PMID- 32363436
OWN - NLM
STAT- MEDLINE
DCOM- 20210120
LR  - 20210120
IS  - 1435-604X (Electronic)
IS  - 0268-8921 (Linking)
VI  - 36
IP  - 1
DP  - 2021 Feb
TI  - Conditioned media from blue light-emitting diode-exposed fibroblasts have an 
      anti-inflammatory effect in vitro.
PG  - 99-109
LID - 10.1007/s10103-020-03018-x [doi]
AB  - We have previously reported the protective effects of blue light-emitting diode 
      (BLED)-stimulated cell metabolites on cell injury. To further examine the effect 
      of conditioned media (CM) derived from BLED (5 J/cm(2))-exposed human normal 
      fibroblasts (CMBL5) for clinical application, we have used the choline chloride 
      and phenol red-free media and then concentrated CMBL5 using a centrifugal filter 
      unit. The collected CMBL5-lower part (CMBL5-LO) has evaluated the inflammatory 
      protein expression profile in LPS-stimulated RAW264.7 cells. Comprehensive 
      metabolomic profiling of CMBL5-LO was carried out using hybrid tandem mass 
      spectrometry. Treatment with CMBL5-LO showed the cytoprotective effect on 
      apoptotic cell death, but rather increased apoptotic cells after treatment with 
      CMBL5-upper part (CMBL5-UP). In addition, CMBL5-LO inhibited several 
      chemo-attractants, including interleukin (IL)-6, macrophage inflammatory protein 
      (MIP)-2, chemokine (C-C motif) ligand 5 (CCL5), granulocyte colony-stimulating 
      factor (GCSF), and monocyte chemoattractant protein-1 (MCP-1) expression. 
      Pro-inflammatory nitric oxide was decreased after CMBL5-LO treatment, but not by 
      CMBL5-UP treatment. Interestingly, treatment with CMBL5-LO stimulated expression 
      of heme oxygenase-1, indicating its anti-inflammatory property. Most endoplasmic 
      reticulum (ER) stress proteins except for transcription factor C/EBP homologous 
      protein (CHOP) were highly expressed after irradiation with BLED in cells. 
      Further studies are needed to examine the precise mechanism by CMBL5-LO in cells.
FAU - Oh, Phil-Sun
AU  - Oh PS
AD  - Department of Nuclear Medicine, Molecular Imaging & Therapeutic Medicine Research 
      Center, Jeonbuk Regional Cyclotron Research Center & Biotracer Imaging Lab, 
      Research Institute of Clinical Medicine of Jeonbuk National University and 
      Biomedical Research Institute of Jeonbuk National University Hospital, 20, 
      Geonji-ro, Duckjin-gu, Jeonju-si, Jeollabuk-do, 561-803, South Korea.
FAU - Kim, Eun-Mi
AU  - Kim EM
AD  - Department of Nuclear Medicine, Molecular Imaging & Therapeutic Medicine Research 
      Center, Jeonbuk Regional Cyclotron Research Center & Biotracer Imaging Lab, 
      Research Institute of Clinical Medicine of Jeonbuk National University and 
      Biomedical Research Institute of Jeonbuk National University Hospital, 20, 
      Geonji-ro, Duckjin-gu, Jeonju-si, Jeollabuk-do, 561-803, South Korea.
FAU - Lim, SeokTae
AU  - Lim S
AD  - Department of Nuclear Medicine, Molecular Imaging & Therapeutic Medicine Research 
      Center, Jeonbuk Regional Cyclotron Research Center & Biotracer Imaging Lab, 
      Research Institute of Clinical Medicine of Jeonbuk National University and 
      Biomedical Research Institute of Jeonbuk National University Hospital, 20, 
      Geonji-ro, Duckjin-gu, Jeonju-si, Jeollabuk-do, 561-803, South Korea.
FAU - Sohn, Myung-Hee
AU  - Sohn MH
AD  - Department of Nuclear Medicine, Molecular Imaging & Therapeutic Medicine Research 
      Center, Jeonbuk Regional Cyclotron Research Center & Biotracer Imaging Lab, 
      Research Institute of Clinical Medicine of Jeonbuk National University and 
      Biomedical Research Institute of Jeonbuk National University Hospital, 20, 
      Geonji-ro, Duckjin-gu, Jeonju-si, Jeollabuk-do, 561-803, South Korea.
FAU - Jeong, Hwan-Jeong
AU  - Jeong HJ
AD  - Department of Nuclear Medicine, Molecular Imaging & Therapeutic Medicine Research 
      Center, Jeonbuk Regional Cyclotron Research Center & Biotracer Imaging Lab, 
      Research Institute of Clinical Medicine of Jeonbuk National University and 
      Biomedical Research Institute of Jeonbuk National University Hospital, 20, 
      Geonji-ro, Duckjin-gu, Jeonju-si, Jeollabuk-do, 561-803, South Korea. 
      jayjeong@jbnu.ac.kr.
LA  - eng
GR  - 2016R1D1A1B03932595/National Research Foundation of Korea/
GR  - HI15C1529/Korea Health Industry Development Institute/Republic of Korea
PT  - Journal Article
DEP - 20200504
PL  - England
TA  - Lasers Med Sci
JT  - Lasers in medical science
JID - 8611515
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Chemokine CXCL2)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Protective Agents)
RN  - 31C4KY9ESH (Nitric Oxide)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Apoptosis/drug effects
MH  - Cell Line
MH  - Chemokine CXCL2/metabolism
MH  - Color
MH  - Culture Media, Conditioned/*pharmacology
MH  - Cytokines/metabolism
MH  - Endoplasmic Reticulum Stress/drug effects
MH  - Fibroblasts/metabolism/*radiation effects
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Interleukin-6/metabolism
MH  - *Light
MH  - Lipopolysaccharides/pharmacology
MH  - Mice
MH  - Nitric Oxide/biosynthesis
MH  - Protective Agents/pharmacology
MH  - RAW 264.7 Cells
MH  - Up-Regulation/drug effects
OTO - NOTNLM
OT  - CMBL5-LO
OT  - ER stress
OT  - Inflammation
OT  - Light-emitting diode
EDAT- 2020/05/05 06:00
MHDA- 2021/01/21 06:00
CRDT- 2020/05/05 06:00
PHST- 2019/12/11 00:00 [received]
PHST- 2020/04/06 00:00 [accepted]
PHST- 2020/05/05 06:00 [pubmed]
PHST- 2021/01/21 06:00 [medline]
PHST- 2020/05/05 06:00 [entrez]
AID - 10.1007/s10103-020-03018-x [pii]
AID - 10.1007/s10103-020-03018-x [doi]
PST - ppublish
SO  - Lasers Med Sci. 2021 Feb;36(1):99-109. doi: 10.1007/s10103-020-03018-x. Epub 2020 
      May 4.