PMID- 32364419
OWN - NLM
STAT- MEDLINE
DCOM- 20200806
LR  - 20220415
IS  - 1943-572X (Electronic)
IS  - 0003-4894 (Linking)
VI  - 129
IP  - 9
DP  - 2020 Sep
TI  - Prevalence and Prognostic Value of HPV among Tunisian Patients with Laryngeal 
      Cancer and Relationship between DNA HPV and p16, IGF-1R, Survivin, p53 
      Expressions.
PG  - 863-871
LID - 10.1177/0003489420918280 [doi]
AB  - OBJECTIVES: Tobacco and alcohol are the main etiological factors common to 
      laryngeal cancers. However, the Human Papilloma Virus (HPV) constitutes an 
      alternative risk factor according to several studies. In Tunisia, despite the 
      annual increasing incidence of laryngeal squamous cell carcinoma (LSCC), the 
      prevalence and prognostic significance of HPV have never been explored.In this 
      study, we sought to highlight HPV DNA in 70 biopsies of laryngeal cancer, and to 
      analyze the status of HPV infection in association with p53, p16, survivin, and 
      IGF-1R expressions. METHODS: HPV high risk (HPV HR) DNA was detected in tumors by 
      in situ hybridization. However, the expression of p53, p16, survivin and IGF-1R 
      were stained by immunohistochemistry test. The correlations of HPV status with 
      clinicopathological parameters, overall survival, disease-free survival and 
      proteins expressions were statistically evaluated. RESULTS: HPV HR DNA was 
      detected in 39 out of 70 (55.71%) laryngeal tumors. HPV+ patients have a better 
      overall survival (P = .081) and long disease-free-survival (P = .016) with a low 
      rate of recurrence (P = .006) than HPV(-) patients. No significant correlations 
      were found between HPV HR status and clinicopathological parameters (all P > 
      .005). Moreover, HPV+ tumors were not associated with expression of p53, p16 and 
      survivin. However, HPV HR status correlates with weak to moderate IGF-1R 
      expression (P = .043). CONCLUSION: The substantial detection of HPV HR in LSCC 
      tumors suggest that this virus plays an important part in laryngeal cancer in 
      Tunisia. It is a good prognostic factor. In addition, HPV infection could act to 
      block the pathway of IGF-1R expression.
FAU - Ben Elhadj, Mariem
AU  - Ben Elhadj M
AUID- ORCID: 0000-0002-1963-7752
AD  - Departement of Immuno-Histo-Cytology, Salah Azaiez Cancer Institute, Tunis, 
      Tunisia.
AD  - Research unit 17/ES/13 Faculty of Medicine, Tunis, Tunisia, University of Tunis 
      El Manar, Tunisia.
FAU - Fourati, Asma
AU  - Fourati A
AD  - Departement of Immuno-Histo-Cytology, Salah Azaiez Cancer Institute, Tunis, 
      Tunisia.
AD  - Research unit 17/ES/13 Faculty of Medicine, Tunis, Tunisia, University of Tunis 
      El Manar, Tunisia.
FAU - El Amine, Olfa
AU  - El Amine O
AD  - Departement of Immuno-Histo-Cytology, Salah Azaiez Cancer Institute, Tunis, 
      Tunisia.
FAU - Goucha, Aida
AU  - Goucha A
AD  - Departement of Immuno-Histo-Cytology, Salah Azaiez Cancer Institute, Tunis, 
      Tunisia.
FAU - El May, Ahmed
AU  - El May A
AD  - Departement of Immuno-Histo-Cytology, Salah Azaiez Cancer Institute, Tunis, 
      Tunisia.
FAU - El May, Michele-Veronique
AU  - El May MV
AD  - Research unit 17/ES/13 Faculty of Medicine, Tunis, Tunisia, University of Tunis 
      El Manar, Tunisia.
FAU - Mokni Baizig, Nehla
AU  - Mokni Baizig N
AUID- ORCID: 0000-0003-4703-6775
AD  - Departement of Immuno-Histo-Cytology, Salah Azaiez Cancer Institute, Tunis, 
      Tunisia.
AD  - Research unit 17/ES/13 Faculty of Medicine, Tunis, Tunisia, University of Tunis 
      El Manar, Tunisia.
LA  - eng
PT  - Journal Article
DEP - 20200504
PL  - United States
TA  - Ann Otol Rhinol Laryngol
JT  - The Annals of otology, rhinology, and laryngology
JID - 0407300
RN  - 0 (CDKN2A protein, human)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p16)
RN  - 0 (DNA, Viral)
RN  - 0 (IGF1R protein, human)
RN  - 0 (Survivin)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - EC 2.7.10.1 (Receptor, IGF Type 1)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Cyclin-Dependent Kinase Inhibitor p16/analysis/biosynthesis
MH  - DNA, Viral/analysis
MH  - Female
MH  - Humans
MH  - Laryngeal Neoplasms/chemistry/metabolism/mortality/*virology
MH  - Male
MH  - Middle Aged
MH  - Papillomaviridae/genetics
MH  - Papillomavirus Infections/complications/epidemiology/metabolism/*virology
MH  - Prevalence
MH  - Prognosis
MH  - Receptor, IGF Type 1/analysis/biosynthesis
MH  - Retrospective Studies
MH  - Survival Rate
MH  - Survivin/analysis/biosynthesis
MH  - Tumor Suppressor Protein p53/analysis/biosynthesis
MH  - Tunisia
OTO - NOTNLM
OT  - HPV-HR
OT  - IGF-1R
OT  - Tunisian patients
OT  - laryngeal tumors
OT  - prognostic factor
OT  - survivin
EDAT- 2020/05/05 06:00
MHDA- 2020/08/07 06:00
CRDT- 2020/05/05 06:00
PHST- 2020/05/05 06:00 [pubmed]
PHST- 2020/08/07 06:00 [medline]
PHST- 2020/05/05 06:00 [entrez]
AID - 10.1177/0003489420918280 [doi]
PST - ppublish
SO  - Ann Otol Rhinol Laryngol. 2020 Sep;129(9):863-871. doi: 10.1177/0003489420918280. 
      Epub 2020 May 4.