PMID- 32321168
OWN - NLM
STAT- MEDLINE
DCOM- 20201113
LR  - 20230216
IS  - 1541-6100 (Electronic)
IS  - 0022-3166 (Linking)
VI  - 150
IP  - 7
DP  - 2020 Jul 1
TI  - Consumption of the Artificial Sweetener Acesulfame Potassium throughout Pregnancy 
      Induces Glucose Intolerance and Adipose Tissue Dysfunction in Mice.
PG  - 1773-1781
LID - 10.1093/jn/nxaa106 [doi]
AB  - BACKGROUND: Sugar-sweetened beverage consumption is associated with metabolic 
      dysfunction. Artificially sweetened beverages (ASBs) are often promoted as an 
      alternative. However, evidence for the safety of ASB consumption during pregnancy 
      is lacking. OBJECTIVES: The effects of sugar-sweetened beverage and ASB 
      consumption during pregnancy in mice were examined, and we hypothesized that both 
      sugar-sweetened beverages and ASBs would impair maternal metabolic function. 
      METHODS: Pregnant female C57BL/6J mice received control drinking water (CD), 
      high-fructose corn syrup (Fr; 20% kcal intake; 335 mM), or the artificial 
      sweetener acesulfame potassium (AS; 12.5 mM) in their drinking water, from 
      gestational day (GD) 0.5 (n = 8/group). Body weights and food and water intakes 
      were assessed every second day, an oral-glucose-tolerance test (OGTT) was 
      performed at GD 16.5, and mice were culled at GD 18.5. RT-PCR was carried out on 
      adipose tissue, liver, and gut. Adipose tissue morphology was assessed using 
      histological methods. In a separate cohort of animals, pregnancy length was 
      assessed. Repeated-measures ANOVA was performed for the OGTT and weight gain 
      data. All other data were analyzed by 1-way ANOVA. RESULTS: Fr and AS 
      significantly impaired glucose tolerance, as demonstrated by OGTT (21% and 24% 
      increase in AUC, respectively; P = 0.0006). Fr and AS reduced expression of 
      insulin receptor (39.5% and 33% reduction, respectively; P = 0.02) and peroxisome 
      proliferator-activated receptor gamma (45.2% and 47%, respectively; P = 0.039), 
      whereas Fr alone reduced expression of protein kinase B (36.9% reduction; 
      P = 0.048) and resulted in an increase in adipocyte size and leptin 
      concentrations (40% increase; P = 0.03). AS, but not Fr, reduced male fetal 
      weight (16.5% reduction; P = 0.04) and female fetal fasting blood glucose 
      concentration at cull (20% reduction; P = 0.02) compared with CD. AS 
      significantly reduced the length of pregnancy compared with the CD and Fr groups 
      (1.25 d shorter; P = 0.02). CONCLUSIONS: Fr and AS consumption were associated 
      with maternal metabolic dysfunction in mice. AS was also associated with reduced 
      fetal growth and fetal hypoglycemia. Therefore, ASBs may not be a beneficial 
      alternative to sugar-sweetened beverages during pregnancy.
CI  - Copyright (c) The Author(s) on behalf of the American Society for Nutrition 2020.
FAU - Plows, Jasmine F
AU  - Plows JF
AD  - The Liggins Institute, University of Auckland, Auckland, New Zealand.
AD  - Children's Hospital Los Angeles, The Saban Research Institute, Los Angeles, CA, 
      USA.
FAU - Morton-Jones, Jacob
AU  - Morton-Jones J
AD  - The Liggins Institute, University of Auckland, Auckland, New Zealand.
FAU - Bridge-Comer, Pania E
AU  - Bridge-Comer PE
AD  - The Liggins Institute, University of Auckland, Auckland, New Zealand.
FAU - Ponnampalam, Anna
AU  - Ponnampalam A
AD  - The Liggins Institute, University of Auckland, Auckland, New Zealand.
AD  - Department of Physiology, University of Auckland, Auckland, New Zealand.
AD  - Department of Obstetrics and Gynecology, University of Auckland, Auckland, New 
      Zealand.
FAU - Stanley, Joanna L
AU  - Stanley JL
AD  - The Liggins Institute, University of Auckland, Auckland, New Zealand.
FAU - Vickers, Mark H
AU  - Vickers MH
AD  - The Liggins Institute, University of Auckland, Auckland, New Zealand.
FAU - Reynolds, Clare M
AU  - Reynolds CM
AD  - The Liggins Institute, University of Auckland, Auckland, New Zealand.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Nutr
JT  - The Journal of nutrition
JID - 0404243
RN  - 0 (Blood Glucose)
RN  - 0 (Sweetening Agents)
RN  - 0 (Thiazines)
RN  - MA3UYZ6K1H (acetosulfame)
SB  - IM
MH  - Adipocytes/drug effects
MH  - Adipose Tissue/*drug effects
MH  - Animals
MH  - Blood Glucose/drug effects
MH  - Diet
MH  - Female
MH  - Fetus/drug effects
MH  - Gastrointestinal Tract/drug effects/metabolism
MH  - Gene Expression Regulation/drug effects
MH  - Glucose Intolerance/*chemically induced
MH  - Liver/drug effects/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Pregnancy
MH  - *Prenatal Exposure Delayed Effects
MH  - *Sweetening Agents
MH  - Thiazines/administration & dosage/*adverse effects
OTO - NOTNLM
OT  - adipose tissue
OT  - fructose
OT  - glucose intolerance
OT  - non-nutritive sweeteners
OT  - pregnancy
EDAT- 2020/04/23 06:00
MHDA- 2020/11/18 06:00
CRDT- 2020/04/23 06:00
PHST- 2020/01/14 00:00 [received]
PHST- 2020/02/20 00:00 [revised]
PHST- 2020/03/26 00:00 [accepted]
PHST- 2020/04/23 06:00 [pubmed]
PHST- 2020/11/18 06:00 [medline]
PHST- 2020/04/23 06:00 [entrez]
AID - S0022-3166(22)02238-6 [pii]
AID - 10.1093/jn/nxaa106 [doi]
PST - ppublish
SO  - J Nutr. 2020 Jul 1;150(7):1773-1781. doi: 10.1093/jn/nxaa106.