PMID- 32339349
OWN - NLM
STAT- MEDLINE
DCOM- 20210329
LR  - 20210329
IS  - 1095-8355 (Electronic)
IS  - 1065-6995 (Linking)
VI  - 44
IP  - 8
DP  - 2020 Aug
TI  - A comparative study of hBM-MSCs' differentiation toward osteogenic lineage in the 
      presence of progesterone and estrogen hormones separately and concurrently in 
      vitro.
PG  - 1701-1713
LID - 10.1002/cbin.11364 [doi]
AB  - Promising cell sources for tissue engineering comprise bone marrow 
      derived-mesenchymal stem cells (BM-MSCs) that have multiple differentiation 
      potentials. Also, sex hormones act as important elements in bone development and 
      maintenance, and the roles of two female sex steroid hormones known as estrogen 
      (17-beta estradiol) and progesterone in osteogenic differentiation of human BM-MSCs 
      (hBM-MSCs) are studied. For this purpose, hBM-MSCs were treated with a 1 x 10(-6) 
       M concentration of 17-beta estradiol and progesterone separately and simultaneously 
      while the optimum concentrations were obtained by 
      3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. 
      Osteogenic differentiation tests including measurement of alkaline phosphatase 
      (ALP) enzyme activity, the content of total mineral calcium, mineralized matrix 
      staining by Alizarin Red and Von Kossa solutions, real-time reverse transcription 
      polymerase chain reaction (RT-PCR), and immunofluorescence staining were carried 
      out on Days 7 and 14 of differentiation. To exhibit the morphology of the cells, 
      the BM-MSCs were stained with acridine orange (AO) solution. In this study, the 
      results of ALP activity assay, calcium content and real-time RT-PCR assay and 
      also all tests of differentiation staining have shown that 17-beta estradiol has 
      been recognized as an enhancing factor of osteogenic differentiation. 
      Furthermore, MTT assay and AO staining revealed progesterone as a factor that 
      seriously improved the proliferation of hBM-MSCs. Generally, the 17-beta estradiol 
      individually or in the presence of progesterone has more effects on BM-MSCs' 
      osteogenic differentiation compared to progesterone alone. In this study, it is 
      indicated that the effect of the 17-beta estradiol and progesterone concurrently was 
      the same as individual 17-beta estradiol on the differentiation of hBM-MSCs.
CI  - (c) 2020 International Federation for Cell Biology.
FAU - Soltanyzadeh, Maryam
AU  - Soltanyzadeh M
AD  - Department of Cell and Molecular Biology, Faculty of Biological Sciences, 
      Kharazmi University, Tehran, Iran.
FAU - Ghollasi, Marzieh
AU  - Ghollasi M
AD  - Department of Cell and Molecular Biology, Faculty of Biological Sciences, 
      Kharazmi University, Tehran, Iran.
FAU - Halabian, Raheleh
AU  - Halabian R
AUID- ORCID: 0000-0002-3363-8276
AD  - Applied Microbiology Research Center, Systems Biology and Poisonings Institute, 
      Baqiyatallah University of Medical Sciences, Tehran, Iran.
FAU - Shams, Mehdi
AU  - Shams M
AD  - Department of Nanotechnology and Advanced Materials, Materials and Energy 
      Research Center, Karaj, Iran.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20200506
PL  - England
TA  - Cell Biol Int
JT  - Cell biology international
JID - 9307129
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Bone Marrow Cells/cytology
MH  - Cell Lineage
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Estradiol/*pharmacology
MH  - Female
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology/drug effects
MH  - Osteogenesis/*drug effects
MH  - Progesterone/*pharmacology
OTO - NOTNLM
OT  - estrogen
OT  - mesenchymal stem cells
OT  - osteogenic differentiation
OT  - progesterone
EDAT- 2020/04/28 06:00
MHDA- 2021/03/30 06:00
CRDT- 2020/04/28 06:00
PHST- 2019/12/18 00:00 [received]
PHST- 2020/04/12 00:00 [revised]
PHST- 2020/04/25 00:00 [accepted]
PHST- 2020/04/28 06:00 [pubmed]
PHST- 2021/03/30 06:00 [medline]
PHST- 2020/04/28 06:00 [entrez]
AID - 10.1002/cbin.11364 [doi]
PST - ppublish
SO  - Cell Biol Int. 2020 Aug;44(8):1701-1713. doi: 10.1002/cbin.11364. Epub 2020 May 
      6.