PMID- 32340401
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 2072-666X (Print)
IS  - 2072-666X (Electronic)
IS  - 2072-666X (Linking)
VI  - 11
IP  - 4
DP  - 2020 Apr 23
TI  - Survival Rate of Cells Sent by a Low Mechanical Load Tube Pump: The "Ring Pump".
LID - 10.3390/mi11040447 [doi]
LID - 447
AB  - A ring pump (RP) is a useful tool for microchannels and automated cell culturing. 
      We have been developing RPs (a full-press ring pump, FRP; and a mid-press ring 
      pump, MRP). However, damage to cells which were sent by the RP and the MRP was 
      not investigated, and no other studies have compared the damage to cells between 
      RPs and peristaltic pumps (PPs). Therefore, first, we evaluated the damage to 
      cells that were sent by a small size FRP (s-FRP) and small size MRPs (s-MRPs; gap 
      = 25 or 50 mum, respectively). "Small size" means that the s-FRP and the s-MRPs 
      are suitable for microchannel-scale applications. The survival rate of cells sent 
      by the s-MRPs was higher than those sent by the s-FRP, and less damage caused by 
      the former. Second, we compared the survival rate of cells that were sent by a 
      large size FRP (l-FRP), a large size MRP (l-MRP) (gap = 50 mum) and a PP. "Large 
      size" means that the l-FRP and the l-MRP are suitable for automated cell culture 
      system applications. We could not confirm any differences among the cell survival 
      rates. On the other hand, when cells suspended in Dulbecco's phosphate-buffered 
      saline solution were circulated with the l-MRP (gap = 50 mum) and the PP, we 
      confirmed a difference in cell survival rate, and less damage caused by the 
      former.
FAU - Uesugi, Kaoru
AU  - Uesugi K
AD  - Department of Mechanical Engineering, Osaka University, 2-1 Yamada-oka, Suita, 
      Osaka 565-0871, Japan.
AD  - Global Center for Medical Engineering and Informatics, Osaka University, 2-1 
      Yamada-oka Suita, Osaka 565-0871, Japan.
AD  - Department of Mechanical Systems Engineering, Ibaraki University, 4-12-1 
      Nakanarusawacho, Hitachi, Ibaraki 316-8511, Japan.
FAU - Nishiyama, Keizo
AU  - Nishiyama K
AD  - Aquatech Co., Ltd., 2-1-13 Ono, Daito, Osaka 574-0042, Japan.
AD  - Phonics Center, Osaka University, 2-1 Yamada-oka Suita, Osaka 565-0871, Japan.
FAU - Hirai, Koki
AU  - Hirai K
AD  - Department of Mechanical Engineering, Osaka University, 2-1 Yamada-oka, Suita, 
      Osaka 565-0871, Japan.
FAU - Inoue, Hiroaki
AU  - Inoue H
AD  - Aquatech Co., Ltd., 2-1-13 Ono, Daito, Osaka 574-0042, Japan.
FAU - Sakurai, Yoichi
AU  - Sakurai Y
AD  - Aquatech Co., Ltd., 2-1-13 Ono, Daito, Osaka 574-0042, Japan.
FAU - Yamada, Yoji
AU  - Yamada Y
AD  - Aquatech Co., Ltd., 2-1-13 Ono, Daito, Osaka 574-0042, Japan.
FAU - Taniguchi, Takashi
AU  - Taniguchi T
AD  - Aquatech Co., Ltd., 2-1-13 Ono, Daito, Osaka 574-0042, Japan.
FAU - Morishima, Keisuke
AU  - Morishima K
AD  - Department of Mechanical Engineering, Osaka University, 2-1 Yamada-oka, Suita, 
      Osaka 565-0871, Japan.
AD  - Global Center for Medical Engineering and Informatics, Osaka University, 2-1 
      Yamada-oka Suita, Osaka 565-0871, Japan.
LA  - eng
PT  - Journal Article
DEP - 20200423
PL  - Switzerland
TA  - Micromachines (Basel)
JT  - Micromachines
JID - 101640903
PMC - PMC7231339
OTO - NOTNLM
OT  - cell culture system
OT  - full-press ring pump
OT  - microfluidic system
OT  - mid-press ring pump
OT  - ring pump
COIS- The authors declare no conflict of interest.
EDAT- 2020/04/29 06:00
MHDA- 2020/04/29 06:01
PMCR- 2020/04/23
CRDT- 2020/04/29 06:00
PHST- 2020/02/05 00:00 [received]
PHST- 2020/04/14 00:00 [revised]
PHST- 2020/04/17 00:00 [accepted]
PHST- 2020/04/29 06:00 [entrez]
PHST- 2020/04/29 06:00 [pubmed]
PHST- 2020/04/29 06:01 [medline]
PHST- 2020/04/23 00:00 [pmc-release]
AID - mi11040447 [pii]
AID - micromachines-11-00447 [pii]
AID - 10.3390/mi11040447 [doi]
PST - epublish
SO  - Micromachines (Basel). 2020 Apr 23;11(4):447. doi: 10.3390/mi11040447.