PMID- 32360461
OWN - NLM
STAT- Publisher
LR  - 20240227
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
DP  - 2020 Apr 29
TI  - Natural soluble epoxide hydrolase inhibitors from Inula helenium and their 
      interactions with soluble epoxide hydrolase.
LID - S0141-8130(20)33090-7 [pii]
LID - 10.1016/j.ijbiomac.2020.04.227 [doi]
AB  - The inhibition of soluble epoxide hydrolase (sEH) is regarded as a promising 
      therapeutic approach to treat inflammation and its related disorders. In present 
      work, we investigated inhibitory effects of forty-nine kinds of traditional 
      Chinese medicines against sEH. Inula helenium showed significant inhibitory 
      effect against sEH, and the extract of I. helenium were isolated to obtain eight 
      compounds, including 4H-tomentosin (1), xanthalongin (2), and linoleic acid (3), 
      8-hydroxy-9-isobutyryloxy-10(2)-methylbutyrylthymol (4), dehydrocostus lactone 
      (5), alantolactone (6), costunolide (7), and isoalantolactone (8). Among them, 
      4H-tomentosin (1), xanthalongin (2), and linoleic acid (3) showed significantly 
      inhibitory activities on sEH with half maximal inhibitory concentration (IC(50)) 
      from 5.88 +/- 0.97 muM to 11.63 +/- 0.58 muM. The inhibition kinetics suggested that 
      4H-tomentosin (1) and xanthalongin (2) were mixed-competitive type inhibitors 
      with inhibition constant (Ki) values of 7.02 and 6.57 muM, respectively, and 
      linoleic acid (3) was a competitive type inhibitor with a Ki values of 3.52 muM. 
      The potential interactions of 4H-tomentosin (1), xanthalongin (2), and linoleic 
      acid (3) with sEH were analyzed by molecular docking, which indicated that these 
      bioactive compounds had interactions with key amino acid residues Tyr343, Ile363, 
      Tyr383, and His524.
CI  - Copyright (c) 2018. Published by Elsevier B.V.
FAU - He, Xin
AU  - He X
AD  - College of Pharmacy, College (Institute) of Integrative Medicine, Dalian Medical 
      University, Dalian, China.
FAU - Zhao, Wen-Yu
AU  - Zhao WY
AD  - College of Pharmacy, College (Institute) of Integrative Medicine, Dalian Medical 
      University, Dalian, China.
FAU - Shao, Bo
AU  - Shao B
AD  - College of Pharmacy, College (Institute) of Integrative Medicine, Dalian Medical 
      University, Dalian, China.
FAU - Zhang, Bao-Jing
AU  - Zhang BJ
AD  - College of Pharmacy, College (Institute) of Integrative Medicine, Dalian Medical 
      University, Dalian, China.
FAU - Liu, Tian-Tian
AU  - Liu TT
AD  - College of Pharmacy, College (Institute) of Integrative Medicine, Dalian Medical 
      University, Dalian, China.
FAU - Sun, Cheng-Peng
AU  - Sun CP
AD  - College of Pharmacy, College (Institute) of Integrative Medicine, Dalian Medical 
      University, Dalian, China. Electronic address: suncp146@163.com.
FAU - Huang, Hui-Lian
AU  - Huang HL
AD  - Laboratory of Modern Preparation of Traditional Chinese Medicine, Ministry of 
      Education, Jiangxi University of Traditional Chinese Medicine, Nanchang, China.
FAU - Wu, Jia-Rong
AU  - Wu JR
AD  - Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical 
      University, Xuzhou, China.
FAU - Liang, Jia-Hao
AU  - Liang JH
AD  - Zhendong Pharmaceutical Research Institute Co. Ltd., Changzhi, China; Jiangsu Key 
      Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, 
      Xuzhou, China.
FAU - Ma, Xiao-Chi
AU  - Ma XC
AD  - College of Pharmacy, College (Institute) of Integrative Medicine, Dalian Medical 
      University, Dalian, China; Jiangsu Key Laboratory of New Drug Research and 
      Clinical Pharmacy, Xuzhou Medical University, Xuzhou, China. Electronic address: 
      maxiaochi2019@163.com.
LA  - eng
PT  - Journal Article
DEP - 20200429
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
SB  - IM
OTO - NOTNLM
OT  - Kinetics
OT  - Molecular stimulation
OT  - Soluble epoxide hydrolase
COIS- Declaration of competing interest The authors declare no competing financial 
      interest.
EDAT- 2020/05/04 06:00
MHDA- 2020/05/04 06:00
CRDT- 2020/05/04 06:00
PHST- 2020/02/06 00:00 [received]
PHST- 2020/03/15 00:00 [revised]
PHST- 2020/04/25 00:00 [accepted]
PHST- 2020/05/04 06:00 [entrez]
PHST- 2020/05/04 06:00 [pubmed]
PHST- 2020/05/04 06:00 [medline]
AID - S0141-8130(20)33090-7 [pii]
AID - 10.1016/j.ijbiomac.2020.04.227 [doi]
PST - aheadofprint
SO  - Int J Biol Macromol. 2020 Apr 29:S0141-8130(20)33090-7. doi: 
      10.1016/j.ijbiomac.2020.04.227.