PMID- 32370225 OWN - NLM STAT- MEDLINE DCOM- 20210208 LR - 20210208 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 21 IP - 9 DP - 2020 May 2 TI - The Interaction of lncRNA XLOC-2222497, AKR1C1, and Progesterone in Porcine Endometrium and Pregnancy. LID - 10.3390/ijms21093232 [doi] LID - 3232 AB - The endometrium is an important tissue for pregnancy and plays an important role in reproduction. In this study, high-throughput transcriptome sequencing was performed in endometrium samples of Meishan and Yorkshire pigs on days 18 and 32 of pregnancy. Aldo-keto reductase family 1 member C1 (AKR1C1) was found to be a differentially expressed gene, and was identified by quantitative real-time PCR (qRT-PCR) and Western blot. Immunohistochemistry results revealed the cellular localization of the AKR1C1 protein in the endometrium. Luciferase activity assay demonstrated that the AKR1C1 core promoter region was located in the region from -706 to -564, containing two nuclear factor erythroid 2-related factor 2 (NRF2) binding sites (antioxidant response elements, AREs). XLOC-2222497 was identified as a nuclear long non-coding RNA (lncRNA) highly expressed in the endometrium. XLOC-2222497 overexpression and knockdown have an effect on the expression of AKR1C1. Endocrinologic measurement showed the difference in progesterone levels between Meishan and Yorkshire pigs. Progesterone treatment upregulated AKR1C1 and XLOC-2222497 expression in porcine endometrial epithelial cells. In conclusion, transcriptome analysis revealed differentially expressed transcripts during the early pregnancy process. Further experiments demonstrated the interaction of XLOC-2222497/AKR1C1/progesterone in the endometrium and provided new potential targets for pregnancy maintenance and its control. FAU - Su, Tao AU - Su T AD - Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, China. AD - Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China. AD - Colleges of Animal Science & Technology, Huazhong Agricultural University, Wuhan 430070, China. FAU - Yu, Haile AU - Yu H AD - Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, China. AD - Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China. AD - Colleges of Animal Science & Technology, Huazhong Agricultural University, Wuhan 430070, China. FAU - Luo, Gan AU - Luo G AD - Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, China. AD - Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China. AD - Colleges of Animal Science & Technology, Huazhong Agricultural University, Wuhan 430070, China. FAU - Wang, Mengxia AU - Wang M AD - Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, China. AD - Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China. AD - Colleges of Animal Science & Technology, Huazhong Agricultural University, Wuhan 430070, China. FAU - Zhou, Changfan AU - Zhou C AD - Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, China. AD - Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China. AD - Colleges of Animal Science & Technology, Huazhong Agricultural University, Wuhan 430070, China. FAU - Zhang, Long AU - Zhang L AD - Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, China. AD - Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China. AD - Colleges of Animal Science & Technology, Huazhong Agricultural University, Wuhan 430070, China. FAU - Hou, Bin AU - Hou B AD - Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, China. AD - Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China. AD - Colleges of Animal Science & Technology, Huazhong Agricultural University, Wuhan 430070, China. FAU - Zhang, Chi AU - Zhang C AD - Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, China. AD - College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. FAU - Liu, Min AU - Liu M AD - Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, China. AD - College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. FAU - Xu, Dequan AU - Xu D AD - Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, China. AD - Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China. AD - Colleges of Animal Science & Technology, Huazhong Agricultural University, Wuhan 430070, China. LA - eng GR - 2014CB138504/State Key Development Program for Basic Research of China/ GR - 2016ZX08006002/National Major Science and Technology Projects of China/ GR - 31201764/National Natural Science Foundation of China/ GR - 31172179/National Natural Science Foundation of China/ GR - 2015CFA101/Natural Science Foundation of Hubei Province/ GR - 2018skjcx01/Agricultural Science Innovation Foundation of Hubei Province/ GR - DX/Animal Husbandry Major Technology Collaborative Extension Project of Hubei Province/ GR - DX/Agricultural Science and Technology Innovation Action of Hubei Province/ GR - DX/Research Project of Huazhong Agricultural University, China/ PT - Journal Article DEP - 20200502 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (RNA, Long Noncoding) RN - 0 (RNA, Messenger) RN - 4G7DS2Q64Y (Progesterone) RN - EC 1.1.1.- (20-Hydroxysteroid Dehydrogenases) RN - EC 1.1.1.- (3 alpha-beta, 20 beta-hydroxysteroid dehydrogenase) SB - IM MH - 20-Hydroxysteroid Dehydrogenases/*genetics/metabolism MH - Animals MH - Blotting, Western MH - Cells, Cultured MH - Endometrium/cytology/*metabolism MH - Epithelial Cells/metabolism MH - Female MH - Gene Expression Profiling/*methods MH - *Gene Expression Regulation MH - Pregnancy MH - Progesterone/*metabolism MH - RNA, Long Noncoding/*genetics MH - RNA, Messenger/genetics/metabolism MH - Reverse Transcriptase Polymerase Chain Reaction MH - Swine PMC - PMC7247569 OTO - NOTNLM OT - AKR1C1 OT - endometrium OT - lncRNA OT - pig OT - progesterone COIS- The authors declare no conflict of interest. EDAT- 2020/05/07 06:00 MHDA- 2021/02/09 06:00 PMCR- 2020/05/01 CRDT- 2020/05/07 06:00 PHST- 2020/03/05 00:00 [received] PHST- 2020/04/26 00:00 [revised] PHST- 2020/04/28 00:00 [accepted] PHST- 2020/05/07 06:00 [entrez] PHST- 2020/05/07 06:00 [pubmed] PHST- 2021/02/09 06:00 [medline] PHST- 2020/05/01 00:00 [pmc-release] AID - ijms21093232 [pii] AID - ijms-21-03232 [pii] AID - 10.3390/ijms21093232 [doi] PST - epublish SO - Int J Mol Sci. 2020 May 2;21(9):3232. doi: 10.3390/ijms21093232.