/04/23 06:00 [entrez]
PHST- 2020/04/23 06:00 [pubmed]
PHST- 2020/05/07 06:00 [medline]
PHST- 2020/04/17 00:00 [pmc-release]
AID - S0074-02762020000100311 [pii]
AID - 10.1590/0074-02760190396 [doi]
PST - epublish
SO  - Mem Inst Oswaldo Cruz. 2020 Apr 17;115:e190396. doi: 10.1590/0074-02760190396. 
      eCollection 2020.