PMID- 32340407
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 2076-2615 (Print)
IS  - 2076-2615 (Electronic)
IS  - 2076-2615 (Linking)
VI  - 10
IP  - 4
DP  - 2020 Apr 23
TI  - Expression of DAZL Gene in Selected Tissues and Association of Its Polymorphisms 
      with Testicular Size in Hu Sheep.
LID - 10.3390/ani10040740 [doi]
LID - 740
AB  - The deleted in azoospermia-like (DAZL) gene encoding an RNA binding protein is 
      pivotal in gametogenesis in lots of species and also acts as a pre-meiosis 
      marker. The current study was conducted to detect expression profiles and single 
      nucleotide polymorphisms (SNPs) of DAZL in sheep using qPCR, DNA-pooled 
      sequencing, improved multiplex ligase detection reaction (iMLDR((R))) and 
      restriction fragment length polymorphism (RFLP) methods. The results confirmed 
      that ovine DAZL showed the highest expression level at six-months of age across 
      five developmental stage. At six-month stage, DAZL expressed primarily in testis 
      across seven tissues analyzed. The abundance of DAZL in the large-testis group is 
      higher than that in the small-testis group although it is not significant. In 
      addition, six SNPs (SNP1-SNP6) were identified in DAZL. Of those, SNP1 (p < 0.05) 
      and SNP6 (p < 0.01) were significantly correlated with the variation coefficient 
      between left and right epididymis weight (VCTW). The current study implies DAZL 
      may play important roles in testicular development and its SNPs are associated 
      with testicular parameters, which supply important indicators for ram selection 
      at early stage.
FAU - Yuan, Zehu
AU  - Yuan Z
AD  - State Key Laboratory of Grassland Agro-Ecosystems, Key Laboratory of Grassland 
      Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, 
      Engineering Research Center of Grassland Industry, Ministry of Education, College 
      of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 
      730020, China.
FAU - Luo, Jing
AU  - Luo J
AD  - State Key Laboratory of Grassland Agro-Ecosystems, Key Laboratory of Grassland 
      Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, 
      Engineering Research Center of Grassland Industry, Ministry of Education, College 
      of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 
      730020, China.
FAU - Wang, Li
AU  - Wang L
AD  - State Key Laboratory of Grassland Agro-Ecosystems, Key Laboratory of Grassland 
      Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, 
      Engineering Research Center of Grassland Industry, Ministry of Education, College 
      of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 
      730020, China.
FAU - Li, Fadi
AU  - Li F
AD  - State Key Laboratory of Grassland Agro-Ecosystems, Key Laboratory of Grassland 
      Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, 
      Engineering Research Center of Grassland Industry, Ministry of Education, College 
      of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 
      730020, China.
AD  - Engineering Laboratory of Sheep Breeding and Reproduction Biotechnology in Gansu 
      Province, Minqin 733300, China.
FAU - Li, Wanhong
AU  - Li W
AD  - State Key Laboratory of Grassland Agro-Ecosystems, Key Laboratory of Grassland 
      Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, 
      Engineering Research Center of Grassland Industry, Ministry of Education, College 
      of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 
      730020, China.
FAU - Yue, Xiangpeng
AU  - Yue X
AD  - State Key Laboratory of Grassland Agro-Ecosystems, Key Laboratory of Grassland 
      Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, 
      Engineering Research Center of Grassland Industry, Ministry of Education, College 
      of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 
      730020, China.
LA  - eng
GR  - CARS-38/National Joint R&D Programme for Animal Breeding and China Agriculture 
      Research System/
PT  - Journal Article
DEP - 20200423
PL  - Switzerland
TA  - Animals (Basel)
JT  - Animals : an open access journal from MDPI
JID - 101635614
PMC - PMC7222755
OTO - NOTNLM
OT  - DAZL
OT  - Hu sheep
OT  - expression
OT  - polymorphism
OT  - testis
COIS- The authors declare no conflict of interest.
EDAT- 2020/04/29 06:00
MHDA- 2020/04/29 06:01
PMCR- 2020/04/23
CRDT- 2020/04/29 06:00
PHST- 2020/03/09 00:00 [received]
PHST- 2020/04/17 00:00 [revised]
PHST- 2020/04/20 00:00 [accepted]
PHST- 2020/04/29 06:00 [entrez]
PHST- 2020/04/29 06:00 [pubmed]
PHST- 2020/04/29 06:01 [medline]
PHST- 2020/04/23 00:00 [pmc-release]
AID - ani10040740 [pii]
AID - animals-10-00740 [pii]
AID - 10.3390/ani10040740 [doi]
PST - epublish
SO  - Animals (Basel). 2020 Apr 23;10(4):740. doi: 10.3390/ani10040740.