PMID- 32335278
OWN - NLM
STAT- MEDLINE
DCOM- 20210419
LR  - 20210419
IS  - 1872-7913 (Electronic)
IS  - 0924-8579 (Linking)
VI  - 56
IP  - 1
DP  - 2020 Jul
TI  - Synergistic phage-surfactant combination clears IgE-promoted Staphylococcus 
      aureus aggregation in vitro and enhances the effect in vivo.
PG  - 105997
LID - S0924-8579(20)30155-2 [pii]
LID - 10.1016/j.ijantimicag.2020.105997 [doi]
AB  - Currently, topical antibiotic treatment is a major strategy for decolonisation of 
      Staphylococcus aureus, although it may result in antibiotic resistance or 
      recolonisation of the organism. Recently, application of bacteriophages in the 
      treatment of S. aureus infection has attracted attention. However, a single 
      administration of bacteriophages did not effectively decolonise S. aureus in our 
      first trial in vivo. Using a bacteriophage (pSa-3) and surfactant combination in 
      vitro, we showed an increased (>8%) adsorption rate of the bacteriophage on the 
      host. Moreover, the combination increased the eradication of immunoglobulin E 
      (IgE)-stimulated aggregation, as the surfactant promoted the dissociation of S. 
      aureus aggregates by decreasing the size by 75% and 50% in the absence and 
      presence of IgE, respectively. Furthermore, the combined treatment significantly 
      decolonised the pathogen with an efficacy double that of the phage-only 
      treatment, and decreased the expression of pro-inflammatory cytokine genes 
      (IL-1beta, IL-12 and IFNgamma) for 5 days in the second in vivo trial. These results 
      suggest that the bacteriophage-surfactant combination could act as an alternative 
      to antibiotics for S. aureus decolonisation in patients with dermatitis.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Kim, Sang Guen
AU  - Kim SG
AD  - Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research 
      Institute for Veterinary Science, Seoul National University, Seoul, Republic of 
      Korea.
FAU - Giri, Sib Sankar
AU  - Giri SS
AD  - Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research 
      Institute for Veterinary Science, Seoul National University, Seoul, Republic of 
      Korea.
FAU - Yun, Saekil
AU  - Yun S
AD  - Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research 
      Institute for Veterinary Science, Seoul National University, Seoul, Republic of 
      Korea.
FAU - Kim, Hyoun Joong
AU  - Kim HJ
AD  - Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research 
      Institute for Veterinary Science, Seoul National University, Seoul, Republic of 
      Korea.
FAU - Kim, Sang Wha
AU  - Kim SW
AD  - Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research 
      Institute for Veterinary Science, Seoul National University, Seoul, Republic of 
      Korea.
FAU - Kang, Jeong Woo
AU  - Kang JW
AD  - Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research 
      Institute for Veterinary Science, Seoul National University, Seoul, Republic of 
      Korea.
FAU - Han, Se Jin
AU  - Han SJ
AD  - Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research 
      Institute for Veterinary Science, Seoul National University, Seoul, Republic of 
      Korea.
FAU - Kwon, Jun
AU  - Kwon J
AD  - Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research 
      Institute for Veterinary Science, Seoul National University, Seoul, Republic of 
      Korea.
FAU - Oh, Woo Taek
AU  - Oh WT
AD  - Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research 
      Institute for Veterinary Science, Seoul National University, Seoul, Republic of 
      Korea.
FAU - Jun, Jin Woo
AU  - Jun JW
AD  - Department of Aquaculture, Korea National College of Agriculture and Fisheries, 
      Jeonju-si, Republic of Korea.
FAU - Park, Se Chang
AU  - Park SC
AD  - Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research 
      Institute for Veterinary Science, Seoul National University, Seoul, Republic of 
      Korea. Electronic address: parksec@snu.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20200423
PL  - Netherlands
TA  - Int J Antimicrob Agents
JT  - International journal of antimicrobial agents
JID - 9111860
RN  - 0 (Interleukin-1beta)
RN  - 0 (Surface-Active Agents)
RN  - 187348-17-0 (Interleukin-12)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Bacterial Adhesion/*drug effects
MH  - Bacteriophages/*metabolism
MH  - Dermatitis, Atopic/*drug therapy/microbiology
MH  - Humans
MH  - Immunoglobulin E/immunology
MH  - Interferon-gamma/biosynthesis
MH  - Interleukin-12/biosynthesis
MH  - Interleukin-1beta/biosynthesis
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Phage Therapy/*methods
MH  - Staphylococcal Skin Infections/*drug therapy
MH  - Staphylococcus aureus/drug effects/immunology/virology
MH  - Surface-Active Agents/*pharmacology
OTO - NOTNLM
OT  - Aggregation
OT  - Bacteriophage
OT  - Staphylococcus aureus
OT  - Surfactant
OT  - Synergy
OT  - Topical treatment
EDAT- 2020/04/27 06:00
MHDA- 2021/04/20 06:00
CRDT- 2020/04/27 06:00
PHST- 2019/10/15 00:00 [received]
PHST- 2020/04/15 00:00 [revised]
PHST- 2020/04/17 00:00 [accepted]
PHST- 2020/04/27 06:00 [pubmed]
PHST- 2021/04/20 06:00 [medline]
PHST- 2020/04/27 06:00 [entrez]
AID - S0924-8579(20)30155-2 [pii]
AID - 10.1016/j.ijantimicag.2020.105997 [doi]
PST - ppublish
SO  - Int J Antimicrob Agents. 2020 Jul;56(1):105997. doi: 
      10.1016/j.ijantimicag.2020.105997. Epub 2020 Apr 23.