PMID- 32351607
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240328
IS  - 1741-427X (Print)
IS  - 1741-4288 (Electronic)
IS  - 1741-427X (Linking)
VI  - 2020
DP  - 2020
TI  - RNA-Seq Analysis of the Liver Transcriptome Reveals the Networks Regulating 
      Treatment of Sitagliptin Phosphate plus Fuzhujiangtang Granule in the Zucker 
      Diabetic Fatty Rats.
PG  - 8463858
LID - 10.1155/2020/8463858 [doi]
LID - 8463858
AB  - Diabetes is one of the most serious chronic diseases. Numerous drugs including 
      oral agents and traditional Chinese medicines, such as sitagliptin phosphate (SP) 
      and Fuzhujiangtang granules (FJG), have been discovered to treat diabetes and 
      used in combination in clinical practice. However, the exact effect and 
      underlying mechanism of using combined medicine is not clear. In this study, we 
      compared the antidiabetic effect of SP, FJG, and SP plus FJG (SP-FJG) using forty 
      8-week-old Zucker diabetic fatty (ZDF) rats and 10 age-matched Zucker lean rats 
      as the normal control group. ZDF rats were treated with different therapies, 
      respectively, for 6 weeks. The study showed that the fast blood glucose, random 
      blood glucose (RBG), oral glucose tolerance test (OGTT), insulin tolerance test 
      (ITT), homeostasis model of assessment-insulin resistance index, triglyceride 
      (TC), superoxide dismutase, and malondialdehyde of each treatment group were 
      improved when compared with the diabetes mellitus (DM) control group. Using 
      SP-FJG in combination had better improvements in OGTT, fast serum insulin levels, 
      TNF-alpha, and IL-6 compared with using SP individually. Besides, the increased LDL 
      and TC caused by using SP was attenuated by using FJG in combination. Meanwhile, 
      compared with the DM group, 1781 differentially expressed genes (DEGs) (including 
      1248 mRNA, 211 ncRNA, 202 cirRNA, and 120 miRNA) were enriched in 58 pathways. 
      Through analysis of ceRNA networks, we found that rno-miR-326-3p, rno-miR-423-5p, 
      rno-miR-15b-5p, rno-let-7c-5p, and rno-let-7b-5p were related to pharmacodynamics 
      in different groups. By analyzing the protein-protein interaction (PPI) and 
      coexpression networks of the transcriptomes of different groups, it is inferred 
      that Lrrk2 and Irak3 may be pharmacodynamic genes for type 2 diabetes mellitus 
      (T2DM). Our research compared the treatment of SP, FJG, and SP-FJG and acquainted 
      the PPI network, coexpression network, mutations, and pharmacodynamics genes, 
      which reveals the new mechanisms of pathogenesis of T2DM.
CI  - Copyright (c) 2020 Xuan Guo et al.
FAU - Guo, Xuan
AU  - Guo X
AD  - Dongfang Hospital of Beijing University of Chinese Medicine, Beijing 100078, 
      China.
AD  - Key Laboratory of Health Cultivation of the Ministry of Education, Beijing 
      University of Chinese Medicine, Beijing 100029, China.
FAU - Sun, Wen
AU  - Sun W
AD  - Key Laboratory of Health Cultivation of the Ministry of Education, Beijing 
      University of Chinese Medicine, Beijing 100029, China.
AD  - Beijing Key Laboratory of Health Cultivation, Beijing University of Chinese 
      Medicine, Beijing 100029, China.
FAU - Xu, Guangyuan
AU  - Xu G
AUID- ORCID: 0000-0001-9068-6728
AD  - Department of Traditional Chinese Medicine, Fu Xing Hospital of Capital Medical 
      University, Beijing 100045, China.
FAU - Hou, Dan
AU  - Hou D
AD  - Key Laboratory of Health Cultivation of the Ministry of Education, Beijing 
      University of Chinese Medicine, Beijing 100029, China.
FAU - Zhang, Zhuo
AU  - Zhang Z
AD  - Key Laboratory of Health Cultivation of the Ministry of Education, Beijing 
      University of Chinese Medicine, Beijing 100029, China.
FAU - Wu, Lili
AU  - Wu L
AUID- ORCID: 0000-0001-8601-0637
AD  - Key Laboratory of Health Cultivation of the Ministry of Education, Beijing 
      University of Chinese Medicine, Beijing 100029, China.
AD  - Beijing Key Laboratory of Health Cultivation, Beijing University of Chinese 
      Medicine, Beijing 100029, China.
FAU - Liu, Tonghua
AU  - Liu T
AUID- ORCID: 0000-0001-7015-5348
AD  - Dongfang Hospital of Beijing University of Chinese Medicine, Beijing 100078, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20200413
PL  - United States
TA  - Evid Based Complement Alternat Med
JT  - Evidence-based complementary and alternative medicine : eCAM
JID - 101215021
PMC - PMC7174946
COIS- The authors declare that they have no conflicts of interest.
EDAT- 2020/05/01 06:00
MHDA- 2020/05/01 06:01
PMCR- 2020/04/13
CRDT- 2020/05/01 06:00
PHST- 2019/02/22 00:00 [received]
PHST- 2020/03/12 00:00 [accepted]
PHST- 2020/05/01 06:00 [entrez]
PHST- 2020/05/01 06:00 [pubmed]
PHST- 2020/05/01 06:01 [medline]
PHST- 2020/04/13 00:00 [pmc-release]
AID - 10.1155/2020/8463858 [doi]
PST - epublish
SO  - Evid Based Complement Alternat Med. 2020 Apr 13;2020:8463858. doi: 
      10.1155/2020/8463858. eCollection 2020.