PMID- 32323440
OWN - NLM
STAT- MEDLINE
DCOM- 20211103
LR  - 20211103
IS  - 1552-4965 (Electronic)
IS  - 1549-3296 (Linking)
VI  - 108
IP  - 10
DP  - 2020 Oct
TI  - Enhanced chondrogenesis of human umbilical cord mesenchymal stem cells in a 
      gelatin honeycomb scaffold.
PG  - 2069-2079
LID - 10.1002/jbm.a.36966 [doi]
AB  - Transplantation of chondrogenic stem cells is a promising strategy for cartilage 
      repair, but requires improvements in cell sourcing, maintenance, and chondrogenic 
      differentiation efficiency. We examined whether gelatin honeycomb scaffolds can 
      enhance the proliferation, viability, and chondrogenic capability of human 
      umbilical cord mesenchymal stem cells (HUCMSCs) compared to standard plate 
      cultures. In addition, the survival and phenotypic stability of HUCMSC-derived 
      chondrocytes in a scaffold were evaluated in mice over 6 weeks 
      post-transplantation. Survival and proliferation rates of HUCMSCs were comparable 
      between scaffold and plate culture. Scaffold culture in a chondrogenic 
      differentiation medium induced more stable expression of the key hyaline 
      cartilage genes COL2A1 and ACAN and the production of the respective proteins 
      Type II collagen and aggrecan as well as glycosaminoglycan. These 
      HUCMSC-differentiated chondrocytes also stably expressed cartilage genes and 
      proteins in the scaffold 6 weeks after transplantation into nonobese 
      diabetic/severe combined immunodeficient mice. These findings indicate that 
      honeycomb-like gelatin scaffolds can promote the survival and chondrogenic 
      differentiation of HUCMSCs to form hyaline-like cartilage.
CI  - (c) 2020 Wiley Periodicals, Inc.
FAU - Chang, Yu-Hsun
AU  - Chang YH
AD  - Department of Pediatrics, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical 
      Foundation, Tzu Chi University, Hualien, Taiwan.
FAU - Wu, Kun-Chi
AU  - Wu KC
AD  - Department of Orthopedics, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical 
      Foundation, Tzu Chi University, Hualien, Taiwan.
FAU - Wang, Chen-Chie
AU  - Wang CC
AD  - Department of Orthopedics, Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical 
      Foundation, Tzu Chi University, New Taipei City, Taiwan.
FAU - Ding, Dah-Ching
AU  - Ding DC
AUID- ORCID: 0000-0001-5105-068X
AD  - Department of Obstetrics and Gynecology, Hualien Tzu Chi Hospital, Buddhist Tzu 
      Chi Medical Foundation, Tzu Chi University, Hualien, Taiwan.
AD  - Institute of Medical Sciences, Tzu Chi University, Hualien, Taiwan.
AD  - Stem Cell Laboratory, Department of Research, Hualien Tzu Chi Hospital, Buddhist 
      Tzu Chi Medical Foundation, Hualien, Taiwan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200508
PL  - United States
TA  - J Biomed Mater Res A
JT  - Journal of biomedical materials research. Part A
JID - 101234237
RN  - 9000-70-8 (Gelatin)
SB  - IM
MH  - Cells, Cultured
MH  - Chondrocytes/cytology
MH  - *Chondrogenesis
MH  - Gelatin/*chemistry
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology
MH  - Tissue Engineering
MH  - Tissue Scaffolds/*chemistry
MH  - Umbilical Cord/cytology
OTO - NOTNLM
OT  - human umbilical cord mesenchymal stem cells
OT  - osteoarthritis
OT  - scaffold
OT  - stem cell therapy
EDAT- 2020/04/24 06:00
MHDA- 2021/11/04 06:00
CRDT- 2020/04/24 06:00
PHST- 2019/05/08 00:00 [received]
PHST- 2020/03/19 00:00 [revised]
PHST- 2020/03/28 00:00 [accepted]
PHST- 2020/04/24 06:00 [pubmed]
PHST- 2021/11/04 06:00 [medline]
PHST- 2020/04/24 06:00 [entrez]
AID - 10.1002/jbm.a.36966 [doi]
PST - ppublish
SO  - J Biomed Mater Res A. 2020 Oct;108(10):2069-2079. doi: 10.1002/jbm.a.36966. Epub 
      2020 May 8.