PMID- 32372213
OWN - NLM
STAT- MEDLINE
DCOM- 20210712
LR  - 20210712
IS  - 1432-0738 (Electronic)
IS  - 0340-5761 (Print)
IS  - 0340-5761 (Linking)
VI  - 94
IP  - 6
DP  - 2020 Jun
TI  - Electronic cigarette vapour moderately stimulates pro-inflammatory signalling 
      pathways and interleukin-6 production by human monocyte-derived dendritic cells.
PG  - 2097-2112
LID - 10.1007/s00204-020-02757-8 [doi]
AB  - Dendritic cells (DCs) are professional antigen presenting cells that play a 
      critical role in bridging innate and adaptive immunity. Numerous studies have 
      shown that tobacco constituents present in conventional cigarettes affect the 
      phenotype and function of DCs; however, no studies have examined the effects of 
      vapour from E-cigarettes on human DCs. Here, the effects of E-cigarette vapour 
      extract (ECVE) on the phenotype and function of DCs were investigated by creating 
      an in vitro cell culture model using human monocyte-derived DCs (MoDCs). Immature 
      DCs were generated from peripheral blood monocytes and mature DCs were then 
      produced by treatment with LPS or Poly I:C for 24 h. For LPS-matured DCs, 3% ECVE 
      treatment slightly suppressed HLA-DR and CD86 expression, whereas 1% ECVE 
      treatment enhanced IL-6 production. The overall expression of 29 signalling 
      molecules and other cytoplasmic proteins (mainly associated with DC activation) 
      was significantly upregulated in immature DCs by 1% ECVE, and in LPS-treated DCs 
      by 3% ECVE. In particular, the condition that induced IL-6 production also 
      upregulated MAPK pathway activation. These findings indicate that E-cigarette 
      vapour moderately affects human DCs, but the effects are less pronounced than 
      those reported for tobacco smoke.
FAU - Chen, I-Ling
AU  - Chen IL
AD  - School of Life Sciences, University of Nottingham, Life Sciences Building, 
      University Park, Nottingham, NG7 2RD, UK.
FAU - Todd, Ian
AU  - Todd I
AD  - School of Life Sciences, University of Nottingham, Life Sciences Building, 
      University Park, Nottingham, NG7 2RD, UK.
FAU - Tighe, Patrick J
AU  - Tighe PJ
AD  - School of Life Sciences, University of Nottingham, Life Sciences Building, 
      University Park, Nottingham, NG7 2RD, UK.
FAU - Fairclough, Lucy C
AU  - Fairclough LC
AUID- ORCID: 0000-0003-4581-9741
AD  - School of Life Sciences, University of Nottingham, Life Sciences Building, 
      University Park, Nottingham, NG7 2RD, UK. lucy.fairclough@nottingham.ac.uk.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200505
PL  - Germany
TA  - Arch Toxicol
JT  - Archives of toxicology
JID - 0417615
RN  - 0 (E-Cigarette Vapor)
RN  - 0 (IL6 protein, human)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Interleukin-6)
SB  - IM
MH  - Cells, Cultured
MH  - Dendritic Cells/*drug effects/immunology/metabolism
MH  - E-Cigarette Vapor/*toxicity
MH  - *Electronic Nicotine Delivery Systems
MH  - Humans
MH  - Inflammation Mediators/*metabolism
MH  - Interleukin-6/*metabolism
MH  - Phenotype
MH  - Signal Transduction
MH  - Up-Regulation
MH  - Vaping/*adverse effects
PMC - PMC7303083
OTO - NOTNLM
OT  - Dendritic cell
OT  - Electronic cigarette
OT  - Interleukin-6
OT  - Protein array
OT  - Signalling molecule
COIS- The authors state that they have no conflicts of interest to declare.
EDAT- 2020/05/07 06:00
MHDA- 2021/07/13 06:00
PMCR- 2020/05/05
CRDT- 2020/05/07 06:00
PHST- 2020/04/09 00:00 [received]
PHST- 2020/04/21 00:00 [accepted]
PHST- 2020/05/07 06:00 [pubmed]
PHST- 2021/07/13 06:00 [medline]
PHST- 2020/05/07 06:00 [entrez]
PHST- 2020/05/05 00:00 [pmc-release]
AID - 10.1007/s00204-020-02757-8 [pii]
AID - 2757 [pii]
AID - 10.1007/s00204-020-02757-8 [doi]
PST - ppublish
SO  - Arch Toxicol. 2020 Jun;94(6):2097-2112. doi: 10.1007/s00204-020-02757-8. Epub 
      2020 May 5.