PMID- 32322250
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 11
DP  - 2020
TI  - Occurrence and Diversity of CRISPR Loci in Lactobacillus casei Group.
PG  - 624
LID - 10.3389/fmicb.2020.00624 [doi]
LID - 624
AB  - Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) is an adaptive 
      immune system that resists foreign genes through nuclease targeting in bacteria 
      and archaea. In this study, we analyzed 68 strains of Lactobacillus casei group 
      from the NCBI GenBank database, and bioinformatic tools were used to investigate 
      the occurrence and diversity of CRISPR system. The results showed that a total of 
      30 CRISPR loci were identified from 27 strains. Apart from three strains which 
      contained double loci with distinguishable distributed sites, most strains 
      contained only one CRISPR locus. The analysis of direct repeat (DR) sequences 
      showed that all DR could form stable RNA secondary structures. The CRISPR spacers 
      showed diversity, and their origin and evolution were revealed through the 
      investigation of their spacer sequences. In addition, a large number of CRISPR 
      spacers showed perfect homologies to phage and plasmid sequences. Collectively, 
      our results would contribute to researches of resistance in L. casei group, and 
      also provide a new vision on the diversity and evolution of CRISPR/Cas system.
CI  - Copyright (c) 2020 Yang, Li, Ujiroghene, Yang, Lu, Zhang, Pang and Lv.
FAU - Yang, Lan
AU  - Yang L
AD  - Institute of Food Science and Technology, Chinese Academy of Agricultural 
      Sciences, Beijing, China.
FAU - Li, Weixun
AU  - Li W
AD  - Institute of Food Science and Technology, Chinese Academy of Agricultural 
      Sciences, Beijing, China.
FAU - Ujiroghene, Obaroakpo Joy
AU  - Ujiroghene OJ
AD  - Institute of Food Science and Technology, Chinese Academy of Agricultural 
      Sciences, Beijing, China.
FAU - Yang, Yang
AU  - Yang Y
AD  - Institute of Food Science and Technology, Chinese Academy of Agricultural 
      Sciences, Beijing, China.
FAU - Lu, Jing
AU  - Lu J
AD  - Institute of Food Science and Technology, Chinese Academy of Agricultural 
      Sciences, Beijing, China.
FAU - Zhang, Shuwen
AU  - Zhang S
AD  - Institute of Food Science and Technology, Chinese Academy of Agricultural 
      Sciences, Beijing, China.
FAU - Pang, Xiaoyang
AU  - Pang X
AD  - Institute of Food Science and Technology, Chinese Academy of Agricultural 
      Sciences, Beijing, China.
FAU - Lv, Jiaping
AU  - Lv J
AD  - Institute of Food Science and Technology, Chinese Academy of Agricultural 
      Sciences, Beijing, China.
LA  - eng
PT  - Journal Article
DEP - 20200408
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC7156538
OTO - NOTNLM
OT  - CRISPR system
OT  - Lactobacillus casei group
OT  - genotyping
OT  - phage
OT  - spacer
EDAT- 2020/04/24 06:00
MHDA- 2020/04/24 06:01
PMCR- 2020/04/08
CRDT- 2020/04/24 06:00
PHST- 2019/09/25 00:00 [received]
PHST- 2020/03/19 00:00 [accepted]
PHST- 2020/04/24 06:00 [entrez]
PHST- 2020/04/24 06:00 [pubmed]
PHST- 2020/04/24 06:01 [medline]
PHST- 2020/04/08 00:00 [pmc-release]
AID - 10.3389/fmicb.2020.00624 [doi]
PST - epublish
SO  - Front Microbiol. 2020 Apr 8;11:624. doi: 10.3389/fmicb.2020.00624. eCollection 
      2020.