PMID- 32339963
OWN - NLM
STAT- MEDLINE
DCOM- 20210329
LR  - 20210329
IS  - 1879-3231 (Electronic)
IS  - 0093-691X (Linking)
VI  - 152
DP  - 2020 Aug
TI  - Kisspeptin/GnRH1 system in Leydig cells of horse (Equus caballus): Presence and 
      function.
PG  - 1-7
LID - S0093-691X(20)30225-9 [pii]
LID - 10.1016/j.theriogenology.2020.04.006 [doi]
AB  - The objectives of this study were to evaluate in horse testes the expression of 
      kisspeptin (KiSS) and GnRH1 neuropeptides and their cognate receptors, KiSS1R and 
      GnRH1R, as well as their action on testosterone, GnRH1, prostaglandin F2alpha 
      (PGF2alpha), and PGE2 synthesis and cyclooxygenase 1 (COX1) and COX2 activity by 
      Leydig cells in vitro. Testes were obtained from 9 sexually mature horses by 
      surgical castration. Immunohistochemistry, evidenced the presence of KiSS, 
      KiSS1R, GnRH, and GnRH1R in Leydig cells, whereas germinal and Sertoli cells were 
      positive only for GnRH1. Transcripts for both neuropeptides and their cognate 
      receptors were revealed in isolated Leydig cells by RT-PCR. Isolated and purified 
      Leydig cells were in vitro cultured with agonists and antagonists of KiSS 
      (KiSS-10 and KiSS-234, respectively) and GnRH1 (buserelin and antide, 
      respectively). KiSS-10 and buserelin increased (P < 0.01) COX1 activity and 
      testosterone and PGF2alpha basal secretion, while decreased (P < 0.01) that of PGE2. 
      KiSS-10 and buserelin did not affect COX2 activity. GnRH1 basal production was 
      increased (P < 0.01) by KiSS-10, but not by buserelin. Antide counteracted the 
      KiSS and GnRH1 effects, whereas KiSS-234 influence only those of KiSS. 
      Summarizing, the KiSS/GnRH1 system is present in horse Leydig cells and modulates 
      their endocrine activity. In particular, the endocrine effects of KiSS are 
      mediated by GnRH1, so suggesting that hypothalamic-like interaction between KiSS 
      and GnRH1 occurs also in Leydig cells.
CI  - Copyright (c) 2020. Published by Elsevier Inc.
FAU - Petrucci, Linda
AU  - Petrucci L
AD  - Dipartimento di Medicina Veterinaria, Universita di Perugia, 06126, Perugia, PG, 
      Italy; Scuola di Bioscienze e Medicina Veterinaria, Universita di Camerino, 
      62032, Camerino, MC, Italy.
FAU - Maranesi, Margherita
AU  - Maranesi M
AD  - Dipartimento di Medicina Veterinaria, Universita di Perugia, 06126, Perugia, PG, 
      Italy. Electronic address: massimo.zerani@unipg.it.
FAU - Verini Supplizi, Andrea
AU  - Verini Supplizi A
AD  - Dipartimento di Medicina Veterinaria, Universita di Perugia, 06126, Perugia, PG, 
      Italy.
FAU - Dall'Aglio, Cecilia
AU  - Dall'Aglio C
AD  - Dipartimento di Medicina Veterinaria, Universita di Perugia, 06126, Perugia, PG, 
      Italy.
FAU - Mandara, Maria Teresa
AU  - Mandara MT
AD  - Dipartimento di Medicina Veterinaria, Universita di Perugia, 06126, Perugia, PG, 
      Italy.
FAU - Quassinti, Luana
AU  - Quassinti L
AD  - Scuola di Scienze del Farmaco e dei Prodotti della Salute, Universita di 
      Camerino, 62032, Camerino, MC, Italy.
FAU - Bramucci, Massimo
AU  - Bramucci M
AD  - Scuola di Scienze del Farmaco e dei Prodotti della Salute, Universita di 
      Camerino, 62032, Camerino, MC, Italy.
FAU - Miano, Antonino
AU  - Miano A
AD  - Scuola di Bioscienze e Medicina Veterinaria, Universita di Camerino, 62032, 
      Camerino, MC, Italy.
FAU - Gobbetti, Anna
AU  - Gobbetti A
AD  - Scuola di Bioscienze e Medicina Veterinaria, Universita di Camerino, 62032, 
      Camerino, MC, Italy.
FAU - Catone, Giuseppe
AU  - Catone G
AD  - Scuola di Bioscienze e Medicina Veterinaria, Universita di Camerino, 62032, 
      Camerino, MC, Italy; Dipartimento di Scienze Veterinarie, Universita di Messina, 
      98122, Messina, ME, Italy.
FAU - Boiti, Cristiano
AU  - Boiti C
AD  - Dipartimento di Medicina Veterinaria, Universita di Perugia, 06126, Perugia, PG, 
      Italy.
FAU - Zerani, Massimo
AU  - Zerani M
AD  - Dipartimento di Medicina Veterinaria, Universita di Perugia, 06126, Perugia, PG, 
      Italy; Scuola di Bioscienze e Medicina Veterinaria, Universita di Camerino, 
      62032, Camerino, MC, Italy.
LA  - eng
PT  - Journal Article
DEP - 20200415
PL  - United States
TA  - Theriogenology
JT  - Theriogenology
JID - 0421510
RN  - 0 (Kisspeptins)
RN  - 0 (Receptors, Kisspeptin-1)
RN  - 0 (Receptors, LHRH)
RN  - 33515-09-2 (Gonadotropin-Releasing Hormone)
RN  - EC 1.14.99.1 (Cyclooxygenase 1)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
SB  - IM
MH  - Animals
MH  - Cyclooxygenase 1/genetics/metabolism
MH  - Cyclooxygenase 2/genetics/metabolism
MH  - Gene Expression Regulation
MH  - Gonadotropin-Releasing Hormone/genetics/*metabolism
MH  - *Horses
MH  - Kisspeptins/genetics/*metabolism
MH  - Leydig Cells/*metabolism
MH  - Male
MH  - Receptors, Kisspeptin-1/genetics/*metabolism
MH  - Receptors, LHRH/genetics/*metabolism
OTO - NOTNLM
OT  - GnRH
OT  - Horse
OT  - Kisspeptin
OT  - Leydig cell
OT  - Prostaglandins
OT  - Testosterone
EDAT- 2020/04/28 06:00
MHDA- 2021/03/30 06:00
CRDT- 2020/04/28 06:00
PHST- 2019/05/22 00:00 [received]
PHST- 2020/04/07 00:00 [revised]
PHST- 2020/04/07 00:00 [accepted]
PHST- 2020/04/28 06:00 [pubmed]
PHST- 2021/03/30 06:00 [medline]
PHST- 2020/04/28 06:00 [entrez]
AID - S0093-691X(20)30225-9 [pii]
AID - 10.1016/j.theriogenology.2020.04.006 [doi]
PST - ppublish
SO  - Theriogenology. 2020 Aug;152:1-7. doi: 10.1016/j.theriogenology.2020.04.006. Epub 
      2020 Apr 15.