PMID- 32323455
OWN - NLM
STAT- MEDLINE
DCOM- 20211027
LR  - 20211027
IS  - 1552-4965 (Electronic)
IS  - 1549-3296 (Linking)
VI  - 108
IP  - 9
DP  - 2020 Sep
TI  - A biphasic, demineralized, and Decellularized allograft bone-hydrogel scaffold 
      with a cell-based BMP-7 delivery system for osteochondral defect regeneration.
PG  - 1909-1921
LID - 10.1002/jbm.a.36954 [doi]
AB  - The reconstruction of bone and cartilage defects remains a challenge in 
      orthopedics and tissue engineering. In this study, a mimetic natural scaffold 
      based on a demineralized and decellularized bone and collagen type I (Col I) 
      allograft was developed. The resulting hydrogel has the capability of loading 
      allogeneic bone marrow mesenchymal stem cells (BMSCs) resulting in the sustained 
      release of bone morphogenetic protein-7 (BMP-7). BMSCs transfected with 
      lentivirus loaded with BMP-7 gene were used as the BMP-7 delivery system, and 
      then seeded on a demineralized and decellularized allograft bone-collagen 
      biphasic scaffold to enhance bone and cartilage regeneration. The results 
      indicated that, after transfection, BMSCs had a higher expression of the BMP-7 
      gene and the sustained release of BMP-7 lasted more than 28 days. The preliminary 
      biphasic scaffold promoted cell adhesion and proliferation. After implanting the 
      transfected cells into the knee joints of beagle dogs, enhanced osteochondral 
      defect regeneration was identified at 12 weeks postimplantation. Our results 
      revealed that the new cell-loaded scaffold can avoid the side effects and the 
      short half-life of BMP-7, and promote the reconstruction of bone and cartilage 
      defects. Such a composite system, therefore, shows potential in bone and 
      cartilage tissue engineering applications.
CI  - (c) 2020 Wiley Periodicals, Inc.
FAU - Sun, Jiachen
AU  - Sun J
AD  - Department of Orthopedics, West China Hospital, Sichuan University, Chengdu, P. 
      R. China.
FAU - Lyu, Jingtong
AU  - Lyu J
AD  - Department of Orthopedics, Southwest Hospital, Third Military Medical University, 
      Chongqing, P. R. China.
FAU - Xing, Fei
AU  - Xing F
AD  - Department of Orthopedics, West China Hospital, Sichuan University, Chengdu, P. 
      R. China.
FAU - Chen, Ran
AU  - Chen R
AD  - Department of Orthopedics, West China Hospital, Sichuan University, Chengdu, P. 
      R. China.
FAU - Duan, Xin
AU  - Duan X
AD  - Department of Orthopedics, West China Hospital, Sichuan University, Chengdu, P. 
      R. China.
FAU - Xiang, Zhou
AU  - Xiang Z
AD  - Department of Orthopedics, West China Hospital, Sichuan University, Chengdu, P. 
      R. China.
AD  - Division of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, 
      West China Hospital, Sichuan University, Chengdu, P. R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200509
PL  - United States
TA  - J Biomed Mater Res A
JT  - Journal of biomedical materials research. Part A
JID - 101234237
RN  - 0 (Bone Morphogenetic Protein 7)
RN  - 0 (Hydrogels)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Bone Morphogenetic Protein 7/*genetics
MH  - Cells, Cultured
MH  - Chondrogenesis
MH  - Collagen/*chemistry
MH  - Dogs
MH  - HEK293 Cells
MH  - Humans
MH  - Hydrogels/*chemistry
MH  - Knee Injuries/therapy
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*cytology
MH  - Osteogenesis
MH  - Tissue Engineering
MH  - Tissue Scaffolds/*chemistry
MH  - Transfection
OTO - NOTNLM
OT  - BMP-7
OT  - bone and cartilage regeneration
OT  - demineralized and decellularized allograft bone
OT  - gene transfection
OT  - sustained release
EDAT- 2020/04/24 06:00
MHDA- 2021/10/28 06:00
CRDT- 2020/04/24 06:00
PHST- 2019/10/15 00:00 [received]
PHST- 2020/03/18 00:00 [revised]
PHST- 2020/03/28 00:00 [accepted]
PHST- 2020/04/24 06:00 [pubmed]
PHST- 2021/10/28 06:00 [medline]
PHST- 2020/04/24 06:00 [entrez]
AID - 10.1002/jbm.a.36954 [doi]
PST - ppublish
SO  - J Biomed Mater Res A. 2020 Sep;108(9):1909-1921. doi: 10.1002/jbm.a.36954. Epub 
      2020 May 9.