PMID- 32357618
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200504
IS  - 1559-0097 (Electronic)
IS  - 1046-3976 (Linking)
VI  - 2
IP  - 1
DP  - 1991 Mar
TI  - Immunohistochemical study of crooke's hyaline change.
PG  - 12-15
LID - 10.1007/BF02915321 [doi]
AB  - Crooke's hyaline change of the human pituitary gland appears as an 
      intracytoplasmic accumulation of fine filaments under electron microscopy. This 
      study was attempted to identify the fine filaments by immunohistochemical 
      methods. Twenty-eight postmortem, formalin-fixed or chrome-alum-fixed, 
      paraffin-embedded pituitary glands revealing unequivocal Crooke's hyaline change 
      on hematoxylin and eosin stain were selected for this study. To demonstrate 
      Crooke's cells and fine filaments simultaneously, mirror image sections were 
      sliced and stained with the following monoclonal antibodies using an 
      avidin-biotin-peroxidase complex method: an antibody against synthesized 
      adrenocorticotropic hormone 1-24, human cytokeratins (55-57 kilodalton [kd] and 
      68 kd), porcine vimentin (57 kd), porcine desmin (53 kd), bovine neurofilaments 
      (70, 160, and 210 kd), human glial fibrilfary acidic protein (GFAP) (56 kd), and 
      chicken actin. Crooke's cells showed a variable intensity of cytoplasmic staining 
      for 55- to 57-kd cytokeratins, from focal to more even and intense staining 
      revealing a characteristic wide brown ring around the nucleus or beneath the cell 
      membrane. The most severely affected cells were totally replaced by dark brown 
      reaction products with no secretory granules detectable in the cytoplasm. 
      However, 68-kd cytokeratin could not be unequivocally demonstrated. Crooke's 
      cells were all negative for vimentin, desmin, neurofilaments, GFAP, and actin. 
      Thus far, it could be concluded that Crooke's hyaline change was composed of 
      intermediate-subunit molecular weight cytokeratins that are normal constituents 
      of the ACTH cell.
FAU - Uei, Yoshio
AU  - Uei Y
AD  - Cytology Section, Clinical Laboratory, National Cancer Center Hospital, Japan.
FAU - Kanzaki, Masaki
AU  - Kanzaki M
AD  - Clinical Laboratory, Kamo Hospital, Japan.
FAU - Ohta, Toshio
AU  - Ohta T
AD  - Clinical Laboratory, Yamada Red Cross Hospital, Japan.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Endocr Pathol
JT  - Endocrine pathology
JID - 9009288
SB  - IM
OTO - NOTNLM
OT  - ACTH Cell
OT  - Fine Filament
OT  - Human Pituitary Gland
OT  - Human Stratum Corneum
OT  - Normal Pituitary Gland
EDAT- 1991/03/01 00:00
MHDA- 1991/03/01 00:01
CRDT- 2020/05/03 06:00
PHST- 2020/05/03 06:00 [entrez]
PHST- 1991/03/01 00:00 [pubmed]
PHST- 1991/03/01 00:01 [medline]
AID - 10.1007/BF02915321 [pii]
AID - 10.1007/BF02915321 [doi]
PST - ppublish
SO  - Endocr Pathol. 1991 Mar;2(1):12-15. doi: 10.1007/BF02915321.