PMID- 32355441
OWN - NLM
STAT- MEDLINE
DCOM- 20210510
LR  - 20211204
IS  - 1090-0535 (Electronic)
IS  - 1090-0535 (Linking)
VI  - 26
DP  - 2020
TI  - Effect of vitamin B(12) supplementation on retinal lesions in diabetic rats.
PG  - 311-325
AB  - PURPOSE: Diabetic retinopathy (DR) is the most common complication of diabetes 
      involving microvasculature and neuronal alterations in the retina. Previously, we 
      reported that vitamin B(12) deficiency could be an independent risk factor for DR 
      in humans. However, the effect of vitamin B(12) supplementation in experimental 
      DR is unknown. Thus, in this study, we investigated the impact of dietary 
      supplementation of vitamin B(12) on retinal changes in diabetic rats. METHODS: 
      Diabetes was induced in 2-month-old Sprague-Dawley rats and maintained for 4 
      months. One group of diabetic rats were fed normal levels of vitamin B(12), and 
      one group double the quantity of vitamin B(12) (50 microg/kg diet). Vitamin B(12) and 
      homocysteine levels in the plasma were analyzed with radioimmunoassay (RIA) and 
      high-performance liquid chromatography (HPLC), respectively. At the end of 4 
      months of experimentation, the eyeballs were collected. Retinal changes were 
      analyzed with hematoxylin and eosin (H&E) staining, immunoblotting, and 
      immunofluorescence methods. RESULTS: Dietary supplementation of vitamin B(12) had 
      no effect on food intake, bodyweight, fasting blood glucose, and plasma 
      homocysteine levels in the diabetic rats. However, vitamin B(12) supplementation 
      prevented loss of rhodopsin, and overexpression of VEGF, and completely prevented 
      overexpression of HIF1alpha, GFAP, and endoplasmic reticulum (ER) stress markers 
      (GRP78, ATF6alpha, XBP1, CHOP, and caspase 12) in the diabetic rat retina. Further, 
      vitamin B(12) ameliorated apoptosis in the retina as shown with terminal 
      deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) and prevented retinal 
      thinning. CONCLUSIONS: Vitamin B(12) supplementation of diabetic rats appeared to 
      be beneficial by circumventing retinal hypoxia, VEGF overexpression, and ER 
      stress-mediated cell death in the retina. The present study adds another 
      potential therapeutic strategy of vitamin B(12) in diabetes.
CI  - Copyright (c) 2020 Molecular Vision.
FAU - Reddy, S Sreenivasa
AU  - Reddy SS
AD  - Biochemistry Division, National Institute of Nutrition, Hyderabad, India.
FAU - Prabhakar, Y K
AU  - Prabhakar YK
AD  - Biochemistry Division, National Institute of Nutrition, Hyderabad, India.
FAU - Kumar, Ch Uday
AU  - Kumar CU
AD  - Biochemistry Division, National Institute of Nutrition, Hyderabad, India.
FAU - Reddy, P Yadagiri
AU  - Reddy PY
AD  - Biochemistry Division, National Institute of Nutrition, Hyderabad, India.
FAU - Reddy, G Bhanuprakash
AU  - Reddy GB
AD  - Biochemistry Division, National Institute of Nutrition, Hyderabad, India.
LA  - eng
PT  - Journal Article
DEP - 20200424
PL  - United States
TA  - Mol Vis
JT  - Molecular vision
JID - 9605351
RN  - 0 (Activating Transcription Factor 6)
RN  - 0 (Atf6 protein, rat)
RN  - 0 (Blood Glucose)
RN  - 0 (Ddit3 protein, rat)
RN  - 0 (Endoplasmic Reticulum Chaperone BiP)
RN  - 0 (GFAP protein, rat)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (HSPA5 protein, human)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (X-Box Binding Protein 1)
RN  - 0 (Xbp1 protein, rat)
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - 147336-12-7 (Transcription Factor CHOP)
RN  - 9009-81-8 (Rhodopsin)
RN  - EC 3.4.22.- (Caspase 12)
RN  - P6YC3EG204 (Vitamin B 12)
SB  - IM
MH  - Activating Transcription Factor 6/blood
MH  - Animals
MH  - Apoptosis/*drug effects/physiology
MH  - Blood Glucose/drug effects
MH  - Body Weight/drug effects
MH  - Caspase 12/blood
MH  - Chromatography, High Pressure Liquid
MH  - Diabetes Mellitus, Experimental/*blood/drug therapy/metabolism
MH  - Diabetic Retinopathy/*blood/*diet therapy
MH  - Endoplasmic Reticulum Chaperone BiP
MH  - Endoplasmic Reticulum Stress/*drug effects/physiology
MH  - Glial Fibrillary Acidic Protein/blood
MH  - Heat-Shock Proteins/blood
MH  - Homocysteine/blood
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/blood
MH  - Immunohistochemistry
MH  - Male
MH  - Radioimmunoassay
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Rhodopsin/blood
MH  - Transcription Factor CHOP/blood
MH  - Vascular Endothelial Growth Factor A/blood
MH  - Vitamin B 12/*administration & dosage/blood
MH  - X-Box Binding Protein 1/blood
PMC - PMC7190579
EDAT- 2020/05/02 06:00
MHDA- 2021/05/11 06:00
PMCR- 2020/01/01
CRDT- 2020/05/02 06:00
PHST- 2020/01/09 00:00 [received]
PHST- 2020/04/22 00:00 [accepted]
PHST- 2020/05/02 06:00 [entrez]
PHST- 2020/05/02 06:00 [pubmed]
PHST- 2021/05/11 06:00 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - 26 [pii]
PST - epublish
SO  - Mol Vis. 2020 Apr 24;26:311-325. eCollection 2020.