PMID- 32353641
OWN - NLM
STAT- MEDLINE
DCOM- 20210414
LR  - 20210414
IS  - 1879-0887 (Electronic)
IS  - 0167-8140 (Linking)
VI  - 148
DP  - 2020 Jul
TI  - Long-term defects of nasal epithelium barrier functions in patients with 
      nasopharyngeal carcinoma post chemo-radiotherapy.
PG  - 116-125
LID - S0167-8140(20)30172-9 [pii]
LID - 10.1016/j.radonc.2020.03.038 [doi]
AB  - BACKGROUND AND PURPOSE: Chronic and recurrent upper respiratory tract infection 
      and inflammation is common in patients with nasopharyngeal carcinoma (NPC) post 
      chemo-radiotherapy (CRT). Whether it is due to intrinsic (e.g., host-defense 
      mechanisms of the epithelium), epigenetic or extrinsic factors is not fully 
      understood. MATERIALS AND METHODS: Tissue biopsies of the middle turbinate (MT) 
      and inferior turbinate (IT) from NPC patients after CRT (mean of 3 years, n = 39) 
      were compared with the IT biopsies from healthy subjects (n = 44). The epithelial 
      ultrastructure was examined by transmission electron microscope (TEM). mRNA and 
      protein expressions of epithelial stem/progenitor cells markers, as well markers 
      of cell proliferation and differentiation markers was analyzed. RESULTS: Abnormal 
      epithelial architecture was observed in all tissue samples of NPC patients. 
      Significantly decreased expression levels of mRNA and protein levels for p63 
      (basal cells), Ki67 (cell proliferation), p63(+)/KRT5(+) (epithelial 
      stem/progenitor cells), MUC5AC and MUC5B (secretary proteins from goblet cells), 
      alpha-tubulin, beta-tubulin and TAp73 (ciliated cells), DNAH5 and DNAI1 and 
      RSPH4A (microtubule assemblies of motile cilia), FOXJ1 and CP110 
      (ciliogenesis-associated markers) were evident in MT and IT biopsies from NPC 
      patients when compared to healthy controls. CONCLUSION: CRT causes long-term 
      defects of epithelial barrier functions and increases the susceptibility of these 
      patients to upper respiratory tract infection and inflammation.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Zhou, Suizi
AU  - Zhou S
AD  - Department of Otolaryngology, Zhujiang Hospital, Southern Medical University, 
      Guangzhou, China.
FAU - Huang, Hongming
AU  - Huang H
AD  - The Second School of Clinical Medicine, Southern Medical University, Guangzhou, 
      China; Department of Otolaryngology&Head and Neck Surgery, Guangdong Provincial 
      People's Hospital and Guangdong Academy of Medical Sciences, China.
FAU - Chen, Qianmin
AU  - Chen Q
AD  - Department of Otolaryngology, Zhujiang Hospital, Southern Medical University, 
      Guangzhou, China.
FAU - Tan, Kai Sen
AU  - Tan KS
AD  - Department of Otolaryngology, National University of Singapore, National 
      University Health System, Singapore.
FAU - Zhu, Zhenchao
AU  - Zhu Z
AD  - Department of Otolaryngology, Zhujiang Hospital, Southern Medical University, 
      Guangzhou, China.
FAU - Peng, Yang
AU  - Peng Y
AD  - The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 
      Institute of Respiratory Health, State Key Laboratory of Respiratory Disease, 
      National Clinical Research Center for Respiratory Disease, Guangzhou Medical 
      University, Guangzhou, China.
FAU - Ong, Hsiao Hui
AU  - Ong HH
AD  - Department of Otolaryngology, National University of Singapore, National 
      University Health System, Singapore.
FAU - Liu, Jing
AU  - Liu J
AD  - Department of Otolaryngology, National University of Singapore, National 
      University Health System, Singapore.
FAU - Xu, Minghong
AU  - Xu M
AD  - Department of Otolaryngology, Zhujiang Hospital, Southern Medical University, 
      Guangzhou, China.
FAU - Gao, Junxiao
AU  - Gao J
AD  - Department of Otolaryngology, Zhujiang Hospital, Southern Medical University, 
      Guangzhou, China.
FAU - Chen, Hailing
AU  - Chen H
AD  - Department of Otolaryngology, Zhujiang Hospital, Southern Medical University, 
      Guangzhou, China.
FAU - Tay, Joshua K
AU  - Tay JK
AD  - Department of Otolaryngology, National University of Singapore, National 
      University Health System, Singapore.
FAU - Qiu, Qianhui
AU  - Qiu Q
AD  - Department of Otolaryngology, Zhujiang Hospital, Southern Medical University, 
      Guangzhou, China. Electronic address: qiuqianhui@hotmail.com.
FAU - Wang, De-Yun
AU  - Wang DY
AD  - Department of Otolaryngology, National University of Singapore, National 
      University Health System, Singapore. Electronic address: entwdy@nus.edu.sg.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200406
PL  - Ireland
TA  - Radiother Oncol
JT  - Radiotherapy and oncology : journal of the European Society for Therapeutic 
      Radiology and Oncology
JID - 8407192
SB  - IM
MH  - Chemoradiotherapy
MH  - *Epithelial Cells
MH  - Humans
MH  - Nasal Mucosa
MH  - Nasopharyngeal Carcinoma/genetics
MH  - *Nasopharyngeal Neoplasms/genetics
OTO - NOTNLM
OT  - Chemo-radiotherapy
OT  - Differentiation
OT  - Epithelium
OT  - NESPCs
OT  - NPC
OT  - Proliferation
EDAT- 2020/05/01 06:00
MHDA- 2021/04/15 06:00
CRDT- 2020/05/01 06:00
PHST- 2019/12/25 00:00 [received]
PHST- 2020/03/23 00:00 [revised]
PHST- 2020/03/27 00:00 [accepted]
PHST- 2020/05/01 06:00 [pubmed]
PHST- 2021/04/15 06:00 [medline]
PHST- 2020/05/01 06:00 [entrez]
AID - S0167-8140(20)30172-9 [pii]
AID - 10.1016/j.radonc.2020.03.038 [doi]
PST - ppublish
SO  - Radiother Oncol. 2020 Jul;148:116-125. doi: 10.1016/j.radonc.2020.03.038. Epub 
      2020 Apr 6.