PMID- 32363688 OWN - NLM STAT- MEDLINE DCOM- 20210429 LR - 20211204 IS - 1582-4934 (Electronic) IS - 1582-1838 (Print) IS - 1582-1838 (Linking) VI - 24 IP - 12 DP - 2020 Jun TI - Regulation of laryngeal squamous cell cancer progression by the lncRNA RP11-159K7.2/miR-206/DNMT3A axis. PG - 6781-6795 LID - 10.1111/jcmm.15331 [doi] AB - Long non-coding RNAs (lncRNAs), which are longer than 200 nt, have been proved to play a role in promoting or inhibiting cancer progression. The following study investigated the role and underlying mechanisms of lncRNA RP11-159K7.2 in laryngeal squamous cell carcinoma (LSCC) progression. Briefly, in situ hybridization (ISH) and real-time quantitative PCR (RT-qPCR) showed higher expression of RP11-159K7.2 in LSCC tissues and cell lines. Patients with low expression level of RP11-159K7.2 lived longer compared to those with high expression of RP11-159K7.2 (chi(2) = 39.111, ***P < 0.001). Multivariate Cox regression analysis suggested that lncRNA RP11-159K7.2 was an independent prognostic factor for LSCC patients (HR = 2.961, ***P < 0.001). Furthermore, to investigate the potential involvement of RP11-159K7.2 in the development of LSCC, we knocked out the expression of endogenous RP11-159K7.2 in TU-212 cells and AMC-HN-8 cells via CRISPR/Cas9 double vector lentiviral system. RP11-159K7.2 knockout decreased LSCC cell growth and invasion both in vitro and in vivo. Mechanically, we found that RP11-159K7.2 could positively regulate the expression of DNMT3A by sponging miR-206. In addition, a feedback loop was also discovered between DNMT3A and miR-206. To sum up, these findings suggest that lncRNA RP11-159K7.2 could be used as a potential biomarker for prognosis and treatment of LSCC. CI - (c) 2020 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. FAU - Wang, Xin AU - Wang X AD - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University, Harbin, China. FAU - Yu, Boyu AU - Yu B AD - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University, Harbin, China. FAU - Jin, Qianqian AU - Jin Q AD - Department of Otorhinolaryngology, Head and Neck Surgery, Puyang Oilfield General Hospital, Puyang, China. FAU - Zhang, Junyi AU - Zhang J AD - Department of Otorhinolaryngology, Daqing Oilfield General Hospital, Daqing, China. AD - Department of Otolaryngology, Daqing First Hospital, Daqing, China. FAU - Yan, Bingrui AU - Yan B AD - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University, Harbin, China. FAU - Yang, Like AU - Yang L AD - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University, Harbin, China. FAU - Li, Yushan AU - Li Y AD - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University, Harbin, China. FAU - Li, Qiuying AU - Li Q AD - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University, Harbin, China. FAU - Wang, Peng AU - Wang P AD - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University, Harbin, China. FAU - Sun, Chuanhui AU - Sun C AD - Department of Otorhinolaryngology, Head and Neck Surgery, The First Affiliated Hospital, Guizhou University of Traditional Chinese Medicine, Guizhou, China. FAU - Liu, Ming AU - Liu M AD - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University, Harbin, China. FAU - Tian, Linli AU - Tian L AD - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University, Harbin, China. FAU - Sun, Yanan AU - Sun Y AUID- ORCID: 0000-0003-4587-3297 AD - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University, Harbin, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20200504 PL - England TA - J Cell Mol Med JT - Journal of cellular and molecular medicine JID - 101083777 RN - 0 (DNMT3A protein, human) RN - 0 (Dnmt3a protein, mouse) RN - 0 (MIRN206 microRNA, human) RN - 0 (MicroRNAs) RN - 0 (RNA, Long Noncoding) RN - EC 2.1.1.37 (DNA (Cytosine-5-)-Methyltransferases) RN - EC 2.1.1.37 (DNA Methyltransferase 3A) SB - IM MH - Animals MH - Base Sequence MH - CRISPR-Cas Systems/genetics MH - Carcinoma, Squamous Cell/*genetics/*pathology MH - Cell Line, Tumor MH - Cell Proliferation MH - DNA (Cytosine-5-)-Methyltransferases/genetics/*metabolism MH - DNA Methylation/genetics MH - DNA Methyltransferase 3A MH - *Disease Progression MH - Feedback, Physiological MH - Female MH - Gene Expression Regulation, Neoplastic MH - Humans MH - Laryngeal Neoplasms/*genetics/*pathology MH - Male MH - Mice, Inbred BALB C MH - MicroRNAs/genetics/*metabolism MH - Middle Aged MH - Multivariate Analysis MH - Neoplasm Invasiveness MH - Prognosis MH - RNA, Long Noncoding/genetics/*metabolism MH - Up-Regulation/genetics PMC - PMC7299727 OTO - NOTNLM OT - CRISPR/Cas9 OT - DNMT3A OT - laryngeal squamous cell cancer (LSCC) OT - long non-coding RNA OT - microRNA COIS- The authors declare that they have no competing interests. EDAT- 2020/05/05 06:00 MHDA- 2021/04/30 06:00 PMCR- 2020/06/01 CRDT- 2020/05/05 06:00 PHST- 2019/11/07 00:00 [received] PHST- 2020/02/27 00:00 [revised] PHST- 2020/04/12 00:00 [accepted] PHST- 2020/05/05 06:00 [pubmed] PHST- 2021/04/30 06:00 [medline] PHST- 2020/05/05 06:00 [entrez] PHST- 2020/06/01 00:00 [pmc-release] AID - JCMM15331 [pii] AID - 10.1111/jcmm.15331 [doi] PST - ppublish SO - J Cell Mol Med. 2020 Jun;24(12):6781-6795. doi: 10.1111/jcmm.15331. Epub 2020 May 4.