PMID- 32329931
OWN - NLM
STAT- MEDLINE
DCOM- 20210622
LR  - 20231112
IS  - 1098-2825 (Electronic)
IS  - 0887-8013 (Print)
IS  - 0887-8013 (Linking)
VI  - 34
IP  - 8
DP  - 2020 Aug
TI  - A long non-coding RNA OLBC15 promotes triple-negative breast cancer progression 
      via enhancing ZNF326 degradation.
PG  - e23304
LID - 10.1002/jcla.23304 [doi]
LID - e23304
AB  - BACKGROUND: The long non-coding RNAs (lncRNAs) have been involved in various 
      processes, including cancer. However, the function of many lncRNAs is still 
      elusive in triple-negative breast cancer (TNBC). METHODS: LncRNA profiling was 
      used to screen for novel lncRNAs related to TNBC. OLBC15 expression was measured 
      via qRT-PCR. In vitro migration and viability assays were conducted to determine 
      the oncogenic role of OLBC15. Xenograft and metastatic models were performed to 
      further investigate effects in vivo. RNA immunoprecipitation (RIP), mass 
      spectrometry (MS), and fluorescence in situ hybridization (FISH) strategies were 
      designed to identify the interaction between ZNF326 and OLBC15. RESULTS: In the 
      current study, we have identified a novel oncogenic lncRNA termed OLBC15 via 
      lncRNA profiling. OLBC15 is highly expressed especially in triple-negative breast 
      cancer. OLBC15 promoted viability and migration in breast cancer cells. Moreover, 
      OLBC15 could accelerate metastasis and xenograft tumor growth. Mechanistic study 
      suggested that OLBC15 could bind a well-characterized tumor suppressor ZNF326 and 
      OLBC15-ZNF326 interaction resulted in ZNF326 destabilization. OLBC15 induced 
      proteasomal ZNF326 degradation through enhanced ubiquitination. OLBC15 and ZNF326 
      protein expression is also negatively correlated in clinical specimens. 
      CONCLUSIONS: Collectively, OLBC15 may serve as an oncogenic lncRNA to facilitate 
      TNBC progression and a putative target for therapeutic anti-breast cancer 
      intervention.
CI  - (c) 2020 The Authors. Journal of Clinical Laboratory Analysis published by Wiley 
      Periodicals, Inc.
FAU - Deng, Chao
AU  - Deng C
AD  - Department of Comprehensive Ward of Oncology, Chongqing Three Gorges Central 
      Hospital, Chongqing, China.
FAU - Zhang, Bojuan
AU  - Zhang B
AD  - Department of Comprehensive Ward of Oncology, Chongqing Three Gorges Central 
      Hospital, Chongqing, China.
FAU - Zhang, Yao
AU  - Zhang Y
AD  - Department of Comprehensive Ward of Oncology, Chongqing Three Gorges Central 
      Hospital, Chongqing, China.
FAU - Xu, Xiaogang
AU  - Xu X
AD  - Department of Comprehensive Ward of Oncology, Chongqing Three Gorges Central 
      Hospital, Chongqing, China.
FAU - Xiong, Deming
AU  - Xiong D
AD  - Department of Comprehensive Ward of Oncology, Chongqing Three Gorges Central 
      Hospital, Chongqing, China.
FAU - Chen, Xiaoyan
AU  - Chen X
AD  - Department of Comprehensive Ward of Oncology, Chongqing Three Gorges Central 
      Hospital, Chongqing, China.
FAU - Wu, Jiaojiao
AU  - Wu J
AUID- ORCID: 0000-0001-5180-7929
AD  - Department of Respiratory Ward of Radiotherapy, Chongqing Three Gorges Central 
      Hospital, Chongqing, China.
LA  - eng
GR  - zdxk201919/Chongqing Regional Medical Key Discipline Construction Project/
PT  - Journal Article
DEP - 20200424
PL  - United States
TA  - J Clin Lab Anal
JT  - Journal of clinical laboratory analysis
JID - 8801384
RN  - 0 (Carrier Proteins)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (ZNF326 protein, human)
SB  - IM
MH  - Breast/metabolism/pathology
MH  - *Carrier Proteins/genetics/metabolism
MH  - Cell Line, Tumor
MH  - Disease Progression
MH  - Female
MH  - Humans
MH  - *RNA, Long Noncoding/genetics/metabolism
MH  - *Triple Negative Breast Neoplasms/genetics/metabolism/pathology
PMC - PMC7439339
OTO - NOTNLM
OT  - OLBC15
OT  - ZNF326
OT  - breast cancer
OT  - oncogenesis
EDAT- 2020/04/25 06:00
MHDA- 2021/06/23 06:00
PMCR- 2020/04/24
CRDT- 2020/04/25 06:00
PHST- 2020/01/20 00:00 [received]
PHST- 2020/02/21 00:00 [revised]
PHST- 2020/02/23 00:00 [accepted]
PHST- 2020/04/25 06:00 [pubmed]
PHST- 2021/06/23 06:00 [medline]
PHST- 2020/04/25 06:00 [entrez]
PHST- 2020/04/24 00:00 [pmc-release]
AID - JCLA23304 [pii]
AID - 10.1002/jcla.23304 [doi]
PST - ppublish
SO  - J Clin Lab Anal. 2020 Aug;34(8):e23304. doi: 10.1002/jcla.23304. Epub 2020 Apr 
      24.