PMID- 32359647
OWN - NLM
STAT- MEDLINE
DCOM- 20210623
LR  - 20210623
IS  - 1557-7988 (Electronic)
IS  - 0076-6879 (Linking)
VI  - 637
DP  - 2020
TI  - DNA recognition by retinoic acid nuclear receptors.
PG  - 235-260
LID - S0076-6879(20)30102-6 [pii]
LID - 10.1016/bs.mie.2020.03.001 [doi]
AB  - Retinoic acid receptors (RARs) heterodimerize with retinoid X receptors (RXRs) to 
      regulate gene expression. The heterodimer recognizes the genome via a large and 
      diverse repertoire of DNA response elements. Assessing the binding mode of RAR 
      and RXR with various DNA response elements is important for understanding how 
      they select their binding site and how DNA sequence and topology allosterically 
      regulate RAR function. A number of complementary assays are often employed for 
      analysis of the binding mode. To biochemically and structurally characterize RAR 
      and RXR-DNA complexes, we describe how to express and purify RAR and RXR-DNA 
      binding domains (DBDs) and multidomain constructs. We also describe the use of 
      electrospray ionization mass spectrometry (ESI MS) and isothermal titration 
      calorimetry (ITC) that give information about stoichiometry and binding affinity, 
      as well as our approaches for co-crystallization of RAR and RXR DBDs with DNA.
CI  - (c) 2020 Elsevier Inc. All rights reserved.
FAU - Peluso-Iltis, Carole
AU  - Peluso-Iltis C
AD  - Institut de Genetique et de Biologie Moleculaire et Cellulaire (IGBMC), Illkirch, 
      France; Centre National de la Recherche Scientifique, Illkirch, France; Institut 
      National de la Sante et de la Recherche Medicale, Illkirch, France; Universite de 
      Strasbourg, Illkirch, France.
FAU - Osz, Judit
AU  - Osz J
AD  - Institut de Genetique et de Biologie Moleculaire et Cellulaire (IGBMC), Illkirch, 
      France; Centre National de la Recherche Scientifique, Illkirch, France; Institut 
      National de la Sante et de la Recherche Medicale, Illkirch, France; Universite de 
      Strasbourg, Illkirch, France.
FAU - Rochel, Natacha
AU  - Rochel N
AD  - Institut de Genetique et de Biologie Moleculaire et Cellulaire (IGBMC), Illkirch, 
      France; Centre National de la Recherche Scientifique, Illkirch, France; Institut 
      National de la Sante et de la Recherche Medicale, Illkirch, France; Universite de 
      Strasbourg, Illkirch, France. Electronic address: rochel@igbmc.fr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200331
PL  - United States
TA  - Methods Enzymol
JT  - Methods in enzymology
JID - 0212271
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Receptors, Retinoic Acid)
RN  - 0 (Retinoid X Receptors)
RN  - 5688UTC01R (Tretinoin)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - DNA
MH  - *DNA-Binding Proteins
MH  - *Receptors, Retinoic Acid/genetics
MH  - Retinoid X Receptors/genetics
MH  - Tretinoin
OTO - NOTNLM
OT  - Biophysics
OT  - Crystallization
OT  - DNA recognition
OT  - ESI MS
OT  - ITC
OT  - Protein expression and production
OT  - RAR-RXR-DNA complex
EDAT- 2020/05/04 06:00
MHDA- 2021/06/24 06:00
CRDT- 2020/05/04 06:00
PHST- 2020/05/04 06:00 [entrez]
PHST- 2020/05/04 06:00 [pubmed]
PHST- 2021/06/24 06:00 [medline]
AID - S0076-6879(20)30102-6 [pii]
AID - 10.1016/bs.mie.2020.03.001 [doi]
PST - ppublish
SO  - Methods Enzymol. 2020;637:235-260. doi: 10.1016/bs.mie.2020.03.001. Epub 2020 Mar 
      31.