PMID- 32364675
OWN - NLM
STAT- MEDLINE
DCOM- 20210816
LR  - 20210816
IS  - 2053-1095 (Electronic)
IS  - 2053-1095 (Linking)
VI  - 6
IP  - 4
DP  - 2020 Nov
TI  - In ovo inoculation of duck embryos with different strains of Bacillus cereus to 
      analyse their synergistic post-hatch anti-allergic potentialities.
PG  - 992-999
LID - 10.1002/vms3.279 [doi]
AB  - BACKGROUND: Bacillus cereus is a Gram-positive, facultative anaerobic bacteria 
      with few strains reported to be used as probiotics for animals and birds in 
      recent times if the doses are formulated properly. OBJECTIVES: To analyse the 
      synergistic anti-allergic potentiality of different Bacillus cereus strains on 
      experimental in ovo and in vitro duck model, as probiotic immune stimulant. 
      MATERIALS AND METHODS: Different strains of Bacillus cereus from 29 isolates were 
      identified through 16S rRNA gene sequencing from the milk samples of buffalo 
      breeds of South Asia. The probiotic properties were tested in aspects of gram 
      staining, catalase test, coagulase, test, bile salt tolerance, pH tolerance and 
      phenol tolerance test. MIC(50) and MIC(90) levels were profiled using nine 
      different antibiotics, and antimicrobial activity against eight different enteric 
      pathogens was assessed. Finally, the test strains of Bacillus cereus (Colony 
      Forming Unit [CFU] 30X10(11) ) were combined-infused at different concentrations 
      in embryonated duck eggs to assess the post-hatch anti-allergic effects against 
      histamine-induced allergic reaction and their immunoglobulin E (IgE) level was 
      tested. RESULTS: Molecular identification confirmed the test strains as B. cereus 
      HKS 1-1, B. cereus LOCK 1,002 and B. cereus BF2, which were all motile, 
      spore-forming, catalase-positive and rod-shaped. All were 0.3% bile salt, 0.4% 
      phenol and pH tolerant. Two-way ANOVA test P values revealed that B. cereus BF2 
      was statistically significant (p < .0014) in bile salt tolerance test. B. cereus 
      HKS 1-1 was significant in phenol and pH tolerance at p < .0002 and p < .0489, 
      respectively. Besides, the test strains showed antibiotic sensitivity and 
      antimicrobial activity to different enteric pathogens. In vivo model referred the 
      test strains as effective in partial allergy reduction at same CFU but at 
      different concentrations with p < .0001 among the groups. CONCLUSION: The 
      isolated and characterized strains of B. cereus showed partial immune-stimulating 
      potentiality against experimentally induced allergic reaction.
CI  - (c) 2020 The Authors. Veterinary Medicine and Science Published by John Wiley & 
      Sons Ltd.
FAU - Al Azad, Salauddin
AU  - Al Azad S
AUID- ORCID: 0000-0002-3035-7882
AD  - Biotechnology and Genetic Engineering Discipline, Life Science School, Khulna 
      University, Khulna, Bangladesh.
FAU - Moazzem Hossain, Khondoker
AU  - Moazzem Hossain K
AD  - Biotechnology and Genetic Engineering Discipline, Life Science School, Khulna 
      University, Khulna, Bangladesh.
FAU - Rahman, Sana Mohammad Mahbubur
AU  - Rahman SMM
AD  - Biotechnology and Genetic Engineering Discipline, Life Science School, Khulna 
      University, Khulna, Bangladesh.
FAU - Al Mazid, Mohammad Faysal
AU  - Al Mazid MF
AD  - Biotechnology and Genetic Engineering Discipline, Life Science School, Khulna 
      University, Khulna, Bangladesh.
FAU - Barai, Pallob
AU  - Barai P
AD  - Biotechnology and Genetic Engineering Discipline, Life Science School, Khulna 
      University, Khulna, Bangladesh.
FAU - Gazi, Mohammad Shamim
AU  - Gazi MS
AD  - Biotechnology and Genetic Engineering Discipline, Life Science School, Khulna 
      University, Khulna, Bangladesh.
LA  - eng
PT  - Journal Article
DEP - 20200504
PL  - England
TA  - Vet Med Sci
JT  - Veterinary medicine and science
JID - 101678837
RN  - 0 (Anti-Allergic Agents)
SB  - IM
MH  - Animals
MH  - Anti-Allergic Agents/*administration & dosage
MH  - Bacillus cereus/*chemistry
MH  - Ducks/*microbiology
MH  - Embryo, Nonmammalian
MH  - Injections/veterinary
MH  - Ovum/drug effects/microbiology
MH  - Probiotics/*administration & dosage
PMC - PMC7738716
OTO - NOTNLM
OT  - Bacillus cereus
OT  - 16S rRNA gene sequencing
OT  - anti-allergic potentialities
OT  - antimicrobial activity
OT  - in ovo inoculation
OT  - probiotic properties
COIS- The authors have no competing interest.
EDAT- 2020/05/05 06:00
MHDA- 2021/08/17 06:00
PMCR- 2020/05/04
CRDT- 2020/05/05 06:00
PHST- 2020/02/03 00:00 [received]
PHST- 2020/04/09 00:00 [accepted]
PHST- 2020/05/05 06:00 [pubmed]
PHST- 2021/08/17 06:00 [medline]
PHST- 2020/05/05 06:00 [entrez]
PHST- 2020/05/04 00:00 [pmc-release]
AID - VMS3279 [pii]
AID - 10.1002/vms3.279 [doi]
PST - ppublish
SO  - Vet Med Sci. 2020 Nov;6(4):992-999. doi: 10.1002/vms3.279. Epub 2020 May 4.