PMID- 32322545
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240328
IS  - 2215-0161 (Print)
IS  - 2215-0161 (Electronic)
IS  - 2215-0161 (Linking)
VI  - 7
DP  - 2020
TI  - Development of a portable leaf photosynthesis and volatile organic compounds 
      emission system.
PG  - 100880
LID - 10.1016/j.mex.2020.100880 [doi]
LID - 100880
AB  - Understanding how plant carbon metabolism responds to environmental variables 
      such as light is central to understanding ecosystem carbon cycling and the 
      production of food, biofuels, and biomaterials. Here, we couple a portable leaf 
      photosynthesis system to an autosampler for volatile organic compounds (VOCs) to 
      enable field observations of net photosynthesis simultaneously with emissions of 
      VOCs as a function of light. Following sample collection, VOCs are analyzed using 
      automated thermal desorption-gas chromatograph-mass spectrometry (TD-GC-MS). An 
      example is presented from a banana plant in the central Amazon with a focus on 
      the response of photosynthesis and the emissions of eight individual monoterpenes 
      to light intensity. Our observations reveal that banana leaf emissions represent 
      a 1.1 +/- 0.1% loss of photosynthesis by carbon. Monoterpene emissions from 
      banana are dominated by trans-beta-ocimene, which accounts for up to 57% of total 
      monoterpene emissions at high light. We conclude that the developed system is 
      ideal for the identification and quantification of VOC emissions from leaves in 
      parallel with CO2 and water fluxes.The system therefore permits the analysis of 
      biological and environmental sensitivities of carbon metabolism in leaves in 
      remote field locations, resulting in the emission of hydrocarbons to the 
      atmosphere.*A field-portable system is developed for the identification and 
      quantification of VOCs from leaves in parallel with leaf physiological 
      measurements including photosynthesis and transpiration.*The system will enable 
      the characterization of carbon and energy allocation to the biosynthesis and 
      emission of VOCs linked with photosynthesis (e.g. isoprene and monoterpenes) and 
      their biological and environmental sensitivities (e.g. light, temperature, 
      CO(2)).*Allow the development of more accurate mechanistic global VOC emission 
      models linked with photosynthesis, improving our ability to predict how forests 
      will respond to climate change. It is our hope that the presented system will 
      contribute with critical data towards these goals across Earth's diverse tropical 
      forests.
CI  - (c) 2020 The Author(s). Published by Elsevier B.V.
FAU - Jardine, Kolby J
AU  - Jardine KJ
AD  - Earth and Environmental Science Area, Lawrence Berkeley National Laboratory, One 
      Cyclotron Rd, building 84-155, Berkeley, CA 94720, USA.
AD  - National Institute for Amazon Research, Department of Forest Management, Ave. 
      Andre Araujo 2936, Manaus, AM 69.080-97, Brazil.
FAU - Zorzanelli, Raquel F
AU  - Zorzanelli RF
AD  - Federal University of Espirito Santo, Ave. Governador Lindemberg, n degrees  316, Centro, 
      Jeronimo Monteiro, ES 29.550-000, Brazil.
FAU - Gimenez, Bruno O
AU  - Gimenez BO
AD  - National Institute for Amazon Research, Department of Forest Management, Ave. 
      Andre Araujo 2936, Manaus, AM 69.080-97, Brazil.
FAU - Robles, Emily
AU  - Robles E
AD  - Earth and Environmental Science Area, Lawrence Berkeley National Laboratory, One 
      Cyclotron Rd, building 84-155, Berkeley, CA 94720, USA.
AD  - College of Natural Resources, University of California Berkeley, 260 Mulford 
      Hall, Berkeley, CA, 94720, USA.
FAU - de Oliveira Piva, Luani Rosa
AU  - de Oliveira Piva LR
AD  - Federal University of Parana, Department of Forest Sciences, Ave. Prefeito 
      Lothario Meissner 632, Curitiba, PR 80210-170, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20200415
PL  - Netherlands
TA  - MethodsX
JT  - MethodsX
JID - 101639829
PMC - PMC7169044
OTO - NOTNLM
OT  - Banana plant
OT  - Leaf
OT  - Light
OT  - Photosynthesis
OT  - Tropical forest
OT  - VOCs
COIS- The authors declare that they have no known competing financial interests or 
      personal relationships that could have appeared to influence the work reported in 
      this paper.
EDAT- 2020/04/24 06:00
MHDA- 2020/04/24 06:01
PMCR- 2020/04/15
CRDT- 2020/04/24 06:00
PHST- 2020/03/02 00:00 [received]
PHST- 2020/03/23 00:00 [accepted]
PHST- 2020/04/24 06:00 [entrez]
PHST- 2020/04/24 06:00 [pubmed]
PHST- 2020/04/24 06:01 [medline]
PHST- 2020/04/15 00:00 [pmc-release]
AID - S2215-0161(20)30099-6 [pii]
AID - 100880 [pii]
AID - 10.1016/j.mex.2020.100880 [doi]
PST - epublish
SO  - MethodsX. 2020 Apr 15;7:100880. doi: 10.1016/j.mex.2020.100880. eCollection 2020.