PMID- 32330363
OWN - NLM
STAT- MEDLINE
DCOM- 20210812
LR  - 20210812
IS  - 1932-7005 (Electronic)
IS  - 1932-6254 (Linking)
VI  - 14
IP  - 6
DP  - 2020 Jun
TI  - Wound healing action of nitric oxide-releasing self-expandable collagen sponge.
PG  - 807-818
LID - 10.1002/term.3046 [doi]
AB  - Mounting evidence showing that local nitric oxide (NO) delivery may significantly 
      improve the wound healing process has stimulated the development of wound 
      dressings capable of releasing NO topically. Herein, we describe the preparation 
      of a self-expandable NO-releasing hydrolyzed collagen sponge (CS), charged with 
      the endogenously found NO donor, S-nitrosoglutathione (GSNO). We show that cold 
      pressed and GSNO-charged CS (CS/GSNO) undergo self-expansion to its original 3D 
      shape upon water absorption to a swelling degree of 2,300 wt%, triggering the 
      release of free NO. Topical application of compressed CS/GSNO on wounds in an 
      animal model showed that exudate absorption by CS/GSNO leads to the release of 
      higher NO doses during the inflammatory phase and progressively lower NO doses at 
      later stages of the healing process. Moreover, treated animals showed significant 
      increase in the mRNA expression levels of monocyte chemoattractant protein-1 
      (MCP-1), murine macrophage marker (F4/80), transforming growth factor beta 
      (TGF-beta), stromal cell-derived factor 1 (SDF-1), insulin-like growth factor-1 
      (IGF-1), nitric oxide synthase(iNOS), and matrix metalloproteinase(MMP-9). 
      Cluster differentiation 31 (CD31), vascular endothelial growth factor (VEGF), and 
      F4/80 were measured on Days 7 and 12 by immunohistochemistry in the cicatricial 
      tissue. These results indicate that the topical delivery of NO enhances the 
      migration and infiltration of leucocytes, macrophages, and keratinocytes to the 
      wounded tissue, as well as the neovascularization and collagen deposition, which 
      are correlated with an accelerated wound closure. Thus, self-expandable CS/GSNO 
      may represent a novel biocompatible and active wound dress for the topical 
      delivery of NO on wounds.
CI  - (c) 2020 John Wiley & Sons, Ltd.
FAU - Povoa, Valeria C O
AU  - Povoa VCO
AUID- ORCID: 0000-0003-4660-2763
AD  - Nursing School, University of Campinas, UNICAMP, Campinas, Brazil.
FAU - Dos Santos, Giovanna J V P
AU  - Dos Santos GJVP
AD  - Institute of Chemistry, University of Campinas, UNICAMP, Campinas, Brazil.
FAU - Picheth, Guilherme F
AU  - Picheth GF
AUID- ORCID: 0000-0002-2802-0180
AD  - Institute of Chemistry, University of Campinas, UNICAMP, Campinas, Brazil.
FAU - Jara, Carlos P
AU  - Jara CP
AUID- ORCID: 0000-0001-5444-721X
AD  - Nursing School, University of Campinas, UNICAMP, Campinas, Brazil.
FAU - da Silva, Laura C E
AU  - da Silva LCE
AUID- ORCID: 0000-0002-9191-3440
AD  - Institute of Chemistry, University of Campinas, UNICAMP, Campinas, Brazil.
FAU - de Araujo, Eliana P
AU  - de Araujo EP
AUID- ORCID: 0000-0002-7539-8477
AD  - Nursing School, University of Campinas, UNICAMP, Campinas, Brazil.
FAU - de Oliveira, Marcelo G
AU  - de Oliveira MG
AUID- ORCID: 0000-0002-9371-9975
AD  - Institute of Chemistry, University of Campinas, UNICAMP, Campinas, Brazil.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200509
PL  - England
TA  - J Tissue Eng Regen Med
JT  - Journal of tissue engineering and regenerative medicine
JID - 101308490
RN  - 0 (Drug Implants)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 57564-91-7 (S-Nitrosoglutathione)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - *Collagen/chemistry/pharmacology
MH  - Disease Models, Animal
MH  - Drug Implants/chemistry/pharmacokinetics/pharmacology
MH  - Male
MH  - Mice
MH  - *Nitric Oxide/chemistry/pharmacokinetics/pharmacology
MH  - *S-Nitrosoglutathione/chemistry/pharmacokinetics/pharmacology
MH  - Wound Healing/*drug effects
MH  - *Wounds and Injuries/drug therapy/metabolism/pathology
OTO - NOTNLM
OT  - S-nitrosoglutathione
OT  - hydrolyzed collagen sponge
OT  - nitric oxide
OT  - topical application
OT  - wound healing
EDAT- 2020/04/25 06:00
MHDA- 2021/08/13 06:00
CRDT- 2020/04/25 06:00
PHST- 2020/02/21 00:00 [received]
PHST- 2020/04/16 00:00 [revised]
PHST- 2020/04/21 00:00 [accepted]
PHST- 2020/04/25 06:00 [pubmed]
PHST- 2021/08/13 06:00 [medline]
PHST- 2020/04/25 06:00 [entrez]
AID - 10.1002/term.3046 [doi]
PST - ppublish
SO  - J Tissue Eng Regen Med. 2020 Jun;14(6):807-818. doi: 10.1002/term.3046. Epub 2020 
      May 9.