PMID- 32341026
OWN - NLM
STAT- MEDLINE
DCOM- 20201216
LR  - 20201216
IS  - 1477-9129 (Electronic)
IS  - 0950-1991 (Print)
IS  - 0950-1991 (Linking)
VI  - 147
IP  - 8
DP  - 2020 Apr 27
TI  - Smad4-dependent morphogenic signals control the maturation and axonal targeting 
      of basal vomeronasal sensory neurons to the accessory olfactory bulb.
LID - 10.1242/dev.184036 [doi]
LID - dev184036
AB  - The vomeronasal organ (VNO) contains two main types of vomeronasal sensory 
      neurons (VSNs) that express distinct vomeronasal receptor (VR) genes and localize 
      to specific regions of the neuroepithelium. Morphogenic signals are crucial in 
      defining neuronal identity and network formation; however, if and what signals 
      control maturation and homeostasis of VSNs is largely unexplored. Here, we found 
      transforming growth factor beta (TGFbeta) and bone morphogenetic protein (BMP) signal 
      transduction in postnatal mice, with BMP signaling being restricted to basal VSNs 
      and at the marginal zones of the VNO: the site of neurogenesis. Using different 
      Smad4 conditional knockout mouse models, we disrupted canonical TGFbeta/BMP 
      signaling in either maturing basal VSNs (bVSNs) or all mature VSNs. Smad4 loss of 
      function in immature bVSNs compromises dendritic knob formation, pheromone 
      induced activation, correct glomeruli formation in the accessory olfactory bulb 
      (AOB) and survival. However, Smad4 loss of function in all mature VSNs only 
      compromises correct glomeruli formation in the posterior AOB. Our results 
      indicate that Smad4-mediated signaling drives the functional maturation and 
      connectivity of basal VSNs.
CI  - (c) 2020. Published by The Company of Biologists Ltd.
FAU - Naik, Ankana S
AU  - Naik AS
AD  - Department of Biological Sciences; The RNA Institute; University at Albany, State 
      University of New York, Albany, NY 12222, USA.
FAU - Lin, Jennifer M
AU  - Lin JM
AUID- ORCID: 0000-0002-9197-0816
AD  - Department of Biological Sciences; The RNA Institute; University at Albany, State 
      University of New York, Albany, NY 12222, USA.
FAU - Taroc, Ed Zandro M
AU  - Taroc EZM
AUID- ORCID: 0000-0002-0873-4901
AD  - Department of Biological Sciences; The RNA Institute; University at Albany, State 
      University of New York, Albany, NY 12222, USA.
FAU - Katreddi, Raghu R
AU  - Katreddi RR
AD  - Department of Biological Sciences; The RNA Institute; University at Albany, State 
      University of New York, Albany, NY 12222, USA.
FAU - Frias, Jesus A
AU  - Frias JA
AD  - Department of Biological Sciences; The RNA Institute; University at Albany, State 
      University of New York, Albany, NY 12222, USA.
FAU - Lemus, Alex A
AU  - Lemus AA
AD  - Department of Biological Sciences; The RNA Institute; University at Albany, State 
      University of New York, Albany, NY 12222, USA.
FAU - Sammons, Morgan A
AU  - Sammons MA
AD  - Department of Biological Sciences; The RNA Institute; University at Albany, State 
      University of New York, Albany, NY 12222, USA.
FAU - Forni, Paolo E
AU  - Forni PE
AUID- ORCID: 0000-0001-6547-3464
AD  - Department of Biological Sciences; The RNA Institute; University at Albany, State 
      University of New York, Albany, NY 12222, USA pforni@albany.edu.
LA  - eng
GR  - R01 DC017149/DC/NIDCD NIH HHS/United States
GR  - R01 HD097331/HD/NICHD NIH HHS/United States
GR  - R15 GM128049/GM/NIGMS NIH HHS/United States
GR  - R15 HD096411/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20200427
PL  - England
TA  - Development
JT  - Development (Cambridge, England)
JID - 8701744
RN  - 0 (Bone Morphogenetic Proteins)
RN  - 0 (GAP-43 Protein)
RN  - 0 (Smad4 Protein)
RN  - 0 (Transforming Growth Factor beta)
RN  - EC 2.7.7.- (Cre recombinase)
RN  - EC 2.7.7.- (Integrases)
SB  - IM
MH  - Animals
MH  - Axons/*metabolism
MH  - Bone Morphogenetic Proteins/metabolism
MH  - Dendrites/metabolism
MH  - GAP-43 Protein/metabolism
MH  - Gene Deletion
MH  - Integrases/metabolism
MH  - Mice, Knockout
MH  - *Morphogenesis
MH  - Odorants
MH  - Olfactory Bulb/*metabolism
MH  - Presynaptic Terminals/metabolism
MH  - Sensory Receptor Cells/*metabolism
MH  - Signal Transduction
MH  - Smad4 Protein/*metabolism
MH  - Transcriptome/genetics
MH  - Transforming Growth Factor beta/metabolism
MH  - Vomeronasal Organ/*metabolism
PMC - PMC7197725
OTO - NOTNLM
OT  - Axonal targeting
OT  - BMP
OT  - Glomeruli
OT  - Olfactory
OT  - Smad4
OT  - Vomeronasal sensory neurons
COIS- Competing interestsThe authors declare no competing or financial interests.
EDAT- 2020/04/29 06:00
MHDA- 2020/12/17 06:00
PMCR- 2020/04/27
CRDT- 2020/04/29 06:00
PHST- 2019/08/20 00:00 [received]
PHST- 2020/03/10 00:00 [accepted]
PHST- 2020/04/29 06:00 [entrez]
PHST- 2020/04/29 06:00 [pubmed]
PHST- 2020/12/17 06:00 [medline]
PHST- 2020/04/27 00:00 [pmc-release]
AID - 147/8/dev184036 [pii]
AID - DEV184036 [pii]
AID - 10.1242/dev.184036 [doi]
PST - epublish
SO  - Development. 2020 Apr 27;147(8):dev184036. doi: 10.1242/dev.184036.