PMID- 32331261
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 2223-7747 (Print)
IS  - 2223-7747 (Electronic)
IS  - 2223-7747 (Linking)
VI  - 9
IP  - 4
DP  - 2020 Apr 22
TI  - Insight into the Chromosome Structure of the Cultivated Tetraploid Alfalfa 
      (Medicago sativa subsp. sativa L.) by a Combined Use of GISH and FISH Techniques.
LID - 10.3390/plants9040542 [doi]
LID - 542
AB  - Cytogenetic research in Medicago sativa subsp. sativa L., the cultivated 
      tetraploid alfalfa (2n = 4x = 32), has lagged behind other crops mostly due to 
      the small size and the uniform morphology of its chromosomes. However, in the 
      last decades, the development of molecular cytogenetic techniques based on in 
      situ hybridization has largely contributed to overcoming these limitations. The 
      purpose of this study was to extend our knowledge about the chromosome structure 
      of alfalfa by using a combination of genomic in situ hybridization (GISH) and 
      fluorescence in situ hybridization (FISH) techniques. The results of self-GISH 
      (sGISH) suggested that a substantial part of the repetitive fraction of the 
      genome of subsp. sativa is constituted by tandem repeats typical of satellite 
      DNA. The coincidence of sGISH and C-banding patterns supported this assumption. 
      The FISH mapping of the Arabidopsis-type TTTAGGG telomeric repeats demonstrated, 
      for the first time, that the alfalfa telomeres consist of this type of sequence 
      and revealed a massive presence of interstitial telomeric repeats (ITRs). In the 
      light of this finding M. sativa appears to be a suitable material for studying 
      the origin and function of such extra telomeric repeats. To further exploit this 
      result, investigation will be extended to the diploid subspp. coerulea and 
      falcata in order to explore possible connections between the distribution of 
      ITRs, the ploidy level, and the evolutionary pathway of the taxa.
FAU - Falistocco, Egizia
AU  - Falistocco E
AD  - Department of Agricultural, Food and Environmental Sciences, University of 
      Perugia, Borgo XX Giugno, 06121 Perugia, Italy.
LA  - eng
PT  - Journal Article
DEP - 20200422
PL  - Switzerland
TA  - Plants (Basel)
JT  - Plants (Basel, Switzerland)
JID - 101596181
PMC - PMC7238020
OTO - NOTNLM
OT  - C-banding
OT  - FISH
OT  - GISH
OT  - Medicago sativa
OT  - repetitive DNA
OT  - telomeres
COIS- The author declares no conflicts of interest.
EDAT- 2020/04/26 06:00
MHDA- 2020/04/26 06:01
PMCR- 2020/04/22
CRDT- 2020/04/26 06:00
PHST- 2020/02/14 00:00 [received]
PHST- 2020/03/11 00:00 [revised]
PHST- 2020/03/27 00:00 [accepted]
PHST- 2020/04/26 06:00 [entrez]
PHST- 2020/04/26 06:00 [pubmed]
PHST- 2020/04/26 06:01 [medline]
PHST- 2020/04/22 00:00 [pmc-release]
AID - plants9040542 [pii]
AID - plants-09-00542 [pii]
AID - 10.3390/plants9040542 [doi]
PST - epublish
SO  - Plants (Basel). 2020 Apr 22;9(4):542. doi: 10.3390/plants9040542.