PMID- 32333014
OWN - NLM
STAT- MEDLINE
DCOM- 20210910
LR  - 20210910
IS  - 1529-7268 (Electronic)
IS  - 0006-3363 (Linking)
VI  - 103
IP  - 1
DP  - 2020 Jun 23
TI  - MicroRNA-21 as a regulator of human cumulus cell viability and its potential 
      influence on the developmental potential of the oocyte.
PG  - 94-103
LID - 10.1093/biolre/ioaa058 [doi]
AB  - MicroRNA-21 is expressed in bovine, murine, and human cumulus cells with its 
      expression in murine and bovine cumulus cells correlated with oocyte 
      developmental potential. The aim of this study was to assess the relationship 
      between cumulus cell MIR-21 and human oocyte developmental potential. These 
      studies revealed that both the immature and mature forms of MicroRNA-21 
      (MIR-21-5p) were elevated in cumulus cells of oocytes that developed into 
      blastocysts compared to cumulus cells of oocytes that arrested prior to 
      blastocyst formation. This increase in MicroRNA-21 was observed regardless of 
      whether the oocytes developed into euploid or aneuploid blastocysts. Moreover, 
      MIR-21-5p levels in cumulus cells surrounding oocytes that either failed to 
      mature or matured to metaphase II but failed to fertilize, were  approximately 50% less than 
      the MIR-21-5p levels associated with oocytes that arrested prior to blastocyst 
      formation. Why cumulus cells associated with oocytes of reduced developmental 
      potential expressed less MIR-21-5p is unknown. It is unlikely due to reduced 
      expression of either the receptors of growth differentiation factor 9 or rosha 
      Ribonuclease III (DROSHA) and Dicer Ribonuclease III (DICER) which sequentially 
      promote the conversion of immature forms of MicroRNA-21 to mature MicroRNA-21. 
      Furthermore, cultured cumulus cells treated with a MIR-21-5p inhibitor had an 
      increase in apoptosis and a corresponding increase in the expression of PTEN, a 
      gene known to inhibit the AKT-dependent survival pathway in cumulus cells. These 
      studies provide evidence for a role of MicroRNA-21 in human cumulus cells that 
      influences the developmental potential of human oocytes.
CI  - (c) The Author(s) 2020. Published by Oxford University Press on behalf of Society 
      for the Study of Reproduction. All rights reserved. For permissions, please 
      e-mail: journals.permissions@oup.com.
FAU - Bartolucci, Alison F
AU  - Bartolucci AF
AD  - Department of Obstetrics and Gynecology, Farmington, CT 06030, USA.
AD  - The Center for Advanced Reproductive Services, University of Connecticut Health 
      Center, Farmington, CT 06030, USA.
FAU - Uliasz, Tracy
AU  - Uliasz T
AD  - Department of Cell Biology, University of CT Health Center, Farmington, CT 06030, 
      USA.
FAU - Peluso, John J
AU  - Peluso JJ
AD  - Department of Obstetrics and Gynecology, Farmington, CT 06030, USA.
AD  - Department of Cell Biology, University of CT Health Center, Farmington, CT 06030, 
      USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (MIRN21 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - EC 3.1.3.67 (PTEN Phosphohydrolase)
RN  - EC 3.1.3.67 (PTEN protein, human)
SB  - IM
MH  - Adult
MH  - Apoptosis/drug effects
MH  - Blastocyst/physiology
MH  - Cell Survival/physiology
MH  - Cells, Cultured
MH  - Cumulus Cells/drug effects/*physiology
MH  - Embryo Culture Techniques
MH  - Female
MH  - Gene Expression
MH  - Humans
MH  - MicroRNAs/antagonists & inhibitors/genetics/*physiology
MH  - Oocytes/*growth & development
MH  - PTEN Phosphohydrolase/genetics
MH  - Sperm Injections, Intracytoplasmic
OTO - NOTNLM
OT  - apoptosis
OT  - blastocyst
OT  - oocyte-follicle interactions
EDAT- 2020/04/26 06:00
MHDA- 2021/09/11 06:00
CRDT- 2020/04/26 06:00
PHST- 2020/01/21 00:00 [received]
PHST- 2020/03/18 00:00 [revised]
PHST- 2020/04/22 00:00 [accepted]
PHST- 2020/04/26 06:00 [pubmed]
PHST- 2021/09/11 06:00 [medline]
PHST- 2020/04/26 06:00 [entrez]
AID - 5824829 [pii]
AID - 10.1093/biolre/ioaa058 [doi]
PST - ppublish
SO  - Biol Reprod. 2020 Jun 23;103(1):94-103. doi: 10.1093/biolre/ioaa058.