PMID- 32351986
OWN - NLM
STAT- MEDLINE
DCOM- 20210211
LR  - 20240329
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2020
DP  - 2020
TI  - miR-30a-5p Inhibits Proliferation and Migration of Lung Squamous Cell Carcinoma 
      Cells by Targeting FOXD1.
PG  - 2547902
LID - 10.1155/2020/2547902 [doi]
LID - 2547902
AB  - OBJECTIVE: To investigate the mechanism of miR-30a-5p inhibiting proliferation 
      and migration of lung squamous cell carcinoma (LSCC) cells by targeting FOXD1. 
      METHODS: Bioinformatics was used to analyze differentially expressed genes in the 
      TCGA_LUSC database. qRT-PCR was used to detect the expression levels of 
      miR-30a-5p and FOXD1 in human normal lung epithelial cell line and human LSCC 
      cell lines. The protein expression of FOXD1 was detected by western blot. The 
      cell viability and colony formation abilities were examined by CCK-8 and colony 
      formation assays, respectively. Wound healing and Transwell assays were performed 
      to examine the migration and invasion abilities of cells. The targeted binding 
      sites of miR-30a-5p and FOXD1 were predicted by bioinformatics, and dual 
      luciferase assay was used to verify the targeted binding relationship between 
      miR-30a-5p and FOXD1. RESULT: miR-30a-5p was downregulated in LSCC tissues and 
      cells, while FOXD1 was highly expressed. Overexpression of miR-30a-5p or 
      silencing FOXD1 inhibited cell viability, colony formation ability, migration, 
      and invasion of LSCC cells. miR-30a-5p inhibited the proliferation and migration 
      of LSCC cells by downregulating the expression of FOXD1. CONCLUSION: miR-30a-5p 
      can downregulate the expression of FOXD1 and inhibit the proliferation and 
      migration of LSCC.
CI  - Copyright (c) 2020 Chunhua Chen et al.
FAU - Chen, Chunhua
AU  - Chen C
AUID- ORCID: 0000-0002-6568-4111
AD  - Department of Pulmonary and Critical Care Medicine, Sir Run Run Shaw Hospital, 
      School of Medicine, Zhejiang University, Hangzhou 310016, China.
FAU - Tang, Junhua
AU  - Tang J
AUID- ORCID: 0000-0002-7219-927X
AD  - Department of Respiratory Medicine, First People's Hospital of Fuyang, Hangzhou 
      311400, China.
FAU - Xu, Shan
AU  - Xu S
AUID- ORCID: 0000-0003-2586-6719
AD  - Department of Pulmonary and Critical Care Medicine, Sir Run Run Shaw Hospital, 
      School of Medicine, Zhejiang University, Hangzhou 310016, China.
FAU - Zhang, Wenxia
AU  - Zhang W
AUID- ORCID: 0000-0002-0952-5482
AD  - Department of Pulmonary and Critical Care Medicine, Sir Run Run Shaw Hospital, 
      School of Medicine, Zhejiang University, Hangzhou 310016, China.
FAU - Jiang, Hanliang
AU  - Jiang H
AUID- ORCID: 0000-0003-0902-882X
AD  - Department of Pulmonary and Critical Care Medicine, Sir Run Run Shaw Hospital, 
      School of Medicine, Zhejiang University, Hangzhou 310016, China.
LA  - eng
PT  - Journal Article
PT  - Retracted Publication
DEP - 20200413
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (FOXD1 protein, human)
RN  - 0 (Forkhead Transcription Factors)
RN  - 0 (MIRN30b microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (RNA, Neoplasm)
SB  - IM
RIN - Biomed Res Int. 2024 Mar 20;2024:9868249. PMID: 38550077
MH  - Carcinoma, Squamous Cell/genetics/*metabolism/pathology
MH  - Cell Line, Tumor
MH  - *Cell Movement
MH  - *Cell Proliferation
MH  - Forkhead Transcription Factors/*biosynthesis/genetics
MH  - Humans
MH  - Lung Neoplasms/genetics/*metabolism/pathology
MH  - MicroRNAs/genetics/*metabolism
MH  - Neoplasm Proteins/*biosynthesis/genetics
MH  - RNA, Neoplasm/genetics/*metabolism
PMC - PMC7174912
COIS- The authors declare that they have no potential conflicts of interest.
EDAT- 2020/05/01 06:00
MHDA- 2021/02/12 06:00
PMCR- 2020/04/13
CRDT- 2020/05/01 06:00
PHST- 2020/02/19 00:00 [received]
PHST- 2020/03/23 00:00 [accepted]
PHST- 2020/05/01 06:00 [entrez]
PHST- 2020/05/01 06:00 [pubmed]
PHST- 2021/02/12 06:00 [medline]
PHST- 2020/04/13 00:00 [pmc-release]
AID - 10.1155/2020/2547902 [doi]
PST - epublish
SO  - Biomed Res Int. 2020 Apr 13;2020:2547902. doi: 10.1155/2020/2547902. eCollection 
      2020.