PMID- 32345121
OWN - NLM
STAT- MEDLINE
DCOM- 20210607
LR  - 20210607
IS  - 1532-2335 (Electronic)
IS  - 1525-7770 (Linking)
VI  - 39
IP  - 10-12
DP  - 2020
TI  - Identification of a novel thymidylate kinase activity.
PG  - 1359-1368
LID - 10.1080/15257770.2020.1755043 [doi]
AB  - Thymidylate kinase (TMPK, EC2.7.4.9) is the enzyme that converts deoxythymidine 
      monophosphate (dTMP) to deoxythymidine diphosphate (dTDP) in the synthesis of 
      dTTP, an essential building block of DNA. To date, there is only one gene (TYMK) 
      known to encode TMPK in mammalian cells. In this study, we investigated the 
      distribution of TMPK activity and protein in subcellular fractions by using 
      activity measurements and by using a specific antibody against TYMK-encoded TMPK 
      (canonical TMPK). TMPK activity was detected in all subcellular fractions, of 
      which the mitochondrial outer membrane contained the highest activity. High 
      levels of canonical TMPK protein were detected in the cytosolic fraction, whereas 
      low levels were found in the nuclear and mitochondrial matrix fractions. 
      Strikingly, despite the detection of high TMPK activity in the mitochondrial 
      outer membrane, canonical TMPK protein was not detected in this fraction. These 
      results suggest that the TMPK activity detected in the outer membrane fraction 
      may originate from a novel dTMP kinase, distinct from the canonical TYMK.
FAU - Frisk, Junmei Hu
AU  - Frisk JH
AD  - Department of Anatomy, Physiology and Biochemistry, Swedish University of 
      Agricultural Sciences, Uppsala, Sweden.
FAU - Eriksson, Staffan
AU  - Eriksson S
AD  - Department of Anatomy, Physiology and Biochemistry, Swedish University of 
      Agricultural Sciences, Uppsala, Sweden.
FAU - Pejler, Gunnar
AU  - Pejler G
AD  - Department of Anatomy, Physiology and Biochemistry, Swedish University of 
      Agricultural Sciences, Uppsala, Sweden.
AD  - Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, 
      Sweden.
FAU - Wang, Liya
AU  - Wang L
AD  - Department of Anatomy, Physiology and Biochemistry, Swedish University of 
      Agricultural Sciences, Uppsala, Sweden.
LA  - eng
PT  - Journal Article
DEP - 20200428
PL  - United States
TA  - Nucleosides Nucleotides Nucleic Acids
JT  - Nucleosides, nucleotides & nucleic acids
JID - 100892832
RN  - EC 2.7.4.4 (Nucleoside-Phosphate Kinase)
RN  - EC 2.7.4.9 (dTMP kinase)
SB  - IM
MH  - Cell Line
MH  - Humans
MH  - Intracellular Space/metabolism
MH  - Kinetics
MH  - Nucleoside-Phosphate Kinase/*metabolism
MH  - Protein Transport
OTO - NOTNLM
OT  - dTTP synthesis
OT  - mitochondrial outer membrane
OT  - subcellular fractions
OT  - thymidylate kinase (TMPK)
EDAT- 2020/04/30 06:00
MHDA- 2021/06/08 06:00
CRDT- 2020/04/30 06:00
PHST- 2020/04/30 06:00 [pubmed]
PHST- 2021/06/08 06:00 [medline]
PHST- 2020/04/30 06:00 [entrez]
AID - 10.1080/15257770.2020.1755043 [doi]
PST - ppublish
SO  - Nucleosides Nucleotides Nucleic Acids. 2020;39(10-12):1359-1368. doi: 
      10.1080/15257770.2020.1755043. Epub 2020 Apr 28.