PMID- 32333528 OWN - NLM STAT- MEDLINE DCOM- 20200625 LR - 20200625 IS - 1000-0607 (Print) IS - 1000-0607 (Linking) VI - 45 IP - 4 DP - 2020 Apr 25 TI - [Effect of moxibustion on cardiac function and expression of autophagy-related proteins of cardiomyocytes in chronic heart failure rats]. PG - 259-63 LID - 10.13702/j.1000-0607.190138 [doi] AB - OBJECTIVE: To observe the effect of moxibustion on cardiac function and expression of myocardial tumor suppressor protein p53, mammalian target of rapamycin (mTOR) and phosphorylated(p)-mTOR (excessive autophagy-associated proteins of cardiomyocytes) in rats with chronic heart failure (CHF), so as to explore its mechanisms underlying improvement of CHF. METHODS: SD rats were divided into blank control (n=11), model(n=8), autophagy activator (n=8), autophagy inhibitor (n=9) and moxibustion(n=9) groups. The CHF model was established by i.p. injection of Doxorubicin Hydrochloride (DOX, 1 mg/mL, 1-4 mg/kg) every other day. Moxibustion was applied to bilateral "Feishu" (BL13) and "Xinshu" (BL15) for 20 min, 5 times a week for 3 weeks. Rats of the autophagy activator group received gavage of Rapamycin (RAPA, 2 mg/kg) and those of the autophagy inhibitor group received i.p. injection of Methyladenine (3-MA, 15 mg/kg) 5 times a week for 3 weeks after successful modeling. The heart weight and body weight were measured to calculate heart mass index (HW/BW=heart weight / body weight). Cardiac output (CO) and heart rate (HR) were measured by using a cardiac function meter. Serum N-terminal pro-brain natriuretic peptide (NT-pro BNP) content was assayed by using ELISA, and the expression of myocardial p53, p-mTOR and mTOR proteins was examined by Western blot. RESULTS: (1) Compared with the blank control group, the HR, HW/BW, NT-pro BNP content and p53 expression levels were significantly increased (P<0.01), and the CO and ratio of p-mTOR/mTOR were significantly decreased in the model group (P<0.01). (2) Compared with the model group, the HR, HW/BW and NT-pro BNP content of the autophagy inhibitor and moxibustion groups were significantly decreased (P<0.01, P<0.05), and CO and p-mTOR/mTOR ratio were significantly increased in both autophagy inhibitor and moxibustion groups (P<0.01). (3) Compared with the autophagy activator group, the levels of HR, HW/BW, NT-pro BNP and p53 in the autophagy inhibitor and moxibustion groups were significantly lower (P<0.01), and those of CO and p-mTOR/mTOR levels were significantly higher (P<0.01). CONCLUSION: Moxibustion, similar to the autophagy inhibitor, has a protective action on myocardium in CHF rats, which is possible by preventing over expression of myocardial autophagy-associated proteins during CHF. FAU - Li, Qing-Ling AU - Li QL AD - Graduate School, Anhui University of Chinese Medicine, Hefei 230012, China. FAU - Ma, Qiang AU - Ma Q AD - Graduate School, Anhui University of Chinese Medicine, Hefei 230012, China. FAU - Li, Yue AU - Li Y AD - Graduate School, Anhui University of Chinese Medicine, Hefei 230012, China. FAU - Liu, Cong-Ying AU - Liu CY AD - Graduate School, Anhui University of Chinese Medicine, Hefei 230012, China. FAU - Xin, Qing-Ling AU - Xin QL AD - Graduate School, Anhui University of Chinese Medicine, Hefei 230012, China. FAU - Xu, Wei-Nan AU - Xu WN AD - Graduate School, Anhui University of Chinese Medicine, Hefei 230012, China. FAU - Jia, Xue-Zhao AU - Jia XZ AD - Clinical College of Chinese Medicine, Anhui University of Chinese Medicine, Hefei 230012, China. FAU - Wang, Jing AU - Wang J AD - Clinical College of Chinese Medicine, Anhui University of Chinese Medicine, Hefei 230012, China. LA - chi PT - Journal Article PL - China TA - Zhen Ci Yan Jiu JT - Zhen ci yan jiu = Acupuncture research JID - 8507710 RN - 0 (Autophagy-Related Proteins) SB - IM MH - Animals MH - Autophagy-Related Proteins MH - Chronic Disease MH - *Heart Failure MH - *Moxibustion MH - Myocytes, Cardiac MH - Rats MH - Rats, Sprague-Dawley OTO - NOTNLM OT - Cardiac function OT - Chronic heart failure OT - Mammalian target of rapamycin OT - Moxibustion OT - Myocardial autophagy OT - Myocardial tumor suppressor protein p53 EDAT- 2020/04/26 06:00 MHDA- 2020/06/26 06:00 CRDT- 2020/04/26 06:00 PHST- 2020/04/26 06:00 [entrez] PHST- 2020/04/26 06:00 [pubmed] PHST- 2020/06/26 06:00 [medline] AID - 10.13702/j.1000-0607.190138 [doi] PST - ppublish SO - Zhen Ci Yan Jiu. 2020 Apr 25;45(4):259-63. doi: 10.13702/j.1000-0607.190138.