PMID- 32364380
OWN - NLM
STAT- MEDLINE
DCOM- 20210416
LR  - 20210521
IS  - 1520-5126 (Electronic)
IS  - 0002-7863 (Print)
IS  - 0002-7863 (Linking)
VI  - 142
IP  - 20
DP  - 2020 May 20
TI  - Protein-Protein Cross-Coupling via Palladium-Protein Oxidative Addition Complexes 
      from Cysteine Residues.
PG  - 9124-9129
LID - 10.1021/jacs.0c03143 [doi]
AB  - Few chemical methods exist for the covalent conjugation of two proteins. We 
      report the preparation of site-specific protein-protein conjugates that arise 
      from the sequential cross-coupling of cysteine residues on two different 
      proteins. The method involves the synthesis of stable palladium-protein oxidative 
      addition complexes (Pd-protein OACs), a process that converts nucleophilic 
      cysteine residues into an electrophilic S-aryl-Pd-X unit by taking advantage of 
      an intramolecular oxidative addition strategy. This process is demonstrated on 
      proteins up to 83 kDa in size and can be conveniently carried out in water and 
      open to air. The resulting Pd-protein OACs can cross-couple with other 
      thiol-containing proteins to arrive at homogeneous protein-protein bioconjugates.
FAU - Dhanjee, Heemal H
AU  - Dhanjee HH
AUID- ORCID: 0000-0002-3532-4430
AD  - Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, 
      Massachusetts 02139, United States.
FAU - Saebi, Azin
AU  - Saebi A
AD  - Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, 
      Massachusetts 02139, United States.
FAU - Buslov, Ivan
AU  - Buslov I
AD  - Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, 
      Massachusetts 02139, United States.
FAU - Loftis, Alexander R
AU  - Loftis AR
AD  - Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, 
      Massachusetts 02139, United States.
FAU - Buchwald, Stephen L
AU  - Buchwald SL
AUID- ORCID: 0000-0003-3875-4775
AD  - Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, 
      Massachusetts 02139, United States.
FAU - Pentelute, Bradley L
AU  - Pentelute BL
AUID- ORCID: 0000-0002-7242-801X
AD  - Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, 
      Massachusetts 02139, United States.
LA  - eng
GR  - F32 GM131592/GM/NIGMS NIH HHS/United States
GR  - P30 ES002109/ES/NIEHS NIH HHS/United States
GR  - R01 GM110535/GM/NIGMS NIH HHS/United States
GR  - U54 AI057159/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20200511
PL  - United States
TA  - J Am Chem Soc
JT  - Journal of the American Chemical Society
JID - 7503056
RN  - 0 (Organometallic Compounds)
RN  - 0 (Proteins)
RN  - 5TWQ1V240M (Palladium)
RN  - K848JZ4886 (Cysteine)
SB  - IM
EIN - J Am Chem Soc. 2020 Jul 15;142(28):12548. PMID: 32603593
MH  - Cysteine/*chemistry
MH  - Models, Molecular
MH  - Molecular Structure
MH  - Organometallic Compounds/*chemistry
MH  - Oxidation-Reduction
MH  - Palladium/*chemistry
MH  - Protein Binding
MH  - Proteins/*chemistry
PMC - PMC7586714
MID - NIHMS1633230
EDAT- 2020/05/05 06:00
MHDA- 2021/04/17 06:00
PMCR- 2021/05/20
CRDT- 2020/05/05 06:00
PHST- 2020/05/05 06:00 [pubmed]
PHST- 2021/04/17 06:00 [medline]
PHST- 2020/05/05 06:00 [entrez]
PHST- 2021/05/20 00:00 [pmc-release]
AID - 10.1021/jacs.0c03143 [doi]
PST - ppublish
SO  - J Am Chem Soc. 2020 May 20;142(20):9124-9129. doi: 10.1021/jacs.0c03143. Epub 
      2020 May 11.