PMID- 32365745
OWN - NLM
STAT- MEDLINE
DCOM- 20210209
LR  - 20240328
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 25
IP  - 9
DP  - 2020 Apr 30
TI  - Polydopamine Nanosphere with In-Situ Loaded Gentamicin and Its Antimicrobial 
      Activity.
LID - 10.3390/molecules25092090 [doi]
LID - 2090
AB  - The mussel inspired polydopamine has acquired great relevance in the field of 
      nanomedicines, owing to its incredible physicochemical properties. Polydopamine 
      nanoparticles (PDA NPs) due to their low cytotoxicity, high biocompatibility and 
      ready biodegradation have already been widely investigated in various drug 
      delivery, chemotherapeutic, and diagnostic applications. In addition, owing to 
      its highly reactive nature, it possesses a very high capability for loading drugs 
      and chemotherapeutics. Therefore, the loading efficiency of PDA NPs for an 
      antibiotic i.e., gentamicin (G) has been investigated in this work. For this 
      purpose, an in-situ polymerization method was studied to load the drug into PDA 
      NPs using variable drug: monomer ratios. Scanning electron microscope (SEM), 
      Fourier-transform infrared spectroscopy (FTIR), and X-ray photoelectron 
      spectroscopy (XPS) confirmed the successful loading of drug within PDA NPs, 
      mainly via hydrogen bonding between the amine groups of gentamicin and the 
      hydroxyl groups of PDA. The loading amount was quantified by liquid 
      chromatography-mass spectrometry (LC-MS) and the highest percentage loading 
      capacity was achieved for G-PDA prepared with drug to monomer ratio of 1:1. 
      Moreover, the gentamicin loaded PDA NPs were tested in a preliminary 
      antibacterial evaluation using the broth microdilution method against both 
      Gram-(+) Staphylococcus aureus and Gram-(-) Pseudomonas aeruginosa 
      microorganisms. The highest loaded G-PDA sample exhibited the lowest minimum 
      inhibitory concentration and minimum bactericidal concentration values. The 
      developed gentamicin loaded PDA is very promising for long term drug release and 
      treating various microbial infections.
FAU - Batul, Rahila
AU  - Batul R
AUID- ORCID: 0000-0002-5933-0704
AD  - Department of Chemistry and Biotechnology, Faculty of Science, Engineering and 
      Technology, Swinburne University of Technology, Hawthorn, VIC 3122, Australia.
FAU - Bhave, Mrinal
AU  - Bhave M
AD  - Department of Chemistry and Biotechnology, Faculty of Science, Engineering and 
      Technology, Swinburne University of Technology, Hawthorn, VIC 3122, Australia.
FAU - J Mahon, Peter
AU  - J Mahon P
AD  - Department of Chemistry and Biotechnology, Faculty of Science, Engineering and 
      Technology, Swinburne University of Technology, Hawthorn, VIC 3122, Australia.
FAU - Yu, Aimin
AU  - Yu A
AD  - Department of Chemistry and Biotechnology, Faculty of Science, Engineering and 
      Technology, Swinburne University of Technology, Hawthorn, VIC 3122, Australia.
LA  - eng
PT  - Journal Article
DEP - 20200430
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Drug Carriers)
RN  - 0 (Gentamicins)
RN  - 0 (Indoles)
RN  - 0 (Polymers)
RN  - 0 (polydopamine)
SB  - IM
MH  - Anti-Bacterial Agents/*administration & dosage
MH  - Bacteria/drug effects
MH  - Chemistry Techniques, Synthetic
MH  - Drug Carriers/*chemistry
MH  - Drug Delivery Systems
MH  - Drug Liberation
MH  - Gentamicins/*administration & dosage
MH  - Indoles/*chemistry
MH  - Microbial Sensitivity Tests
MH  - Nanospheres/*chemistry
MH  - Polymers/*chemistry
MH  - Spectrum Analysis
PMC - PMC7250025
OTO - NOTNLM
OT  - antimicrobial property
OT  - drug delivery
OT  - gentamicin
OT  - polydopamine
COIS- The authors declare no conflict of interest.
EDAT- 2020/05/06 06:00
MHDA- 2021/02/10 06:00
PMCR- 2020/04/30
CRDT- 2020/05/06 06:00
PHST- 2020/03/20 00:00 [received]
PHST- 2020/04/25 00:00 [revised]
PHST- 2020/04/25 00:00 [accepted]
PHST- 2020/05/06 06:00 [entrez]
PHST- 2020/05/06 06:00 [pubmed]
PHST- 2021/02/10 06:00 [medline]
PHST- 2020/04/30 00:00 [pmc-release]
AID - molecules25092090 [pii]
AID - molecules-25-02090 [pii]
AID - 10.3390/molecules25092090 [doi]
PST - epublish
SO  - Molecules. 2020 Apr 30;25(9):2090. doi: 10.3390/molecules25092090.