PMID- 32348913
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20210903
IS  - 2213-2201 (Electronic)
IS  - 2213-2198 (Print)
VI  - 8
IP  - 8
DP  - 2020 Sep
TI  - Microarray-Based Detection of Allergen-Reactive IgE in Patients with 
      Mastocytosis.
PG  - 2761-2768.e16
LID - S2213-2198(20)30382-2 [pii]
LID - 10.1016/j.jaip.2020.04.030 [doi]
AB  - BACKGROUND: Because of a high risk to develop fatal anaphylaxis, early detection 
      of immunoglobulin E (IgE)-dependent allergy is of particular importance in 
      patients with mastocytosis. OBJECTIVE: We examined whether microarray-based 
      screening for allergen-reactive IgE (allergen-chip) is a sensitive and robust 
      approach to detect specific IgE in patients with mastocytosis. METHODS: Sera for 
      42 patients were analyzed, including 4 with cutaneous mastocytosis, 2 with 
      mastocytosis in the skin, and 36 with systemic mastocytosis. In addition, sera 
      from an age- and sex-matched control cohort (n = 42) were analyzed. RESULTS: In 
      15 of 42 patients with mastocytosis (35.7%), specific IgE was detected by 
      allergen-chip profiling. Ves v 5 and Bet v 1 were the most frequently detected 
      allergens (Ves v 5: 16.7% of patients; Bet v 1: 11.9% of patients). Allergen 
      reactivity was confirmed by demonstrating upregulation of CD203c on blood 
      basophils upon exposure to the respective allergen(s) in these patients. Specific 
      IgE was identified by chip studies in 11 of 26 patients with mastocytosis with 
      mediator-related symptoms (42.3%) and in 4 of 14 patients with mastocytosis 
      without symptoms (28.6%). In the cohort with known allergy, 9 of 9 patients 
      (100%) had a positive allergen-chip result. In patients with mastocytosis without 
      a known allergy (n = 31), the chip identified 6 positive cases (19.5%). The 
      prevalence of chip-positive patients was slightly lower in the mastocytosis group 
      (35.7%) compared with age- and sex-matched controls (40.5%). CONCLUSIONS: 
      Although specific IgE may not be detectable in all sensitized patients with 
      mastocytosis, allergy chip-profiling is a reliable screening approach for the 
      identification of patients with mastocytosis suffering from IgE-dependent 
      allergies.
CI  - Copyright (c) 2020 American Academy of Allergy, Asthma & Immunology. Published by 
      Elsevier Inc. All rights reserved.
FAU - Smiljkovic, Dubravka
AU  - Smiljkovic D
AD  - Department of Internal Medicine I, Division of Hematology & Hemostaseology, 
      Medical University of Vienna, Vienna, Austria.
FAU - Kiss, Renata
AU  - Kiss R
AD  - Department of Pathophysiology and Allergy Research, Division of Immunopathology, 
      Center for Pathophysiology, Immunology and Infectiology, Medical University of 
      Vienna, Vienna, Austria.
FAU - Lupinek, Christian
AU  - Lupinek C
AD  - Department of Pathophysiology and Allergy Research, Division of Immunopathology, 
      Center for Pathophysiology, Immunology and Infectiology, Medical University of 
      Vienna, Vienna, Austria.
FAU - Hoermann, Gregor
AU  - Hoermann G
AD  - Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria; 
      Ludwig Boltzmann Institute for Hematology and Oncology, Medical University of 
      Vienna, Vienna, Austria; Central Institute of Medical and Chemical Laboratory 
      Diagnostics, University Hospital Innsbruck, Innsbruck, Austria.
FAU - Greiner, Georg
AU  - Greiner G
AD  - Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria; 
      Ludwig Boltzmann Institute for Hematology and Oncology, Medical University of 
      Vienna, Vienna, Austria.
FAU - Witzeneder, Nadine
AU  - Witzeneder N
AD  - Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria.
FAU - Krajnik, Gerhard
AU  - Krajnik G
AD  - Department of Internal Medicine I, University Hospital St. Poelten, St. Poelten, 
      Austria.
FAU - Trautinger, Franz
AU  - Trautinger F
AD  - Department of Dermatology, University Hospital St. Poelten, St. Poelten, Austria.
FAU - Vrtala, Susanne
AU  - Vrtala S
AD  - Department of Pathophysiology and Allergy Research, Division of Immunopathology, 
      Center for Pathophysiology, Immunology and Infectiology, Medical University of 
      Vienna, Vienna, Austria.
FAU - Mittermann, Irene
AU  - Mittermann I
AD  - Department of Pathophysiology and Allergy Research, Division of Immunopathology, 
      Center for Pathophysiology, Immunology and Infectiology, Medical University of 
      Vienna, Vienna, Austria.
FAU - Kundi, Michael
AU  - Kundi M
AD  - Institute of Environmental Health of the Medical University of Vienna, Vienna, 
      Austria.
FAU - Jilma, Bernd
AU  - Jilma B
AD  - Department of Clinical Pharmacology, Medical University of Vienna, Vienna, 
      Austria.
FAU - Valenta, Rudolf
AU  - Valenta R
AD  - Department of Pathophysiology and Allergy Research, Division of Immunopathology, 
      Center for Pathophysiology, Immunology and Infectiology, Medical University of 
      Vienna, Vienna, Austria; NRC Institute of Immunology FMBA of Russia, Moscow, 
      Russia; Laboratory for Immunopathology, Department of Clinical Immunology and 
      Allergy, Sechenov First Moscow State Medical University, Moscow, Russia; Karl 
      Landsteiner University of Health Sciences, Krems, Austria.
FAU - Sperr, Wolfgang R
AU  - Sperr WR
AD  - Department of Internal Medicine I, Division of Hematology & Hemostaseology, 
      Medical University of Vienna, Vienna, Austria; Ludwig Boltzmann Institute for 
      Hematology and Oncology, Medical University of Vienna, Vienna, Austria. 
      Electronic address: wolfgang.r.sperr@meduniwien.ac.at.
FAU - Valent, Peter
AU  - Valent P
AD  - Department of Internal Medicine I, Division of Hematology & Hemostaseology, 
      Medical University of Vienna, Vienna, Austria; Ludwig Boltzmann Institute for 
      Hematology and Oncology, Medical University of Vienna, Vienna, Austria. 
      Electronic address: peter.valent@meduniwien.ac.at.
LA  - eng
GR  - F 4605/FWF_/Austrian Science Fund FWF/Austria
GR  - F 4704/FWF_/Austrian Science Fund FWF/Austria
GR  - P 26728/FWF_/Austrian Science Fund FWF/Austria
GR  - P 32470/FWF_/Austrian Science Fund FWF/Austria
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200426
PL  - United States
TA  - J Allergy Clin Immunol Pract
JT  - The journal of allergy and clinical immunology. In practice
JID - 101597220
RN  - 0 (Allergens)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens
MH  - *Anaphylaxis
MH  - Basophils
MH  - Humans
MH  - Immunoglobulin E
MH  - *Mastocytosis
PMC - PMC7116130
MID - EMS95148
OTO - NOTNLM
OT  - Allergen-chip
OT  - Allergy
OT  - IgE
OT  - Mastocytosis
COIS- Conflicts of interest: C. Lupinek received honoraria from Thermo Fisher for 
      lectures given at symposia for continuing medical education. R. Valenta received 
      research grants from Biomay AG, Vienna, Austria, and Viravaxx, Vienna, Austria, 
      and serves as a consultant for these companies. W. R. Sperr received a research 
      grant from Thermo Fisher and honoraria from Novartis, Pfizer, Deciphera, Jazz, 
      Teva, and Celgene. P. Valent received research grants from Celgene, Incyte, 
      Pfizer, Deciphera, and Blueprint and honoraria from Novartis, Pfizer, Deciphera, 
      Accord, and Celgene. The rest of the authors declare that they have no relevant 
      conflicts of interest.
EDAT- 2020/04/30 06:00
MHDA- 2021/05/15 06:00
PMCR- 2021/09/01
CRDT- 2020/04/30 06:00
PHST- 2019/09/03 00:00 [received]
PHST- 2020/04/03 00:00 [revised]
PHST- 2020/04/11 00:00 [accepted]
PHST- 2020/04/30 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
PHST- 2020/04/30 06:00 [entrez]
PHST- 2021/09/01 00:00 [pmc-release]
AID - S2213-2198(20)30382-2 [pii]
AID - 10.1016/j.jaip.2020.04.030 [doi]
PST - ppublish
SO  - J Allergy Clin Immunol Pract. 2020 Sep;8(8):2761-2768.e16. doi: 
      10.1016/j.jaip.2020.04.030. Epub 2020 Apr 26.