PMID- 32370602
OWN - NLM
STAT- MEDLINE
DCOM- 20210728
LR  - 20210728
IS  - 1748-6963 (Electronic)
IS  - 1743-5889 (Linking)
VI  - 15
IP  - 12
DP  - 2020 May
TI  - Novel intracellular antibiotic delivery system against Staphylococcus aureus: 
      cloxacillin-loaded poly(d,l-lactide-co-glycolide) acid nanoparticles.
PG  - 1189-1203
LID - 10.2217/nnm-2019-0371 [doi]
AB  - Aim: First, to compare in vitro minimum inhibitory concentrations (MIC) of free 
      cloxacillin and cloxacillin-containing nanoparticles (NP) against 
      methicillin-susceptible (MSSA) and resistant Staphylococcus aureus (MRSA) and 
      second, to assess NP antimicrobial activity against intracellular S. aureus. 
      Methods: Poly(d,l-lactide-co-glycolide) acid (PLGA)-NP were loaded with 
      cloxacillin and physico-chemically characterized. MICs were determined for 
      reference strains Newman-(MSSA) and USA300-(MRSA). Murine alveolar macrophages 
      were infected, and bacterial intracellular survival was assessed after incubating 
      with free-cloxacillin or PLGA-cloxacillin-NP. Results & conclusion: For both 
      isolates, MICs for antibiotic-loaded-NP were lower than those obtained with free 
      cloxacillin, indicating that the drug encapsulation improves antimicrobial 
      activity. A sustained antibiotic release was demonstrated when using the 
      PLGA-cloxacillin-NP. When considering the lowest concentrations, the use of 
      drug-loaded NP enabled a higher reduction of intracellular bacterial load.
FAU - Lacoma, Alicia
AU  - Lacoma A
AUID- ORCID: 0000-0002-2049-3872
AD  - Microbiology Department, Hospital Universitari Germans Trias i Pujol, Fundacio 
      Institut d'Investigacio en Ciencies de la Salut GermansTrias i Pujol, Universitat 
      Autonoma de Barcelona, Badalona, Spain.
AD  - CIBER Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, Spain.
FAU - Uson, Laura
AU  - Uson L
AD  - Institute of Nanoscience of Aragon (INA), Department of Chemical Engineering & 
      Environmental Technologies, University of Zaragoza & Instituto de Ciencia de 
      Materiales de Aragon (ICMA), Universidad de Zaragoza-CSIC, 50018, Zaragoza, 
      Spain.
AD  - CIBER Bioingenieria, Biomateriales y Nanomedicina (CIBER-BBN), Instituto de Salud 
      Carlos III, Spain.
FAU - Mendoza, Gracia
AU  - Mendoza G
AD  - Institute of Nanoscience of Aragon (INA), Department of Chemical Engineering & 
      Environmental Technologies, University of Zaragoza & Instituto de Ciencia de 
      Materiales de Aragon (ICMA), Universidad de Zaragoza-CSIC, 50018, Zaragoza, 
      Spain.
AD  - CIBER Bioingenieria, Biomateriales y Nanomedicina (CIBER-BBN), Instituto de Salud 
      Carlos III, Spain.
FAU - Sebastian, Victor
AU  - Sebastian V
AD  - Institute of Nanoscience of Aragon (INA), Department of Chemical Engineering & 
      Environmental Technologies, University of Zaragoza & Instituto de Ciencia de 
      Materiales de Aragon (ICMA), Universidad de Zaragoza-CSIC, 50018, Zaragoza, 
      Spain.
AD  - CIBER Bioingenieria, Biomateriales y Nanomedicina (CIBER-BBN), Instituto de Salud 
      Carlos III, Spain.
FAU - Garcia-Garcia, Esther
AU  - Garcia-Garcia E
AD  - Microbiology Department, Hospital Universitari Germans Trias i Pujol, Fundacio 
      Institut d'Investigacio en Ciencies de la Salut GermansTrias i Pujol, Universitat 
      Autonoma de Barcelona, Badalona, Spain.
FAU - Muriel-Moreno, Beatriz
AU  - Muriel-Moreno B
AD  - Microbiology Department, Hospital Universitari Germans Trias i Pujol, Fundacio 
      Institut d'Investigacio en Ciencies de la Salut GermansTrias i Pujol, Universitat 
      Autonoma de Barcelona, Badalona, Spain.
FAU - Dominguez, Jose
AU  - Dominguez J
AD  - Microbiology Department, Hospital Universitari Germans Trias i Pujol, Fundacio 
      Institut d'Investigacio en Ciencies de la Salut GermansTrias i Pujol, Universitat 
      Autonoma de Barcelona, Badalona, Spain.
AD  - CIBER Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, Spain.
FAU - Arruebo, Manuel
AU  - Arruebo M
AD  - Institute of Nanoscience of Aragon (INA), Department of Chemical Engineering & 
      Environmental Technologies, University of Zaragoza & Instituto de Ciencia de 
      Materiales de Aragon (ICMA), Universidad de Zaragoza-CSIC, 50018, Zaragoza, 
      Spain.
AD  - CIBER Bioingenieria, Biomateriales y Nanomedicina (CIBER-BBN), Instituto de Salud 
      Carlos III, Spain.
FAU - Prat, Cristina
AU  - Prat C
AD  - Microbiology Department, Hospital Universitari Germans Trias i Pujol, Fundacio 
      Institut d'Investigacio en Ciencies de la Salut GermansTrias i Pujol, Universitat 
      Autonoma de Barcelona, Badalona, Spain.
AD  - CIBER Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, Spain.
AD  - Julius Centre for Health Sciences and Primary Care, University Medical Center 
      Utrecht, Utrecht University, Utrecht, The Netherlands.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200506
PL  - England
TA  - Nanomedicine (Lond)
JT  - Nanomedicine (London, England)
JID - 101278111
RN  - 0 (Anti-Bacterial Agents)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - O6X5QGC2VB (Cloxacillin)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/pharmacology/therapeutic use
MH  - Cloxacillin
MH  - *Methicillin-Resistant Staphylococcus aureus
MH  - Mice
MH  - Microbial Sensitivity Tests
MH  - *Nanoparticles
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Staphylococcus aureus
OTO - NOTNLM
OT  - Staphylococcus aureus
OT  - antimicrobial
OT  - drug delivery
OT  - intracellular infection
OT  - nanoparticle
EDAT- 2020/05/07 06:00
MHDA- 2021/07/29 06:00
CRDT- 2020/05/07 06:00
PHST- 2020/05/07 06:00 [pubmed]
PHST- 2021/07/29 06:00 [medline]
PHST- 2020/05/07 06:00 [entrez]
AID - 10.2217/nnm-2019-0371 [doi]
PST - ppublish
SO  - Nanomedicine (Lond). 2020 May;15(12):1189-1203. doi: 10.2217/nnm-2019-0371. Epub 
      2020 May 6.