PMID- 32337276
OWN - NLM
STAT- MEDLINE
DCOM- 20210119
LR  - 20210119
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2020
DP  - 2020
TI  - Repairing and Analgesic Effects of Umbilical Cord Mesenchymal Stem Cell 
      Transplantation in Mice with Spinal Cord Injury.
PG  - 7650354
LID - 10.1155/2020/7650354 [doi]
LID - 7650354
AB  - Transplantation of human umbilical cord mesenchymal stem cells (hUC-MSCs) into 
      spinal cord injury (SCI) may alleviate neuropathic pain and promote functional 
      recovery. The underlying mechanism likely involves activation of glial cells and 
      regulation of inflammatory factors but requires further validation. SCI was 
      induced in 16 ICR mice using an SCI compression model, followed by injection of 
      lentiviral vector-mediated green fluorescent protein- (GFP-) labeled hUC-MSCs 1 
      week later. Behavioral tests, histological evaluation, and inflammatory factor 
      detection were performed in the treatment (SCI+hUC-MSCs) and model (SCI) groups. 
      Histological evaluation revealed GFP expression in the spinal cord tissue of the 
      treatment group, implying that the injected MSCs successfully migrated to the 
      SCI. The Basso, Beattie, and Bresnahan (BBB) scores showed that motor function 
      gradually recovered over time in both groups, but recovery speed was 
      significantly higher in the treatment group than in the model group. The pain 
      threshold in mice decreased after SCI but gradually increased over time owing to 
      the self-repair function of the body. The corresponding pain threshold of the 
      treatment group was significantly higher than that of the model group, indicating 
      the therapeutic and analgesic effects of hUC-MSCs. Expression of IL-6 and TNF-alpha 
      in the spinal cord tissue of the treated group decreased, whereas glial cell 
      line-derived neurotrophic factor (GDNF) expression along with ED1 expression 
      increased compared with those in the model group, suggesting that SCI activated 
      ED1 inflammatory macrophages/microglia, which were subsequently reduced by 
      hUC-MSC transplantation. hUC-MSCs are speculated to enhance the repair of the 
      injured spinal cord tissue and exert an analgesic effect by reducing the 
      secretion of inflammatory factors IL-6 and TNF-alpha and upregulating the expression 
      of GDNF.
CI  - Copyright (c) 2020 Ling-ling Wu et al.
FAU - Wu, Ling-Ling
AU  - Wu LL
AD  - Medical Molecular Biology Laboratory, School of Medicine, Jinhua Polytechnic, 
      Jinhua 321007, China.
FAU - Pan, Xiao-Ming
AU  - Pan XM
AD  - Medical Molecular Biology Laboratory, School of Medicine, Jinhua Polytechnic, 
      Jinhua 321007, China.
FAU - Chen, Hao-Hao
AU  - Chen HH
AUID- ORCID: 0000-0001-5496-7944
AD  - Medical Molecular Biology Laboratory, School of Medicine, Jinhua Polytechnic, 
      Jinhua 321007, China.
FAU - Fu, Xiao-Yan
AU  - Fu XY
AD  - Medical Molecular Biology Laboratory, School of Medicine, Jinhua Polytechnic, 
      Jinhua 321007, China.
FAU - Jiang, Jinzhan
AU  - Jiang J
AUID- ORCID: 0000-0003-0255-3373
AD  - Medical Molecular Biology Laboratory, School of Medicine, Jinhua Polytechnic, 
      Jinhua 321007, China.
AD  - Jinhua Center of Laboratory Animals, Jinhua Municipal Food and Drug Inspection 
      Institute, Jinhua, 321000 Zhejiang Province, China.
FAU - Ding, Ming-Xing
AU  - Ding MX
AUID- ORCID: 0000-0001-5859-5770
AD  - Medical Molecular Biology Laboratory, School of Medicine, Jinhua Polytechnic, 
      Jinhua 321007, China.
LA  - eng
PT  - Journal Article
DEP - 20200404
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (Analgesics)
RN  - 0 (Ectodysplasins)
RN  - 0 (Gdnf protein, mouse)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
RN  - 0 (Interleukin-6)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Analgesics/*pharmacology
MH  - Animals
MH  - Ectodysplasins/metabolism
MH  - Glial Cell Line-Derived Neurotrophic Factor/metabolism
MH  - Interleukin-6/metabolism
MH  - Male
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/cytology
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Recovery of Function/physiology
MH  - Spinal Cord/metabolism
MH  - Spinal Cord Injuries/physiopathology/*therapy
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Umbilical Cord/*cytology
PMC - PMC7165320
COIS- The authors declare that they have no conflict of interest.
EDAT- 2020/04/28 06:00
MHDA- 2021/01/20 06:00
PMCR- 2020/04/04
CRDT- 2020/04/28 06:00
PHST- 2019/04/10 00:00 [received]
PHST- 2019/07/02 00:00 [revised]
PHST- 2020/02/10 00:00 [accepted]
PHST- 2020/04/28 06:00 [entrez]
PHST- 2020/04/28 06:00 [pubmed]
PHST- 2021/01/20 06:00 [medline]
PHST- 2020/04/04 00:00 [pmc-release]
AID - 10.1155/2020/7650354 [doi]
PST - epublish
SO  - Biomed Res Int. 2020 Apr 4;2020:7650354. doi: 10.1155/2020/7650354. eCollection 
      2020.