PMID- 32346066
OWN - NLM
STAT- MEDLINE
DCOM- 20201125
LR  - 20210428
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 10
IP  - 1
DP  - 2020 Apr 28
TI  - Immunoinflammatory response and bioactive potential of GuttaFlow bioseal and MTA 
      Fillapex in the rat subcutaneous tissue.
PG  - 7173
LID - 10.1038/s41598-020-64041-0 [doi]
LID - 7173
AB  - To evaluate the effect of GuttaFlow bioseal (GFB) and MTA Fillapex (MTAF) in 
      comparison with Endofill (EF) in the subcutaneous tissue. Polyethylene tubes with 
      GFB, MTAF, EF or empty tubes (control group; CG) were implanted into subcutaneous 
      of rats. After 7, 15, 30 and 60 days, the capsule thickness, inflammatory 
      reaction, interleukin-6 (IL-6), vascular endothelial growth factor (VEGF), 
      caspase-3, TUNEL-positive cells, von Kossa and ultrastructural features were 
      evaluated. The data were statistically analyzed (p </= 0.05). At all periods, the 
      number of IL-6- and VEGF-immunolabelled cells, and capsule thickness were lower 
      in GFB than MTAF, which was lower than EF (p < 0.0001). At 60 days, the number of 
      inflammatory cells was similar in GFB and MTAF (p = 0.58). Significant 
      differences in the number of TUNEL- and caspase-3-positive cells were not 
      observed among GFB, MTAF and CG whereas the highest values were found in EF 
      specimens. The EF specimens exhibited several cells with condensed chromatin, 
      typical of apoptosis. von Kossa-positive and birefringent structures were only 
      observed in GFB and MTAF, suggesting the presence of calcite crystals. Taken 
      together, these results show that cellular and structural damage induced by GFB 
      and MTAF sealers were recovery over time. Moreover, these sealers express 
      bioactive potential in subcutaneous tissue.
FAU - Delfino, Mateus Machado
AU  - Delfino MM
AD  - Department of Restorative Dentistry, Dental School - Sao Paulo State University 
      (UNESP), Araraquara, SP, Brazil.
FAU - Guerreiro-Tanomaru, Juliane Maria
AU  - Guerreiro-Tanomaru JM
AD  - Department of Restorative Dentistry, Dental School - Sao Paulo State University 
      (UNESP), Araraquara, SP, Brazil.
FAU - Tanomaru-Filho, Mario
AU  - Tanomaru-Filho M
AD  - Department of Restorative Dentistry, Dental School - Sao Paulo State University 
      (UNESP), Araraquara, SP, Brazil.
FAU - Sasso-Cerri, Estela
AU  - Sasso-Cerri E
AD  - Department of Morphology, Genetics, Orthodontics and Pediatric Dentistry - 
      Laboratory of Histology and Embryology, Dental School - Sao Paulo State 
      University (UNESP), Araraquara, SP, Brazil.
FAU - Cerri, Paulo Sergio
AU  - Cerri PS
AD  - Department of Morphology, Genetics, Orthodontics and Pediatric Dentistry - 
      Laboratory of Histology and Embryology, Dental School - Sao Paulo State 
      University (UNESP), Araraquara, SP, Brazil. paulo.cerri@unesp.br.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200428
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Bioseal)
RN  - 0 (Dimethylpolysiloxanes)
RN  - 0 (Drug Combinations)
RN  - 0 (GuttaFlow)
RN  - 0 (Il6 protein, rat)
RN  - 0 (Interleukin-6)
RN  - 0 (Root Canal Filling Materials)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (vascular endothelial growth factor A, rat)
RN  - 9000-32-2 (Gutta-Percha)
RN  - EC 3.4.22.- (Casp3 protein, rat)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects/immunology
MH  - Caspase 3/immunology
MH  - Dimethylpolysiloxanes/*pharmacology
MH  - Drug Combinations
MH  - Gutta-Percha/*pharmacology
MH  - Inflammation/immunology/pathology
MH  - Interleukin-6/immunology
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Root Canal Filling Materials/*pharmacology
MH  - Subcutaneous Tissue/*immunology/pathology
MH  - Vascular Endothelial Growth Factor A/immunology
PMC - PMC7188821
COIS- The authors declare no competing interests.
EDAT- 2020/04/30 06:00
MHDA- 2020/11/26 06:00
PMCR- 2020/04/28
CRDT- 2020/04/30 06:00
PHST- 2019/07/04 00:00 [received]
PHST- 2019/12/06 00:00 [accepted]
PHST- 2020/04/30 06:00 [entrez]
PHST- 2020/04/30 06:00 [pubmed]
PHST- 2020/11/26 06:00 [medline]
PHST- 2020/04/28 00:00 [pmc-release]
AID - 10.1038/s41598-020-64041-0 [pii]
AID - 64041 [pii]
AID - 10.1038/s41598-020-64041-0 [doi]
PST - epublish
SO  - Sci Rep. 2020 Apr 28;10(1):7173. doi: 10.1038/s41598-020-64041-0.