PMID- 32341347 OWN - NLM STAT- MEDLINE DCOM- 20210331 LR - 20210427 IS - 2041-4889 (Electronic) VI - 11 IP - 4 DP - 2020 Apr 27 TI - Exosomes derived from mesenchymal stem cells repair a Parkinson's disease model by inducing autophagy. PG - 288 LID - 10.1038/s41419-020-2473-5 [doi] LID - 288 AB - Parkinson's disease (PD) is a progressively debilitating neurodegenerative condition that leads to motor and cognitive dysfunction. At present, clinical treatment can only improve symptoms, but cannot effectively protect dopaminergic neurons. Several reports have demonstrated that human umbilical cord mesenchymal stem cells (hucMSCs) afford neuroprotection, while their application is limited because of their uncontrollable differentiation and other reasons. Stem cells communicate with cells through secreted exosomes (Exos), the present study aimed to explore whether Exos secreted by hucMSCs could function instead of hucMSCs. hucMSCs were successfully isolated and characterized, and shown to contribute to 6-hydroxydopamine (6-OHDA)-stimulated SH-SY5Y cell proliferation; hucMSC-derived Exos were also involved in this process. The Exos were purified and identified, and then labeled with PKH 26, it was found that the Exos could be efficiently taken up by SH-SY5Y cells after 12 h of incubation. Pretreatment with Exos promoted 6-OHDA-stimulated SH-SY5Y cells to proliferate and inhibited apoptosis by inducing autophagy. Furthermore, Exos reached the substantia nigra through the blood-brain barrier (BBB) in vivo, relieved apomorphine-induced asymmetric rotation, reduced substantia nigra dopaminergic neuron loss and apoptosis, and upregulated the level of dopamine in the striatum. These results demonstrate that hucMSCs-Exos have a treatment capability for PD and can traverse the BBB, indicating their potential for the effective treatment of PD. FAU - Chen, Hong-Xu AU - Chen HX AD - Department of Neurology, the First Hospital of Hebei Medical University, No. 89 Donggang Road, Yuhua District, Shi Jiazhuang, 050031, Hebei, China. AD - Hebei Provincial Engineering Laboratory of Plant Bioreactor Preparation Technology, No. 326 Xinshi south Road, Qiaoxi District, Shi Jiazhuang, 050090, Hebei, China. FAU - Liang, Fu-Chao AU - Liang FC AD - Hebei Provincial Engineering Laboratory of Plant Bioreactor Preparation Technology, No. 326 Xinshi south Road, Qiaoxi District, Shi Jiazhuang, 050090, Hebei, China. AD - Research Center, Hebei University of Chinese Medicine, No. 326 Xinshi south Road, Qiaoxi District, Shi Jiazhuang, 050090, Hebei, China. FAU - Gu, Ping AU - Gu P AD - Department of Neurology, the First Hospital of Hebei Medical University, No. 89 Donggang Road, Yuhua District, Shi Jiazhuang, 050031, Hebei, China. FAU - Xu, Bian-Ling AU - Xu BL AD - Hebei Provincial Engineering Laboratory of Plant Bioreactor Preparation Technology, No. 326 Xinshi south Road, Qiaoxi District, Shi Jiazhuang, 050090, Hebei, China. AD - Department of Gynecology, the Second Hospital of Hebei Medical University, No. 215, HePing West Road, Shi Jiazhuang, 050000, Hebei, China. FAU - Xu, Hong-Jun AU - Xu HJ AD - Hebei Provincial Engineering Laboratory of Plant Bioreactor Preparation Technology, No. 326 Xinshi south Road, Qiaoxi District, Shi Jiazhuang, 050090, Hebei, China. AD - Research Center, Hebei University of Chinese Medicine, No. 326 Xinshi south Road, Qiaoxi District, Shi Jiazhuang, 050090, Hebei, China. FAU - Wang, Wen-Ting AU - Wang WT AD - Department of Neurology, the First Hospital of Hebei Medical University, No. 89 Donggang Road, Yuhua District, Shi Jiazhuang, 050031, Hebei, China. FAU - Hou, Jia-Yang AU - Hou JY AD - Hebei Provincial Engineering Laboratory of Plant Bioreactor Preparation Technology, No. 326 Xinshi south Road, Qiaoxi District, Shi Jiazhuang, 050090, Hebei, China. AD - Research Center, Hebei University of Chinese Medicine, No. 326 Xinshi south Road, Qiaoxi District, Shi Jiazhuang, 050090, Hebei, China. FAU - Xie, Dong-Xiao AU - Xie DX AD - Department of orthopaedic, Third hospital of Hebei Medical University, Shi Jiazhuang, 050000, Hebei, China. FAU - Chai, Xi-Qing AU - Chai XQ AD - Department of Neurology, the First Hospital of Hebei Medical University, No. 89 Donggang Road, Yuhua District, Shi Jiazhuang, 050031, Hebei, China. chenhbykdx@126.com. FAU - An, Sheng-Jun AU - An SJ AD - Hebei Provincial Engineering Laboratory of Plant Bioreactor Preparation Technology, No. 326 Xinshi south Road, Qiaoxi District, Shi Jiazhuang, 050090, Hebei, China. sjsjan@126.com. AD - Research Center, Hebei University of Chinese Medicine, No. 326 Xinshi south Road, Qiaoxi District, Shi Jiazhuang, 050090, Hebei, China. sjsjan@126.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20200427 PL - England TA - Cell Death Dis JT - Cell death & disease JID - 101524092 SB - IM MH - Animals MH - Autophagy MH - Blood-Brain Barrier/*physiopathology MH - Cell Differentiation MH - Exosomes/*metabolism MH - Humans MH - Mesenchymal Stem Cells/*metabolism MH - Mice MH - Parkinson Disease/*genetics/physiopathology PMC - PMC7184757 COIS- The authors declare that they have no conflict of interest. EDAT- 2020/04/29 06:00 MHDA- 2021/04/01 06:00 PMCR- 2020/04/27 CRDT- 2020/04/29 06:00 PHST- 2019/12/08 00:00 [received] PHST- 2020/04/08 00:00 [accepted] PHST- 2020/04/07 00:00 [revised] PHST- 2020/04/29 06:00 [entrez] PHST- 2020/04/29 06:00 [pubmed] PHST- 2021/04/01 06:00 [medline] PHST- 2020/04/27 00:00 [pmc-release] AID - 10.1038/s41419-020-2473-5 [pii] AID - 2473 [pii] AID - 10.1038/s41419-020-2473-5 [doi] PST - epublish SO - Cell Death Dis. 2020 Apr 27;11(4):288. doi: 10.1038/s41419-020-2473-5.