PMID- 32342310
OWN - NLM
STAT- MEDLINE
DCOM- 20210126
LR  - 20210126
IS  - 1608-3091 (Electronic)
IS  - 1607-6729 (Linking)
VI  - 490
IP  - 1
DP  - 2020 Jan
TI  - Characteristic of Dopamine-Producing System and Dopamine Receptors in the 
      Suprachiasmatic Nucleus in Rats in Ontogenesis.
PG  - 34-37
LID - 10.1134/S1607672920010123 [doi]
AB  - One of the features of the developing suprachiasmatic nucleus (SCN), the 
      "biological clock" of the body, is the early expression of dopamine (DA) 
      receptors in the absence of dopaminergic neurons as a source of DA. Only recently 
      we showed that DA in SCN is synthesized together by nerve fibers containing only 
      tyrosine hydroxylase (TH) and neurons containing only aromatic L-amino acid 
      decarboxylase (AADC). This study was aimed to assess specific characteristics of 
      the phenotype of TH-fibers in ontogenesis. For this purpose, PCR and 
      immunohistochemical analysis of the expression of genes and proteins such as TH, 
      AADC, vesicular monoamine transporter (VMAT), and receptors for DA (D1, D2) was 
      performed. We have detected numerous TH-immunoreactive fibers in SCN of young and 
      adult rats. VMAT was observed in some of them, which suggests vesicular storage 
      of L-DOPA. Considering the key role of TH-fibers in cooperative synthesis of DA, 
      we assumed the presence of their dopamine regulation. Using double 
      immunolabeling, we showed that D1 and D2 are present in TH-fibers in adult rats, 
      and only D1 in young rats. According to PCR, D1 and D2 are also expressed in 
      neurons of SCN in adult rats and only D1 in young rats. Thus, it was shown for 
      the first time that VMAT and D1 are coexpressed in TH-fibers synthesizing L-DOPA 
      in SCN in young and adult rats, and also D2 receptors in adult rats, which 
      suggests vesicular storage and dopamine regulation of L-DOPA secretion, 
      respectively.
FAU - Pronina, T S
AU  - Pronina TS
AD  - Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia. 
      tatiana.pronina@mail.ru.
FAU - Kolacheva, A A
AU  - Kolacheva AA
AD  - Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia.
FAU - Dil'muhametova, L K
AU  - Dil'muhametova LK
AD  - Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia.
FAU - Nikishina, Yu O
AU  - Nikishina YO
AD  - Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia.
FAU - Suhinich, K K
AU  - Suhinich KK
AD  - Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia.
FAU - Ugrumov, M V
AU  - Ugrumov MV
AD  - Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia.
LA  - eng
PT  - Journal Article
DEP - 20200427
PL  - United States
TA  - Dokl Biochem Biophys
JT  - Doklady. Biochemistry and biophysics
JID - 101126895
RN  - 0 (Receptors, Dopamine)
RN  - 0 (Receptors, Dopamine D1)
RN  - 0 (Slc18a2 protein, rat)
RN  - 0 (Vesicular Monoamine Transport Proteins)
RN  - 46627O600J (Levodopa)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
RN  - EC 4.1.1.28 (Aromatic-L-Amino-Acid Decarboxylases)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Animals
MH  - Aromatic-L-Amino-Acid Decarboxylases/*metabolism
MH  - Dopamine/*metabolism
MH  - Dopaminergic Neurons/metabolism
MH  - Levodopa/metabolism
MH  - Male
MH  - Nerve Fibers/metabolism
MH  - Neurons/*metabolism
MH  - Phenotype
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, Dopamine/*metabolism
MH  - Receptors, Dopamine D1/metabolism
MH  - Suprachiasmatic Nucleus/*metabolism
MH  - Tyrosine 3-Monooxygenase/*metabolism
MH  - Vesicular Monoamine Transport Proteins/metabolism
OTO - NOTNLM
OT  - PCR
OT  - immunohistochemistry
OT  - laser microdissection
OT  - suprachiasmatic nucleus
OT  - tyrosine hydroxylase
EDAT- 2020/04/29 06:00
MHDA- 2021/01/27 06:00
CRDT- 2020/04/29 06:00
PHST- 2019/10/30 00:00 [received]
PHST- 2019/10/30 00:00 [accepted]
PHST- 2019/10/30 00:00 [revised]
PHST- 2020/04/29 06:00 [entrez]
PHST- 2020/04/29 06:00 [pubmed]
PHST- 2021/01/27 06:00 [medline]
AID - 10.1134/S1607672920010123 [pii]
AID - 10.1134/S1607672920010123 [doi]
PST - ppublish
SO  - Dokl Biochem Biophys. 2020 Jan;490(1):34-37. doi: 10.1134/S1607672920010123. Epub 
      2020 Apr 27.