PMID- 32363925
OWN - NLM
STAT- MEDLINE
DCOM- 20210812
LR  - 20231112
IS  - 1555-3892 (Electronic)
IS  - 0963-6897 (Print)
IS  - 0963-6897 (Linking)
VI  - 29
DP  - 2020 Jan-Dec
TI  - Extracellular Vesicles Derived from Mesenchymal Stem Cells Recover Fertility of 
      Premature Ovarian Insufficiency Mice and the Effects on their Offspring.
PG  - 963689720923575
LID - 10.1177/0963689720923575 [doi]
LID - 0963689720923575
AB  - It has been reported that extracellular vesicles (EVs) derived from human 
      umbilical cord mesenchymal stem cells (HUCMSCs) can promote the proliferative and 
      secretive functions of granulosa cells. In vivo study further demonstrated that 
      EVs derived from HUCMSCs can not only promote the angiogenesis of ovarian tissue 
      but also restore the function of an ovary of chemically induced premature ovarian 
      insufficiency (POI) mice. However, no study investigates the effects of HUCMSCs 
      derived EVs on fertility recovery of POI mice and evaluating their offspring. 
      This study investigates the effects of HUCMSCs derived EVs on fertility recovery 
      and the cognitive function of their offspring. A POI model was established by 
      intraperitoneal injection of cyclophosphamide (CTX) and busulfan (BUS), and 
      randomly divided into EVs-transplantation group (a single injection of 150 microg EVs 
      proteins which suspended in 0.1 ml phosphate buffer saline [PBS] via tail vein), 
      POI group (a single injection of 0.1 ml PBS via tail vein), and normal control 
      group (a single injection of 0.1 ml PBS via tail vein without intraperitoneal 
      injection of CTX and BUS). After EVs treatment, not only the ovarian function of 
      POI mice recovered but also the fertility increased with less time to get 
      pregnant, evaluating by in vitro fertilization and mating test. Cognitive 
      behaviors of the offspring were similar among the three groups through the Y-maze 
      test and novel object recognition task. An anti-apoptotic effect was identified 
      through immunohistochemistry, real-time polymerase chain reaction and western 
      blot. These findings indicate that HUCMSCs derived EVs can improve the fertility 
      of POI mice without adverse effects on the cognitive behavior of their offspring, 
      highlighting the potential value of EVs to be a cell-free therapy for patients 
      suffering from POI.
FAU - Liu, Conghui
AU  - Liu C
AD  - Reproductive Medicine Center, 105th Hospital of the People's Liberation Army, 
      Hefei, China.
FAU - Yin, Huiqun
AU  - Yin H
AD  - Reproductive Medicine Center, 105th Hospital of the People's Liberation Army, 
      Hefei, China.
FAU - Jiang, Hong
AU  - Jiang H
AUID- ORCID: 0000-0002-4690-7906
AD  - Reproductive Medicine Center, 105th Hospital of the People's Liberation Army, 
      Hefei, China.
FAU - Du, Xin
AU  - Du X
AD  - Reproductive Medicine Center, 105th Hospital of the People's Liberation Army, 
      Hefei, China.
FAU - Wang, Cunli
AU  - Wang C
AD  - Reproductive Medicine Center, 105th Hospital of the People's Liberation Army, 
      Hefei, China.
FAU - Liu, Yingchun
AU  - Liu Y
AD  - Reproductive Medicine Center, 105th Hospital of the People's Liberation Army, 
      Hefei, China.
FAU - Li, Yu
AU  - Li Y
AD  - Reproductive Medicine Center, 105th Hospital of the People's Liberation Army, 
      Hefei, China.
FAU - Yang, Ziling
AU  - Yang Z
AD  - Reproductive Medicine Center, 105th Hospital of the People's Liberation Army, 
      Hefei, China.
AD  - The First Affiliated Hospital of Anhui Medical University, Hefei, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell Transplant
JT  - Cell transplantation
JID - 9208854
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Disease Models, Animal
MH  - Embryo, Mammalian/pathology
MH  - Extracellular Vesicles/*metabolism
MH  - Female
MH  - *Fertility
MH  - Fertilization in Vitro
MH  - Male
MH  - Mesenchymal Stem Cells/*metabolism
MH  - Mice, Inbred ICR
MH  - Oocytes/metabolism
MH  - Ovary/pathology/physiopathology
MH  - Primary Ovarian Insufficiency/*physiopathology/*therapy
PMC - PMC7586265
OTO - NOTNLM
OT  - extracellular vesicles
OT  - fertility
OT  - in vitro fertilization
OT  - mesenchymal stem cell
OT  - offspring
OT  - premature ovarian insufficiency
COIS- Declaration of Conflicting Interests: The author(s) declared no potential 
      conflicts of interest with respect to the research, authorship, and/or 
      publication of this article.
EDAT- 2020/05/05 06:00
MHDA- 2021/08/13 06:00
PMCR- 2020/05/04
CRDT- 2020/05/05 06:00
PHST- 2020/05/05 06:00 [entrez]
PHST- 2020/05/05 06:00 [pubmed]
PHST- 2021/08/13 06:00 [medline]
PHST- 2020/05/04 00:00 [pmc-release]
AID - 10.1177_0963689720923575 [pii]
AID - 10.1177/0963689720923575 [doi]
PST - ppublish
SO  - Cell Transplant. 2020 Jan-Dec;29:963689720923575. doi: 10.1177/0963689720923575.