PMID- 32332120
OWN - NLM
STAT- MEDLINE
DCOM- 20201022
LR  - 20201207
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 40
IP  - 23
DP  - 2020 Jun 3
TI  - Spike Afterpotentials Shape the In Vivo Burst Activity of Principal Cells in 
      Medial Entorhinal Cortex.
PG  - 4512-4524
LID - 10.1523/JNEUROSCI.2569-19.2020 [doi]
AB  - Principal neurons in rodent medial entorhinal cortex (MEC) generate 
      high-frequency bursts during natural behavior. While in vitro studies point to 
      potential mechanisms that could support such burst sequences, it remains unclear 
      whether these mechanisms are effective under in vivo conditions. In this study, 
      we focused on the membrane-potential dynamics immediately following action 
      potentials (APs), as measured in whole-cell recordings from male mice running in 
      virtual corridors (Domnisoru et al., 2013). These afterpotentials consisted 
      either of a hyperpolarization, an extended ramp-like shoulder, or a 
      depolarization reminiscent of depolarizing afterpotentials (DAPs) recorded in 
      vitro in MEC principal neurons. Next, we correlated the afterpotentials with the 
      cells' propensity to fire bursts. All DAP cells with known location resided in 
      Layer II, generated bursts, and their interspike intervals (ISIs) were typically 
      between 5 and 15 ms. The ISI distributions of Layer-II cells without DAPs peaked 
      sharply at around 4 ms and varied only minimally across that group. This 
      dichotomy in burst behavior is explained by cell-group-specific DAP dynamics. The 
      same two groups of bursting neurons also emerged when we clustered extracellular 
      spike-train autocorrelations measured in real 2D arenas (Latuske et al., 2015). 
      Apart from slight variations in grid spacing, no difference in the spatial coding 
      properties of the grid cells across all three groups was discernible. Layer III 
      neurons were only sparsely bursting (SB) and had no DAPs. As various mechanisms 
      for modulating ion-channels underlying DAPs exist, our results suggest that 
      temporal features of MEC activity can be altered while maintaining the cells' 
      overall spatial tuning characteristics.SIGNIFICANCE STATEMENT Depolarizing 
      afterpotentials (DAPs) are frequently observed in principal neurons from slice 
      preparations of rodent medial entorhinal cortex (MEC), but their functional role 
      in vivo is unknown. Analyzing whole-cell data from mice running on virtual 
      tracks, we show that DAPs do occur during behavior. Cells with prominent DAPs are 
      found in Layer II; their interspike intervals (ISIs) reflect DAP time-scales. In 
      contrast, neither the rarely bursting cells in Layer III, nor the high-frequency 
      bursters in Layer II, have a DAP. Extracellular recordings from mice exploring 
      real 2D arenas demonstrate that grid cells within these three groups have similar 
      spatial coding properties. We conclude that DAPs shape the temporal response 
      characteristics of principal neurons in MEC with little effect on spatial 
      properties.
CI  - Copyright (c) 2020 the authors.
FAU - Csordas, Dora E
AU  - Csordas DE
AD  - Bernstein Center for Computational Neuroscience Munich and Faculty of Biology, 
      Ludwig-Maximilians-Universitat Munchen, Martinsried-Planegg 82152, Germany.
FAU - Fischer, Caroline
AU  - Fischer C
AD  - Bernstein Center for Computational Neuroscience Munich and Faculty of Biology, 
      Ludwig-Maximilians-Universitat Munchen, Martinsried-Planegg 82152, Germany.
FAU - Nagele, Johannes
AU  - Nagele J
AD  - Bernstein Center for Computational Neuroscience Munich and Faculty of Biology, 
      Ludwig-Maximilians-Universitat Munchen, Martinsried-Planegg 82152, Germany.
FAU - Stemmler, Martin
AU  - Stemmler M
AUID- ORCID: 0000-0002-9040-0475
AD  - Bernstein Center for Computational Neuroscience Munich and Faculty of Biology, 
      Ludwig-Maximilians-Universitat Munchen, Martinsried-Planegg 82152, Germany.
FAU - Herz, Andreas V M
AU  - Herz AVM
AUID- ORCID: 0000-0002-3836-565X
AD  - Bernstein Center for Computational Neuroscience Munich and Faculty of Biology, 
      Ludwig-Maximilians-Universitat Munchen, Martinsried-Planegg 82152, Germany 
      herz@bio.lmu.de.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200424
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
SB  - IM
MH  - Action Potentials/*physiology
MH  - Animals
MH  - Entorhinal Cortex/*cytology/*physiology
MH  - Grid Cells/*physiology
MH  - Male
MH  - Membrane Potentials/physiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Principal Component Analysis/methods
PMC - PMC7275867
OTO - NOTNLM
OT  - bursts
OT  - grid cells
OT  - medial entorhinal cortex
OT  - spatial navigation
OT  - spike afterpotentials
OT  - whole-cell recording in vivo
EDAT- 2020/04/26 06:00
MHDA- 2020/10/23 06:00
PMCR- 2020/12/03
CRDT- 2020/04/26 06:00
PHST- 2019/10/28 00:00 [received]
PHST- 2020/04/03 00:00 [revised]
PHST- 2020/04/11 00:00 [accepted]
PHST- 2020/04/26 06:00 [pubmed]
PHST- 2020/10/23 06:00 [medline]
PHST- 2020/04/26 06:00 [entrez]
PHST- 2020/12/03 00:00 [pmc-release]
AID - JNEUROSCI.2569-19.2020 [pii]
AID - JN-RM-2569-19 [pii]
AID - 10.1523/JNEUROSCI.2569-19.2020 [doi]
PST - ppublish
SO  - J Neurosci. 2020 Jun 3;40(23):4512-4524. doi: 10.1523/JNEUROSCI.2569-19.2020. 
      Epub 2020 Apr 24.