PMID- 32357526
OWN - NLM
STAT- MEDLINE
DCOM- 20210215
LR  - 20210215
IS  - 2072-6643 (Electronic)
IS  - 2072-6643 (Linking)
VI  - 12
IP  - 5
DP  - 2020 Apr 26
TI  - Protective Effects of Myo-Inositol and Selenium on Cadmium-Induced Thyroid 
      Toxicity in Mice.
LID - 10.3390/nu12051222 [doi]
LID - 1222
AB  - Cadmium (Cd) damages the thyroid gland. We evaluated the effects of myo-inositol 
      (MI), seleno-L-methionine (Se) or their combination on the thyroids of mice 
      simultaneously administered with Cd chloride (CdCl(2)). Eighty-four male mice 
      were divided into 12 groups (seven mice each). Six groups (controls) were treated 
      with 0.9% NaCl (vehicle), Se (0.2 mg/kg/day), Se (0.4 mg/kg/day), MI (360 
      mg/kg/day), MI+Se (0.2 mg/kg) and MI+Se (0.4 mg/kg). The other six groups were 
      treated with CdCl(2) (2 mg/kg), CdCl(2)+MI, CdCl(2)+Se (0.2 mg/kg), CdCl(2)+Se 
      (0.4 mg/kg), CdCl(2)+MI+Se (0.2 mg/kg) and CdCl(2)+MI+Se (0.4 mg/kg). An 
      additional group of CdCl(2)-challenged animals (n= 7) was treated with 
      resveratrol (20 mg/kg), an effective and potent antioxidant. All treatments 
      lasted 14 days. After sacrifice, the thyroids were evaluated histologically and 
      immunohistochemically. CdCl(2) reduced the follicular area, increased the 
      epithelial height, stroma, and cells expressing monocyte chemoattractant 
      protein-1 (MCP-1) and C-X-C motif chemokine 10 (CXCL10). CdCl(2)+Se at 0.2/0.4 
      mg/kg insignificantly reversed the follicular and stromal structure, and 
      significantly decreased the number of MCP-1 and CXCL10-positive cells. CdCl(2)+MI 
      significantly reversed the thyroid structure and further decreased the number of 
      MCP-1 and CXCL10-positive cells. CdCl(2)+MI+Se, at both doses, brought all 
      indices to those of CdCl(2)-untreated mice. MI, particularly in association with 
      Se, defends mice from Cd-induced damage. The efficacy of this combination was 
      greater than that of resveratrol, at least when using the follicular structure as 
      a read-out for a comparison. We suggest that the use of these nutraceuticals, 
      more specifically the combination of MI plus SE, can protect the thyroid of 
      Cd-exposed subjects.
FAU - Benvenga, Salvatore
AU  - Benvenga S
AD  - Department of Clinical and Experimental Medicine, University of Messina, 98125 
      Messina, Italy.
FAU - Marini, Herbert R
AU  - Marini HR
AD  - Department of Clinical and Experimental Medicine, University of Messina, 98125 
      Messina, Italy.
FAU - Micali, Antonio
AU  - Micali A
AD  - Department of Biomedical and Dental Sciences and Morphofunctional Imaging, 
      University of Messina, 98125 Messina, Italy.
FAU - Freni, Jose
AU  - Freni J
AD  - Department of Biomedical and Dental Sciences and Morphofunctional Imaging, 
      University of Messina, 98125 Messina, Italy.
FAU - Pallio, Giovanni
AU  - Pallio G
AD  - Department of Clinical and Experimental Medicine, University of Messina, 98125 
      Messina, Italy.
FAU - Irrera, Natasha
AU  - Irrera N
AD  - Department of Clinical and Experimental Medicine, University of Messina, 98125 
      Messina, Italy.
FAU - Squadrito, Francesco
AU  - Squadrito F
AD  - Department of Clinical and Experimental Medicine, University of Messina, 98125 
      Messina, Italy.
FAU - Altavilla, Domenica
AU  - Altavilla D
AD  - Department of Biomedical and Dental Sciences and Morphofunctional Imaging, 
      University of Messina, 98125 Messina, Italy.
FAU - Antonelli, Alessandro
AU  - Antonelli A
AD  - Department of Clinical and Experimental Medicine, University of Pisa, 56126 Pisa, 
      Italy.
FAU - Ferrari, Silvia Martina
AU  - Ferrari SM
AD  - Department of Clinical and Experimental Medicine, University of Pisa, 56126 Pisa, 
      Italy.
FAU - Fallahi, Poupak
AU  - Fallahi P
AD  - Department of Clinical and Experimental Medicine, University of Pisa, 56126 Pisa, 
      Italy.
FAU - Puzzolo, Domenico
AU  - Puzzolo D
AD  - Department of Biomedical and Dental Sciences and Morphofunctional Imaging, 
      University of Messina, 98125 Messina, Italy.
FAU - Minutoli, Letteria
AU  - Minutoli L
AD  - Department of Clinical and Experimental Medicine, University of Messina, 98125 
      Messina, Italy.
LA  - eng
PT  - Journal Article
DEP - 20200426
PL  - Switzerland
TA  - Nutrients
JT  - Nutrients
JID - 101521595
RN  - 0 (Antioxidants)
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CXCL10)
RN  - 0 (Cxcl10 protein, mouse)
RN  - 4L6452S749 (Inositol)
RN  - H6241UJ22B (Selenium)
RN  - J6K4F9V3BA (Cadmium Chloride)
RN  - Q369O8926L (Resveratrol)
SB  - IM
MH  - Animals
MH  - Antioxidants
MH  - Cadmium Chloride/*toxicity
MH  - Chemokine CCL2/metabolism
MH  - Chemokine CXCL10/metabolism
MH  - *Dietary Supplements
MH  - Immunohistochemistry
MH  - Inositol/*administration & dosage/*pharmacology
MH  - Male
MH  - Mice, Inbred C57BL
MH  - Resveratrol/administration & dosage/pharmacology
MH  - Selenium/*administration & dosage/*pharmacology
MH  - Thyroid Gland/*drug effects/*metabolism/pathology
PMC - PMC7282027
OTO - NOTNLM
OT  - CXCL10
OT  - MCP-1
OT  - cadmium
OT  - myo-inositol
OT  - nutraceuticals
OT  - seleno-L-methionine
OT  - thyroid
COIS- S.B. has been an invited speaker for LO.LI. Pharma, which provided us with pure 
      MI, but had no role in the design of the study; in the collection, analyses, or 
      interpretation of data; in the writing of the manuscript, or in the decision to 
      publish the results. All the other authors declare no conflict of interest.
EDAT- 2020/05/03 06:00
MHDA- 2021/02/16 06:00
PMCR- 2020/05/01
CRDT- 2020/05/03 06:00
PHST- 2020/03/25 00:00 [received]
PHST- 2020/04/17 00:00 [revised]
PHST- 2020/04/23 00:00 [accepted]
PHST- 2020/05/03 06:00 [entrez]
PHST- 2020/05/03 06:00 [pubmed]
PHST- 2021/02/16 06:00 [medline]
PHST- 2020/05/01 00:00 [pmc-release]
AID - nu12051222 [pii]
AID - nutrients-12-01222 [pii]
AID - 10.3390/nu12051222 [doi]
PST - epublish
SO  - Nutrients. 2020 Apr 26;12(5):1222. doi: 10.3390/nu12051222.