PMID- 32325216
OWN - NLM
STAT- MEDLINE
DCOM- 20210115
LR  - 20210115
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 102
DP  - 2020 Jul
TI  - CCR7, CD80/86 and CD83 in yellow catfish (Pelteobagrus fulvidraco): Molecular 
      characteristics and expression patterns with bacterial infection.
PG  - 228-242
LID - S1050-4648(20)30272-2 [pii]
LID - 10.1016/j.fsi.2020.04.026 [doi]
AB  - Dendritic cells (DCs) have a strong ability to stimulate naive T lymphocyte 
      proliferation, so DCs play an important regulatory role in the initiation of the 
      specific immune response. DCs cannot play the role of antigen presentation 
      without the expression of surface molecules. The chemokine receptor CCR7 and the 
      costimulatory molecules CD80/86 and CD83 are not only markers of DC maturation 
      but also important functional molecules in the immune response of DC-T cells. In 
      this study, partial cDNA sequences of CCR7, CD80/86 and CD83 were obtained by 
      rapid amplification of cDNA ends (RACE) technology from yellow catfish. 
      Bioinformatics analysis of deduced amino acid sequences of these three genes 
      showed that CCR7, CD80/86 and CD83 genes in yellow catfish have similar 
      functional domains to the homologs in other vertebrates, which indicated that the 
      functions of these genes may be somewhat conserved during the evolution process. 
      Afterward, the expression characteristics of these three genes in different 
      tissues were detected by q-PCR. This result indicated that CCR7, CD80/86 and CD83 
      were expressed in all examined tissues, and the highest expression levels of CCR7 
      and CD80/86 and CD83 were detected in the trunk kidney, muscle and midgut, 
      respectively. Meanwhile, the expression levels of CCR7 and CD80/86 were lowest in 
      the gill, and the expression of CD83 was lowest in the stomach. Finally, healthy 
      yellow catfish were infected with A.hydrophila (1.0 x 10(7) CFU/mL) or E.ictaluri 
      (1.0 x 10(6) CFU/mL), q-PCR results indicated that both pathogenic bacteria can 
      induce significant upregulation of CCR7, CD80/86 and CD83 in immune organs, and 
      the expression levels of these genes in the intestine were higher than those in 
      the skin and gill. Our results in this study provide a molecular basis for 
      exploring the role of CCR7, CD80/86 and CD83 in the immune responses induced by 
      bacteria, and can help us to understand the difference of immune responses 
      induced by extracellular and intracellular bacteria.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Wang, Hui
AU  - Wang H
AD  - College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China; 
      Hubei Engineering Technology Research Center for Aquatic Animal Diseases Control 
      and Prevention, Wuhan, 430070, China.
FAU - Xu, Lili
AU  - Xu L
AD  - College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China; 
      Hubei Provincial Engineering Laboratory for Pond Aquaculture, Wuhan, 430070, 
      China.
FAU - Wu, Zhixin
AU  - Wu Z
AD  - College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China; 
      Hubei Engineering Technology Research Center for Aquatic Animal Diseases Control 
      and Prevention, Wuhan, 430070, China.
FAU - Chen, Xiaoxuan
AU  - Chen X
AD  - College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China; 
      Hubei Provincial Engineering Laboratory for Pond Aquaculture, Wuhan, 430070, 
      China. Electronic address: chenxx@mail.hzau.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20200420
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (B7 Antigens)
RN  - 0 (Fish Proteins)
RN  - 0 (Receptors, CCR7)
SB  - IM
MH  - Aeromonas hydrophila/physiology
MH  - Amino Acid Sequence
MH  - Animals
MH  - B7 Antigens/chemistry/genetics/immunology
MH  - Base Sequence
MH  - Catfishes
MH  - Edwardsiella ictaluri/physiology
MH  - Enterobacteriaceae Infections/immunology/veterinary
MH  - Fish Diseases/*immunology
MH  - Fish Proteins/chemistry/*genetics/*immunology
MH  - Gene Expression Profiling/veterinary
MH  - Gene Expression Regulation/*immunology
MH  - Gram-Negative Bacterial Infections/immunology/veterinary
MH  - Immunity/*genetics
MH  - Perciformes/*genetics/*immunology
MH  - Phylogeny
MH  - Receptors, CCR7/chemistry/genetics/immunology
MH  - Sequence Alignment/veterinary
OTO - NOTNLM
OT  - Aeromonas hydrophila
OT  - CCR7
OT  - CD80/86
OT  - CD83
OT  - Edwardsiella ictaluri
OT  - Pelteobagrus fulvidraco
EDAT- 2020/04/24 06:00
MHDA- 2021/01/16 06:00
CRDT- 2020/04/24 06:00
PHST- 2019/10/26 00:00 [received]
PHST- 2020/03/25 00:00 [revised]
PHST- 2020/04/13 00:00 [accepted]
PHST- 2020/04/24 06:00 [pubmed]
PHST- 2021/01/16 06:00 [medline]
PHST- 2020/04/24 06:00 [entrez]
AID - S1050-4648(20)30272-2 [pii]
AID - 10.1016/j.fsi.2020.04.026 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2020 Jul;102:228-242. doi: 10.1016/j.fsi.2020.04.026. 
      Epub 2020 Apr 20.